Jump to navigation
Jump to search
NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_02099
- pan locus tag?: SAUPAN004900000
- symbol: SAOUHSC_02099
- pan gene symbol?: vraS
- synonym:
- product: histidine kinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_02099
- symbol: SAOUHSC_02099
- product: histidine kinase
- replicon: chromosome
- strand: -
- coordinates: 1973581..1974624
- length: 1044
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3921171 NCBI
- RefSeq: YP_500590 NCBI
- BioCyc: G1I0R-1987 BioCyc
- MicrobesOnline: 1290546 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021ATGAACCACTACATTAGAACAATTGGTTCAATGCTCATCTTAGTATATAGCATGCTAGCT
GCATTTCTGTTCATCGATAAAGTTTTTGTAAATATCATCTATTTTCAAGGTATGTTTTAT
ACACAAATATTCGGAATACCAGTTTTTTTATTTTTAAATCTCATCATCATATTATTGTGT
ATTATTGTTGGTTCGGTACTCGCTTACAAAATCAATCAGCAAAATGATTGGATTAAGACG
CAAATTGAGCGTTCAATGGAAGGCGAAACAGTTGGCATTAATGATCAAAATATAGAAATA
TATAGTGAAACGTTAGATTTATACCATACACTCGTACCTTTAAATCAAGAATTGCATAAG
TTGCGACTTAAAACTCAAAACTTAACCAATGAAAATTATAATATTAATGATGTGAAAGTT
AAAAAGATTATTGAAGATGAACGTCAAAGACTAGCACGAGAACTTCACGATTCTGTTAGT
CAGCAACTTTTTGCGGCAAGTATGATGCTATCTGCTATCAAAGAAACGAAGTTAGAACCA
CCATTAGACCAACAAATTCCTATTTTAGAGAAAATGGTTCAAGATTCGCAGTTAGAAATG
CGTGCTTTGCTGTTACATTTAAGACCGCTTGGTTTAAAAGACAAATCTTTAGGTGAGGGT
ATTAAAGATTTAGTTATTGATTTACAAAAAAAAGTGCCAATGAAAGTTGTGCATGAAATA
CAAGATTTTAAAGTGCCTAAAGGTATTGAAGATCATTTGTTCAGAATTACACAGGAAGCA
ATTTCGAATACATTGCGTCATTCAAACGGTACAAAAGTGACAGTAGAATTGTTTAATAAA
GACGATTATTTATTGTTGAGAATTCAAGATAATGGTAAAGGTTTTAATGTTGATGAAAAA
TTAGAACAAAGTTATGGACTTAAAAATATGCGTGAAAGAGCTTTGGAAATTGGTGCAACG
TTCCATATTGTATCATTGCCAGATTCAGGTACACGTATCGAGGTGAAAGCACCTTTAAAT
AAGGAGGATTCGTATGACGATTAA60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
1020
1044
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_02099
- symbol: SAOUHSC_02099
- description: histidine kinase
- length: 347
- theoretical pI: 6.06404
- theoretical MW: 40044.2
- GRAVY: -0.145533
⊟Function[edit | edit source]
- reaction: EC 2.7.13.3? ExPASyHistidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidineEC 2.7.3.-? ExPASy
- TIGRFAM: Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 17.5)
- TheSEED :
- Cell envelope stress response system LiaFSR, sensor histidine kinase LiaS(VraS)
- PFAM: His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 60.1)and 2 moreHATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 33.2)HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 17.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0.17
- Cytoplasmic Membrane Score: 9.51
- Cellwall Score: 0.16
- Extracellular Score: 0.15
- Internal Helices: 2
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002505
- TAT(Tat/SPI): 0.000203
- LIPO(Sec/SPII): 0.058369
- predicted transmembrane helices (TMHMM): 2
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIFGIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYSETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVSQQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLGEGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVELFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIEVKAPLNKEDSYDD
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_02098 < SAOUHSC_02099 < SAOUHSC_02100 < SAOUHSC_02101predicted SigA promoter [3] : S823 < SAOUHSC_02098 < SAOUHSC_02099 < SAOUHSC_02100 < SAOUHSC_02101 < S824 < SAOUHSC_02102predicted SigA promoter [3] : SAOUHSC_02098 < SAOUHSC_02099 < SAOUHSC_02100 < SAOUHSC_02101 < S824 < SAOUHSC_02102 < SAOUHSC_A02013 < S826
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)