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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS02595 [old locus tag: SACOL0514 ]
  • pan locus tag?: SAUPAN002186000
  • symbol: SACOL_RS02595
  • pan gene symbol?: gltB
  • synonym:
  • product: glutamate synthase large subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS02595 [old locus tag: SACOL0514 ]
  • symbol: SACOL_RS02595
  • product: glutamate synthase large subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 513658..518157
  • length: 4500
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (513658..518157) NCBI
  • BioCyc: SACOL_RS02595 BioCyc
  • MicrobesOnline: see SACOL0514

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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    ATGCACAATGAGAAATTAATTAAAGGCTTATATGACTATCGTGAGGAACATGATGCGTGT
    GGTATTGGTTTTTATGCGAATATGGATAATAAAAGGTCTCACGACATCATTGATAAATCG
    CTTGAAATGTTGCGACGCTTAGATCACAGGGGCGGGGTCGGCGCAGATGGCATCACTGGT
    GATGGCGCAGGTATTATGACTGAAATACCTTTTGCATTTTTCAAACAACATGTAACGGAC
    TTTGATATCCCAGGTGAAGGTGAATATGCCGTGGGGTTATTTTTTTCCAAAGAACGCATT
    TTAGGTTCTGAACATGAAGTAGTTTTTAAAAAATATTTTGAAGGCGAAGGGTTATCAATT
    CTTGGTTATCGTAATGTACCAGTTAATAAAGATGCCATTGCTAAACATGTAGCAGATACG
    ATGCCAGTCATTCAACAAGTGTTTATTGATATTAGGGACATTGAAGATGTTGAAAAGCGT
    TTGTTTTTAGCGAGAAAACAATTAGAGTTCTATTCGACTCAGTGCGATTTAGAATTGTAT
    TTTACGAGCTTATCACGCAAAACAATTGTATATAAAGGTTGGTTACGATCAGACCAAATT
    AAAAAAGTATATACAGATTTATCGGATGATTTATATCAATCAAAGCTAGGGTTAGTGCAT
    TCGAGATTTAGTACGAATACATTCCCGAGTTGGAAAAGGGCACATCCTAACCGTATGTTA
    ATGCATAATGGTGAGATTAACACGATTAAAGGTAATGTAAACTGGATGCGAGCACGCCAA
    CATAAATTAATCGAAACATTATTTGGCGAGGATCAACATAAAGTGTTTCAAATTGTCGAT
    GAGGATGGTAGTGACTCTGCCATTGTAGATAATGCGCTAGAGTTCTTATCGTTAGCCATG
    GAGCCAGAAAAGGCAGCGATGTTACTCATACCTGAACCTTGGTTATATAATGAAGCGAAT
    GATGCAAATGTACGTGCGTTTTATGAATTTTATAGTTATTTAATGGAACCGTGGGATGGT
    CCTACAATGATTTCGTTCTGTAACGGTGACAAACTTGGCGCGCTTACAGATAGAAATGGA
    TTACGTCCAGGTCGTTATACGATTACTAAAGATAACTTTATTGTCTTTTCATCTGAAGTG
    GGTGTTGTGGACGTACCTGAAAGTAATGTTGCTTTTAAAGGTCAATTGAATCCTGGAAAG
    TTATTGCTTGTTGATTTTAAACAGAATAAAGTCATTGAAAATAATGATTTAAAAGGTGCG
    ATTGCTGGAGAATTACCATATAAAGCGTGGATTGATAACCATAAAGTTGACTTTGATTTT
    GAAAATATACAATATCAAGATTCGCAATGGAAAGATGAGACGTTATTTAAATTACAACGT
    CAGTTTGCATACACGAAAGAAGAGATTCATAAGTATATTCAGGAACTTGTAGAAGGTAAG
    AAGGATCCTATCGGTGCAATGGGATATGATGCGCCAATTGCAGTGTTGAACGAGCGACCA
    GAATCACTATTTAATTACTTTAAACAGCTGTTTGCACAAGTTACGAATCCACCAATTGAT
    GCGTATCGTGAAAAAATCGTAACGAGTGAACTTTCTTATTTAGGTGGCGAAGGTAACTTA
    CTAGCACCTGACGAAACGGTTTTAGATCGTATTCAATTGAAAAGGCCGGTATTGAATGAA
    TCACACTTAGCAGCGATTGATCAGGAACATTTTAAATTAACTTATTTATCAACGGTATAT
    GAAGGGGATTTGGAAGATGCGTTAGAAGCATTAGGCCGAGAAGCAGTGAATGCTGTAAAG
    CAAGGCGCTCAAATTCTAGTGTTAGATGATAGTGGATTAGTTGATAGCAATGGCTTTGCA
    ATGCCGATGTTACTCGCAATAAGTCATGTGCATCAATTACTTATTAAAGCAGATTTACGT
