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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1995 [new locus tag: SACOL_RS10425 ]
  • pan locus tag?: SAUPAN004998000
  • symbol: SACOL1995
  • pan gene symbol?: pmtB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1995 [new locus tag: SACOL_RS10425 ]
  • symbol: SACOL1995
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2056737..2057417
  • length: 681
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGGCATTGTTAATTAGGAATTTTAAATTACGACGATATACACTTATCATCTATGTT
    CTATTATTAACCTTATATCCTTTTTATATAATGCTTGACTCAACAAAGTTCTTTTACTTA
    TTGCAATCTTTTATAAGTCCAACAATATTGATTATTTGGATTTTAGATGCTGGTCACTTA
    TTTAGACTCAATCGTAGATTAGGTGGTAATGATTCATATTATTTTTATATGAGTTTACCA
    GTGTCAAAAAAACAATTGCTTAATGCCAACTATATTACATGCATTGTTTTAACATTAATT
    GGAACGCTTGTTATTAGTTTATATGCTTATGAAGCAGATGTGATTGAACCAAATTCAATC
    TATTTTTCAACTGCATATGCATTTGTCATATCTAACTTCTTGTCTATACCAATTGCATTT
    AGTCAATTTACAGAATTGCGTAGAGTCAAAGTGCCATATGGTATATACGTGTTTACTATT
    ATCATTTTAGTTCCATTTTTATTTTCAATTGCAATAGTATTGGTGAATTATTTTGTTCTA
    AGTCAATCATCATTCCCAGATTTATATTCATATATTTTAAATATTGGTTTTCTAATTATA
    AGCATTGTTATACTTATTGTTAATTATTTTAAACAGCTCAATAAAATAAATACTAGAAAA
    TTTAAAGGAGGCAGTCGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    681

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1995 [new locus tag: SACOL_RS10425 ]
  • symbol: SACOL1995
  • description: hypothetical protein
  • length: 226
  • theoretical pI: 10.0187
  • theoretical MW: 26293.2
  • GRAVY: 0.784956

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01108151: hypothetical protein
  • PFAM:
    ABC-2 (CL0181) ABC2_membrane_5; ABC-2 family transporter protein (PF13346; HMM-score: 94.5)
    and 2 more
    ABC2_membrane_2; ABC-2 family transporter protein (PF12679; HMM-score: 15)
    no clan defined DUF441; Protein of unknown function (DUF441) (PF04284; HMM-score: 7.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002953
    • TAT(Tat/SPI): 0.000125
    • LIPO(Sec/SPII): 0.001661
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSFISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGTLVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTIIILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINTRKFKGGSR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PmtR* (repression) regulon
    PmtR*(TF)important in Hypothetical ABC transporter; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Som S Chatterjee, Hwang-Soo Joo, Anthony C Duong, Thomas D Dieringer, Vee Y Tan, Yan Song, Elizabeth R Fischer, Gordon Y C Cheung, Min Li, Michael Otto
Essential Staphylococcus aureus toxin export system.
Nat Med: 2013, 19(3);364-7
[PubMed:23396209] [WorldCat.org] [DOI] (I p)

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