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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1866 [new locus tag: SAUSA300_RS10190 ]
  • pan locus tag?: SAUPAN004900000
  • symbol: vraS
  • pan gene symbol?: vraS
  • synonym:
  • product: two-component sensor histidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1866 [new locus tag: SAUSA300_RS10190 ]
  • symbol: vraS
  • product: two-component sensor histidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2026206..2027249
  • length: 1044
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAACCACTACATTAGAACAATTGGTTCAATGCTCATCTTAGTATATAGCATGCTAGCT
    GCATTTCTGTTCATCGATAAAGTTTTTGTAAATATCATCTATTTTCAAGGTATGTTTTAT
    ACACAAATATTCGGAATACCAGTTTTTTTATTTTTAAATCTCATCATCATATTATTGTGT
    ATTATTGTTGGTTCGGTACTCGCTTACAAAATCAATCAGCAAAATGATTGGATTAAGACG
    CAAATTGAGCGTTCAATGGAAGGCGAAACAGTTGGCATTAATGATCAAAATATAGAAATA
    TATAGTGAAACGTTAGATTTATACCATACACTCGTACCTTTAAATCAAGAATTGCATAAG
    TTGCGACTTAAAACTCAAAACTTAACCAATGAAAATTATAATATTAATGATGTGAAAGTT
    AAAAAGATTATTGAAGATGAACGTCAAAGACTAGCACGAGAACTTCACGATTCTGTTAGT
    CAGCAACTTTTTGCGGCAAGTATGATGCTATCTGCTATCAAAGAAACGAAGTTAGAACCA
    CCATTAGACCAACAAATTCCTATTTTAGAGAAAATGGTTCAAGATTCGCAGTTAGAAATG
    CGTGCTTTGCTGTTACATTTAAGACCGCTTGGTTTAAAAGACAAATCTTTAGGTGAGGGT
    ATTAAAGATTTAGTTATTGATTTACAAAAAAAAGTGCCAATGAAAGTTGTGCATGAAATA
    CAAGATTTTAAAGTGCCTAAAGGTATTGAAGATCATTTGTTCAGAATTACACAGGAAGCA
    ATTTCGAATACATTGCGTCATTCAAACGGTACAAAAGTGACAGTAGAATTGTTTAATAAA
    GACGATTATTTATTGTTGAGAATTCAAGATAATGGTAAAGGTTTTAATGTTGATGAAAAA
    TTAGAACAAAGTTATGGACTTAAAAATATGCGTGAAAGAGCTTTGGAAATTGGTGCAACG
    TTCCATATTGTATCATTGCCAGATTCAGGTACACGTATCGAGGTGAAAGCACCTTTAAAT
    AAGGAGGATTCGTATGACGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1044

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1866 [new locus tag: SAUSA300_RS10190 ]
  • symbol: VraS
  • description: two-component sensor histidine kinase
  • length: 347
  • theoretical pI: 6.06404
  • theoretical MW: 40044.2
  • GRAVY: -0.145533

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
    EC 2.7.3.-?  ExPASy
  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 17.5)
  • TheSEED  :
    • Cell envelope stress response system LiaFSR, sensor histidine kinase LiaS(VraS)
    CBSS-176280.1.peg.1561  Sensor histidine kinase VraS
  • PFAM:
    His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 60.1)
    and 2 more
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 33.2)
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 17.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002505
    • TAT(Tat/SPI): 0.000203
    • LIPO(Sec/SPII): 0.058369
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIFGIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYSETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVSQQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLGEGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVELFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIEVKAPLNKEDSYDD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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