    ATGTCTACAAGTTTAGTCGCTAAATCTGGTGAGACACGAGAAGTGCATCATGTTGCTTGT
    TTACTCGCATATGGCGCGAATGCAATTGTGCCATACCTAGCGCAACGTACAGTTGAACAA
    CTGACATTGACAGAAGGGTTACAAGGCACCGTTGTCGATAATGTTAAGACATATACGGAT
    GTATTGTCAGAAGGTGTCATTAAAGTAATGGCTAAGATGGGAATTTCGACAGTGCAAAGT
    TATCAAGGGGCACAAATATTTGAAGCGATTGGCTTGTCTCATGATGTGATTGATCGTTAT
    TTTACTGGGACACAGTCTAAGTTATCTGGTATTTCGATTGATCAAATTGATGCTGAAAAT
    AAAGCACGTCAACAAAGTGATGATAATTATCTTGCATCAGGTAGTACATTCCAATGGAGA
    CAACAAGGTCAACATCATGCTTTTAATCCGGAATCTATTTTCTTATTGCAGCACGCATGT
    AAAGAAAATGACTATGCGCAATTTAAAGCATACTCTGAAGCGGTGAACAAAAATAGAACA
    GATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTT
    GAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATT
    TCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAAT
    AGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAGTTGATGGAAGCAACAAA
    GTAAGTGCGATTAAACAAGTTGCTTCTGGGCGTTTTGGTGTAACTAGTGATTATTTACAA
    CATGCCAAAGAAATTCAAATTAAAGTTGCGCAAGGTGCAAAGCCTGGTGAAGGTGGTCAA
    TTACCTGGTACTAAGGTATATCCGTGGATTGCGAAGACAAGAGGGTCAACGCCAGGTATC
    GGTCTGATTTCACCACCGCCACATCATGATATTTATTCAATAGAAGATTTAGCGCAACTG
    ATACATGATTTGAAAAATGCGAATAAAGATGCAGATATCGCGGTAAAATTAGTTTCGAAA
    ACAGGTGTTGGTACCATTGCATCTGGGGTGGCAAAAGCATTTGCAGATAAAATTGTCATC
    AGTGGTTACGATGGTGGTACAGGGGCTTCACCCAAAACGAGTATTCAGCATGCCGGTGTT
    CCTTGGGAGATTGGTTTAGCAGAAACACATCAAACATTAAAACTAAATGACTTAAGAAGT
    CGTGTTAAGTTAGAAACAGACGGTAAGTTATTAACTGGTAAAGATGTAGCGTACGCATGT
    GCGCTTGGAGCGGAAGAATTTGGATTTGCAACTGCACCATTAGTGGTGTTGGGCTGTATT
    ATGATGCGTGTATGCCATAAAGATACATGTCCAGTAGGAGTTGCAACTCAAAACAAAGAT
    TTACGTGCTTTATATAGAGGTAAAGCACATCATGTTGTTAATTTTATGCATTTTATTGCA
    CAAGAATTAAGAGAAATTTTAGCATCTTTAGGTTTGAAACGTGTAGAAGACTTAGTTGGA
    AGAACTGATTTATTACAACGATCATCAACATTAAAAGCGAATAGCAAAGCGGCTAGTATT
    GATGTTGAAAAACTGTTATGTCCTTTCGATGGGCCAAACACAAAAGAAATTCAACAAAAT
    CATAATCTTGAGCATGGATTTGATTTAACAAATTTATATGAAGTAACGAAGCCATATATT
    GCTGAAGGGCGTCGCTATACAGGTAGCTTTACAGTAAATAATGAACAACGTGATGTAGGG
    GTTATTACAGGTAGTGAGATTTCGAAACAATATGGAGAAGCAGGACTTCCTGAAAATACA
    ATTAATGTTTATACGAATGGTCATGCTGGTCAAAGTCTTGCAGCATATGCACCGAAAGGC
    TTAATGATTCATCATACTGGAGATGCGAATGACTATGTTGGTAAAGGATTATCTGGTGGT
    ACGGTCATTGTCAAAGCACCTTTTGAAGAACGACAAAATGAAATTATTGCTGGTAACGTC
    TCATTCTATGGTGCGACAAGTGGTAAGGCATTTATTAACGGTAGTGCAGGAGAAAGATTC
    TGTATTAGAAATAGTGGTGTAGATGTTGTCGTTGAAGGTATCGGCGACCATGGATTAGAG
    TATATGACTGGTGGACATGTCATTAATTTAGGTGATGTAGGTAAGAACTTCGGTCAAGGT
    ATGAGTGGTGGTATTGCTTACGTTATCCCGTCTGATGTAGAAGCTTTTGTTGAAAATAAT
    CAACTAGATACGCTTTCGTTTACAAAGATTAAACACCAAGAAGAAAAAGCATTCATTAAG
    CAAATGCTGGAAGAACATGTGTCACACACGAATAGTACGAGAGCGATTCATGTGTTAAAA
    CATTTTGATCGCATTGAAGATGTCGTCGTTAAAGTTATTCCTAAAGATTATCAATTAATG
    ATGCAAAAAATTCATTTGCACAAATCATTACATGACAATGAAGATGAAGCGATGTTAGCT
    GCATTTTACGATGACAGTAAAACAATCGATGCTAAACATAAACCAGCCGTTGTGTATTAA
    60
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS02595 [old locus tag: SACOL0514 ]
