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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1747 [new locus tag: SACOL_RS08940 ]
  • pan locus tag?: SAUPAN004347000
  • symbol: accA
  • pan gene symbol?: accA
  • synonym:
  • product: acetyl-CoA carboxylase carboxyltransferase subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1747 [new locus tag: SACOL_RS08940 ]
  • symbol: accA
  • product: acetyl-CoA carboxylase carboxyltransferase subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 1784942..1785886
  • length: 945
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTAGATTTTGAAAAACCACTTTTTGAAATTCGAAATAAAATTGAATCTTTAAAAGAA
    TCTCAAGATAAAAATGATGTGGATTTACAAGAAGAAATTGACATGCTTGAAGCGTCATTG
    GAACGAGAAACTAAAAAAATATATACAAATCTAAAACCATGGGATCGTGTGCAAATTGCG
    CGTTTGCAAGAAAGACCTACGACCCTAGATTATATTCCATATATCTTTGATTCGTTTATG
    GAACTACATGGTGATCGTAATTTTAGAGATGATCCAGCAATGATTGGTGGTATTGGCTTT
    TTAAATGGTCGTGCTGTTACAGTTATTGGACAACAACGTGGAAAAGATACAAAAGATAAT
    ATTTATCGAAATTTTGGTATGGCGCATCCAGAAGGTTATCGAAAAGCATTACGTTTAATG
    AAACAAGCTGAAAAATTCAATCGTCCTATCTTTACATTTATAGATACAAAAGGTGCATAT
    CCTGGTAAAGCTGCTGAAGAACGTGGACAAAGTGAATCTATCGCAACAAATTTGATTGAG
    ATGGCTTCATTAAAAGTACCAGTTATTGCGATTGTCATTGGTGAAGGTGGCAGTGGAGGT
    GCTCTAGGTATTGGTATTGCCAATAAAGTATTGATGTTAGAGAATAGTACTTACTCTGTT
    ATATCTCCTGAAGGTGCAGCGGCATTATTATGGAAAGACAGTAATTTGGCTAAAATTGCA
    GCTGAAACAATGAAAATTACTGCCCATGATATTAAGCAATTAGGTATTATAGATGATGTC
    ATTTCTGAACCACTTGGCGGTGCACATAAAGATATTGAACAGCAAGCTTTAGCTATTAAA
    TCAGCGTTTGTTGCACAGTTAGATTCACTTGAGTCATTATCACGTGATGAAATTGCTAAT
    GATCGCTTTGAAAAATTCAGAAATATCGGTTCTTATATAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    945

⊟Protein[edit | edit source]

Protein Data Bank: 2F9I
Protein Data Bank: 5KDR

⊟General[edit | edit source]

  • locus tag: SACOL1747 [new locus tag: SACOL_RS08940 ]
  • symbol: AccA
  • description: acetyl-CoA carboxylase carboxyltransferase subunit alpha
  • length: 314
  • theoretical pI: 4.95687
  • theoretical MW: 35069.7
  • GRAVY: -0.340764

⊟Function[edit | edit source]

