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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1943 [new locus tag: SACOL_RS10160 ]
  • pan locus tag?: SAUPAN004900000
  • symbol: vraS
  • pan gene symbol?: vraS
  • synonym:
  • product: sensor histidine kinase VraS

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1943 [new locus tag: SACOL_RS10160 ]
  • symbol: vraS
  • product: sensor histidine kinase VraS
  • replicon: chromosome
  • strand: -
  • coordinates: 2005560..2006603
  • length: 1044
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAACCACTACATTAGAACAATTGGTTCAATGCTCATCTTAGTATATAGCATGCTAGCT
    GCATTTCTGTTCATCGATAAAGTTTTTGTAAATATCATCTATTTTCAAGGTATGTTTTAT
    ACACAAATATTCGGAATACCAGTTTTTTTATTTTTAAATCTCATCATCATATTATTGTGT
    ATTATTGTTGGTTCGGTACTCGCTTACAAAATCAATCAGCAAAATGATTGGATTAAGACG
    CAAATTGAGCGTTCAATGGAAGGCGAAACAGTTGGCATTAATGATCAAAATATAGAAATA
    TATAGTGAAACGTTAGATTTATACCATACACTCGTACCTTTAAATCAAGAATTGCATAAG
    TTGCGACTTAAAACTCAAAACTTAACCAATGAAAATTATAATATTAATGATGTGAAAGTT
    AAAAAGATTATTGAAGATGAACGTCAAAGACTAGCACGAGAACTTCACGATTCTGTTAGT
    CAGCAACTTTTTGCGGCAAGTATGATGCTATCTGCTATCAAAGAAACGAAGTTAGAACCA
    CCATTAGACCAACAAATTCCTATTTTAGAGAAAATGGTTCAAGATTCGCAGTTAGAAATG
    CGTGCTTTGCTGTTACATTTAAGACCGCTTGGTTTAAAAGACAAATCTTTAGGTGAGGGT
    ATTAAAGATTTAGTTATTGATTTACAAAAAAAAGTGCCAATGAAAGTTGTGCATGAAATA
    CAAGATTTTAAAGTGCCTAAAGGTATTGAAGATCATTTGTTCAGAATTACACAGGAAGCA
    ATTTCGAATACATTGCGTCATTCAAACGGTACAAAAGTGACAGTAGAATTGTTTAATAAA
    GACGATTATTTATTGTTGAGAATTCAAGATAATGGTAAAGGTTTTAATGTTGATGAAAAA
    TTAGAACAAAGTTATGGACTTAAAAATATGCGTGAAAGAGCTTTGGAAATTGGTGCAACG
    TTCCATATTGTATCATTGCCAGATTCAGGTACACGTATCGAGGTGAAAGCACCTTTAAAT
    AAGGAGGATTCGTATGACGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1044

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1943 [new locus tag: SACOL_RS10160 ]
  • symbol: VraS
  • description: sensor histidine kinase VraS
  • length: 347
  • theoretical pI: 6.06404
  • theoretical MW: 40044.2
  • GRAVY: -0.145533

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 17.5)
  • TheSEED  :
    • Cell envelope stress response system LiaFSR, sensor histidine kinase LiaS(VraS)
    CBSS-176280.1.peg.1561  Sensor histidine kinase VraS
  • PFAM:
    His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 60.1)
    and 2 more
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 33.2)
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 17.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002505
    • TAT(Tat/SPI): 0.000203
    • LIPO(Sec/SPII): 0.058369
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIFGIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYSETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVSQQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLGEGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVELFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIEVKAPLNKEDSYDD

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

S Gardete, S W Wu, S Gill, A Tomasz
Role of VraSR in antibiotic resistance and antibiotic-induced stress response in Staphylococcus aureus.
Antimicrob Agents Chemother: 2006, 50(10);3424-34
[PubMed:17005825] [WorldCat.org] [DOI] (P p)Benjamin P Howden, Danielle J Smith, Ashley Mansell, Paul D R Johnson, Peter B Ward, Timothy P Stinear, John K Davies
Different bacterial gene expression patterns and attenuated host immune responses are associated with the evolution of low-level vancomycin resistance during persistent methicillin-resistant Staphylococcus aureus bacteraemia.
BMC Microbiol: 2008, 8;39
[PubMed:18304359] [WorldCat.org] [DOI] (I e)Elena Galbusera, Adriana Renzoni, Diego O Andrey, Antoinette Monod, Christine Barras, Paolo Tortora, Alessandra Polissi, William L Kelley
Site-specific mutation of Staphylococcus aureus VraS reveals a crucial role for the VraR-VraS sensor in the emergence of glycopeptide resistance.
Antimicrob Agents Chemother: 2011, 55(3);1008-20
[PubMed:21173175] [WorldCat.org] [DOI] (I p)Susana Gardete, Choonkeun Kim, Boris M Hartmann, Michael Mwangi, Christelle M Roux, Paul M Dunman, Henry F Chambers, Alexander Tomasz
Genetic pathway in acquisition and loss of vancomycin resistance in a methicillin resistant Staphylococcus aureus (MRSA) strain of clonal type USA300.
PLoS Pathog: 2012, 8(2);e1002505
[PubMed:22319446] [WorldCat.org] [DOI] (I p)

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