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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1993 [new locus tag: SACOL_RS10415 ]
- pan locus tag?: SAUPAN004996000
- symbol: SACOL1993
- pan gene symbol?: pmtD
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1993 [new locus tag: SACOL_RS10415 ]
- symbol: SACOL1993
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 2055128..2055868
- length: 741
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3238573 NCBI
- RefSeq: YP_186817 NCBI
- BioCyc: see SACOL_RS10415
- MicrobesOnline: 913471 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721ATGAGAATTTTAAATTTAGTTAAGTATGATTTTTATAGTATATTTAAAAGTCCTTTAACA
TATTTAGCGATACTAGTCGTATCTAGTTTGATTGCAACTCAAAGTATACTTATGGCAAAT
TCGATGGATAACCCGAAACATATTATTGTCTATGGATCTGTATTTGCTGCAGCAAAATGG
TTATTGTTAATAATTGGATTAATGTTTGTTGTTAAGACAATTACGCGTGATTTTTCACAA
GGTACAATTCAACTATATATGAGTAAAGTTAAAACACGCGTTGGATACATTATTTCGAAA
ACAATTTCAATTATTTTAATTTCAATATTATTTGCATTAATTCATTATGTGATTTTGATT
GTTGTGCAGGCATCTAGTAATGGAAAAAATTTGGCGTTTTCTAAATATGTAGATAATTTA
TGGTTCTTCCTAATCTTTTTACTATTCTTTGGCTTGTTTTTATTCTTAATCACACTTGCA
TCACAAAAAACAGCAATGATATTTTCATTAGGTGTATTTTTAGTACTCATTGTACCGTTT
ATTAAACCTTTTATTACATTTATCCCAAGATACGGTGAAAAAGTTTTAGATGCTTTTGAT
TATATCCCTTTTGCTTACTTAACTGATAAAATGATTAGCTCTAACTTTGATTTTAGCAAT
TGGCAATGGGTAATTTCATTAGGTTCTATAGTGATATTCTTCATTTTGAATATCTTATAT
GTCGCTAAAAAAGACATTTAA60
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741
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1993 [new locus tag: SACOL_RS10415 ]
- symbol: SACOL1993
- description: hypothetical protein
- length: 246
- theoretical pI: 10.1289
- theoretical MW: 28138.8
- GRAVY: 1.00772
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED :
- FIG01108144: hypothetical protein
- PFAM: ABC-2 (CL0181) ABC2_membrane_2; ABC-2 family transporter protein (PF12679; HMM-score: 37.4)and 2 moreABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 21.9)ABC2_membrane_4; ABC-2 family transporter protein (PF12730; HMM-score: 19.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 6
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0
- N-terminally Anchored Score: 0
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.070487
- TAT(Tat/SPI): 0.000757
- LIPO(Sec/SPII): 0.043602
- predicted transmembrane helices (TMHMM): 6
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDNPKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTISIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLASQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDFSNWQWVISLGSIVIFFILNILYVAKKDI
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Integral membrane [1] [2] [3]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: PmtR* (repression) regulon
PmtR* (TF) important in Hypothetical ABC transporter; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]
Som S Chatterjee, Hwang-Soo Joo, Anthony C Duong, Thomas D Dieringer, Vee Y Tan, Yan Song, Elizabeth R Fischer, Gordon Y C Cheung, Min Li, Michael Otto
Essential Staphylococcus aureus toxin export system.
Nat Med: 2013, 19(3);364-7
[PubMed:23396209] [WorldCat.org] [DOI] (I p)