  • reaction:
    EC 6.4.1.2?  ExPASy
    Acetyl-CoA carboxylase ATP + acetyl-CoA + HCO3(-) = ADP + phosphate + malonyl-CoA
  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, carboxyl transferase, alpha subunit (TIGR00513; EC 6.4.1.2; HMM-score: 486.1)
    and 4 more
    biotin-independent malonate decarboxylase, gamma subunit (TIGR03134; EC 4.1.1.88; HMM-score: 45.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds methylmalonyl-CoA decarboxylase alpha subunit (TIGR01117; EC 4.1.1.41; HMM-score: 29.6)
    Metabolism Energy metabolism Fermentation methylmalonyl-CoA decarboxylase alpha subunit (TIGR01117; EC 4.1.1.41; HMM-score: 29.6)
    Metabolism Energy metabolism ATP-proton motive force interconversion methylmalonyl-CoA decarboxylase alpha subunit (TIGR01117; EC 4.1.1.41; HMM-score: 29.6)
  • TheSEED  :
    • Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2)
    Fatty Acids, Lipids, and Isoprenoids Fatty acids Fatty Acid Biosynthesis FASII  Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2)
  • PFAM:
    ClpP_crotonase (CL0127) ACCA; Acetyl co-enzyme A carboxylase carboxyltransferase alpha subunit (PF03255; HMM-score: 211.4)
    and 3 more
    Carboxyl_trans; Carboxyl transferase domain (PF01039; HMM-score: 55.1)
    MdcE; Malonate decarboxylase gamma subunit (MdcE) (PF06833; HMM-score: 18.7)
    ECH_1; Enoyl-CoA hydratase/isomerase (PF00378; HMM-score: 17)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001877
    • TAT(Tat/SPI): 0.000609
    • LIPO(Sec/SPII): 0.000422
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERETKKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLNGRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAYPGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTYSVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQALAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 376 [4]
  • interaction partners:
    SACOL1748(accD)acetyl-CoA carboxylase subunit beta  [5] (data from MRSA252)
    SACOL1760(ackA)acetate kinase  [5] (data from MRSA252)
    SACOL2657(arcA)arginine deiminase  [5] (data from MRSA252)
    SACOL0842(eno)phosphopyruvate hydratase  [5] (data from MRSA252)
    SACOL1199(ftsZ)cell division protein FtsZ  [5] (data from MRSA252)
    SACOL0593(fusA)elongation factor G  [5] (data from MRSA252)
    SACOL0838(gapA1)glyceraldehyde 3-phosphate dehydrogenase  [5] (data from MRSA252)
    SACOL1513(hup)DNA-binding protein HU  [5] (data from MRSA252)
    SACOL1741(icd)isocitrate dehydrogenase  [5] (data from MRSA252)
    SACOL2623(mqo2)malate:quinone oxidoreductase  [5] (data from MRSA252)
    SACOL1102(pdhA)pyruvate dehydrogenase complex E1 component subunit alpha  [5] (data from MRSA252)
    SACOL1103(pdhB)pyruvate dehydrogenase complex E1 component subunit beta  [5] (data from MRSA252)
    SACOL1104(pdhC)branched-chain alpha-keto acid dehydrogenase E2  [5] (data from MRSA252)
    SACOL1105(pdhD)dihydrolipoamide dehydrogenase  [5] (data from MRSA252)
    SACOL2128(pdp)pyrimidine-nucleoside phosphorylase  [5] (data from MRSA252)
    SACOL0584(rplA)50S ribosomal protein L1  [5] (data from MRSA252)
    SACOL2236(rplB)50S ribosomal protein L2  [5] (data from MRSA252)
    SACOL2239(rplC)50S ribosomal protein L3  [5] (data from MRSA252)
    SACOL2238(rplD)50S ribosomal protein L4  [5] (data from MRSA252)
    SACOL2224(rplF)50S ribosomal protein L6  [5] (data from MRSA252)
    SACOL0585(rplJ)50S ribosomal protein L10  [5] (data from MRSA252)
    SACOL2220(rplO)50S ribosomal protein L15  [5] (data from MRSA252)
    SACOL1257(rplS)50S ribosomal protein L19  [5] (data from MRSA252)
    SACOL1274(rpsB)30S ribosomal protein S2  [5] (data from MRSA252)
    SACOL2233(rpsC)30S ribosomal protein S3  [5] (data from MRSA252)
    SACOL1769(rpsD)30S ribosomal protein S4  [5] (data from MRSA252)
    SACOL2222(rpsE)30S ribosomal protein S5  [5] (data from MRSA252)
    SACOL2206(rpsI)30S ribosomal protein S9  [5] (data from MRSA252)
    SACOL2214(rpsK)30S ribosomal protein S11  [5] (data from MRSA252)
    SACOL0594(tuf)elongation factor Tu  [5] (data from MRSA252)
    SACOL0731LysR family transcriptional regulator  [5] (data from MRSA252)
    SACOL1759universal stress protein  [5] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 41.21 h [6]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 5.13 5.14 5.15 5.16 5.17 5.18 5.19 5.20 5.21 5.22 5.23 5.24 5.25 5.26 5.27 5.28 5.29 5.30 5.31 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  6. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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