PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1868 [new locus tag: NWMN_RS10735 ]
• pan locus tag^?: SAUPAN004998000
• symbol: NWMN_1868
• pan gene symbol^?: pmtB
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1868 [new locus tag: NWMN_RS10735 ]
• symbol: NWMN_1868
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2081061..2081741
• length: 681
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331137 NCBI
• RefSeq: YP_001332902 NCBI
• BioCyc:
• MicrobesOnline: 3707456 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  661
  
  ATGAAGGCATTGTTAATTAGGAATTTTAAATTACGACGATATACACTTATCATCTATGTT
  CTATTATTAACCTTATATCCTTTTTATATAATGCTTGACTCAACAAAGTTCTTTTACTTA
  TTGCAATCTTTTATAAGTCCAACAATATTGATTATTTGGATTTTAGATGCTGGTCACTTA
  TTTAGACTCAATCGTAGATTAGGTGGTAATGATTCATATTATTTTTATATGAGTTTACCA
  GTGTCAAAAAAACAATTGCTTAATGCCAACTATATTACATGCATTGTTTTAACATTAATT
  GGAACGCTTGTTATTAGTTTATATGCTTATGAAGCAGATGTGATTGAACCAAATTCAATC
  TATTTTTCAACTGCATATGCATTTGTCATATCTAACTTCTTGTCTATACCAATTGCATTT
  AGTCAATTTACAGAATTGCGTAGAGTCAAAGTGCCATATGGTATATACGTGTTTACTATT
  ATCATTTTAGTTCCATTTTTATTTTCAATTGCAATAGTATTGGTGAATTATTTTGTTCTA
  AGTCAATCATCATTCCCAGATTTATATTCATATATTTTAAATATTGGTTTTCTAATTATA
  AGCATTGTTATACTTATTGTTAATTATTTTAAACAGCTCAATAAAATAAATACTAGAAAA
  TTTAAAGGAGGCAGTCGATGA
  60
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  681
  

⊟Protein

⊟General

• locus tag: NWMN_1868 [new locus tag: NWMN_RS10735 ]
• symbol: NWMN_1868
• description: hypothetical protein
• length: 226
• theoretical pI: 10.0187
• theoretical MW: 26293.2
• GRAVY: 0.784956

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  ABC-2 (CL0181) ABC2_membrane_5; ABC-2 family transporter protein (PF13346; HMM-score: 94.5)
  and 2 more
  ABC2_membrane_2; ABC-2 family transporter protein (PF12679; HMM-score: 15)
  no clan defined DUF441; Protein of unknown function (DUF441) (PF04284; HMM-score: 7.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002953
  • TAT(Tat/SPI): 0.000125
  • LIPO(Sec/SPII): 0.001661
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 151222080 NCBI
• RefSeq: YP_001332902 NCBI
• UniProt: A0A0H3KDV6 UniProt

⊟Protein sequence

• MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSFISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGTLVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTIIILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINTRKFKGGSR

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1866 < NWMN_1867 < NWMN_1868 < NWMN_1869 < NWMN_1870

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Som S Chatterjee, Hwang-Soo Joo, Anthony C Duong, Thomas D Dieringer, Vee Y Tan, Yan Song, Elizabeth R Fischer, Gordon Y C Cheung, Min Li, Michael Otto
Essential Staphylococcus aureus toxin export system.
Nat Med: 2013, 19(3);364-7
[PubMed:23396209] [WorldCat.org] [DOI] (I p)