  • symbol: SACOL_RS02595
  • description: glutamate synthase large subunit
  • length: 1499
  • theoretical pI: 5.97235
  • theoretical MW: 166333
  • GRAVY: -0.315944

⊟Function[edit | edit source]

  • reaction:
    EC 1.4.1.13?  ExPASy
    Glutamate synthase (NADPH) 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH
  • TIGRFAM:
    formylmethanofuran dehydrogenase subunit C (TIGR03122; EC 1.2.99.5; HMM-score: 25.9)
  • TheSEED: see SACOL0514
  • PFAM:
    TIM_barrel (CL0036) Glu_synthase; Conserved region in glutamate synthase (PF01645; HMM-score: 521.3)
    NTN (CL0052) GATase_2; Glutamine amidotransferases class-II (PF00310; HMM-score: 513.7)
    and 3 more
    TIM_barrel (CL0036) Glu_syn_central; Glutamate synthase central domain (PF04898; HMM-score: 330.4)
    no clan defined GXGXG; GXGXG motif (PF01493; HMM-score: 225.1)
    TIM_barrel (CL0036) FMN_dh; FMN-dependent dehydrogenase (PF01070; HMM-score: 9.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.78
    • Cytoplasmic Membrane Score: 8.16
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.029572
    • TAT(Tat/SPI): 0.000731
    • LIPO(Sec/SPII): 0.001237
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEMLRRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILGSEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKRLFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKVYTDLSDDLYQSKLGLVHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVFQIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYLMEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAFKGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQWKDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFKQLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAIDQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPMLLAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLTLTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRYFTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQHACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMSYGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGVTSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIYSIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGASPKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFGFATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREILASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLEHGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTINVYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNVSFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFGQGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAIHVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHKPAVVY

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 1.56 1.57 1.58 1.59 1.60 1.61 1.62 1.63 1.64 1.65 1.66 1.67 1.68 1.69 1.70 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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