From AureoWiki

Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL

locus tag: SACOL0837 [new locus tag: SACOL_RS04305 ]

pan locus tag^?: SAUPAN002705000

symbol: gapR

pan gene symbol^?: gapR

synonym:
product: glycolytic operon regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL0837 [new locus tag: SACOL_RS04305 ]
symbol: gapR
product: glycolytic operon regulator
replicon: chromosome
strand: +
coordinates: 863776..864789
length: 1014
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID: 3238376 NCBI
RefSeq: YP_185711 NCBI
BioCyc: see SACOL_RS04305
MicrobesOnline: 912310 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
GTGAAAGACTTATTGCAAGCACAGCAAAAGCTTATACCGGATCTCATAGATAAAATGTAT
AAACGTTTTTCTATTCTTACTACTATCTCAAAAAATCAGCCTGTCGGACGTCGAAGTTTA
AGCGAACATATGGATATGACTGAACGTGTACTGCGTTCTGAAACAGATATGCTTAAGAAA
CAAGATTTGATAAAAGTTAAGCCTACCGGAATGGAAATTACAGCTGAAGGTGAGCAACTG
ATTTCGCAATTGAAAGGTTACTTTGATATCTATGCAGATGATAATCGTCTGTCAGAAGGT
ATTAAGAATAAATTTCAAATTAAGGAAGTTCATGTTGTTCCTGGTGATGCTGATAATAGT
CAATCTGTTAAAACAGAATTAGGTAGACAAGCAGGTCAATTACTTGAAGGCATATTACAA
GAAGACGCGATAGTTGCTGTAACTGGCGGATCCACGATGGCATGTGTTAGTGAAGCAATT
CATTTATTACCATATAATGTATTCTTCGTACCAGCCAGAGGTGGACTAGGCGAAAATGTT
GTCTTTCAGGCAAACACAATTGCAGCCAGTATGGCACAACAAGCTGGCGGTTATTATACG
ACGATGTATGTACCTGATAATGTCAGTGAAACAACATATAATACATTGTTGTTAGAGCCA
TCAGTCATAAACACTTTAGACAAAATTAAACAAGCAAACGTTATATTACACGGCATTGGT
GATGCGCTGAAGATGGCGCATCGACGTCAATCACCTGAAAAGGTCATTGAACAACTTCAA
CATCATCAAGCTGTCGGAGAGGCATTTGGTTATTATTTTGATACACAAGGTCAAATTGTC
CATAAGGTTAAAACAATTGGACTTCAATTAGAAGACCTTGAATCAAAAGACTTTATTTTT
GCAGTTGCAGGAGGCAAATCGAAAGGTGAAGCAATTAAAGCATACTTGACGATTGCACCC
AAGAATACAGTGTTAATCACTGATGAAGCCGCAGCAAAGATAATACTTGAATAA
60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
1014

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SACOL0837 [new locus tag: SACOL_RS04305 ]
symbol: GapR
description: glycolytic operon regulator
length: 337
theoretical pI: 6.11378
theoretical MW: 37210.4
GRAVY: -0.196736

⊟Function[edit | edit source]

TIGRFAM:
Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD family protein (TIGR04548; HMM-score: 16.8)
TheSEED :
- Central glycolytic genes regulator
⊞PFAM:
NADP_Rossmann (CL0063) Sugar-bind; Putative sugar-binding domain (PF04198; HMM-score: 263.5)
and 4 more
HTH (CL0123) CggR_N; CggR N-terminal DNA binding domain (PF21715; HMM-score: 94.9)
PH0730-like_N; PH0730-like, N-terminal domain (PF22167; HMM-score: 18.8)
HTH_45; Winged helix-turn-helix (PF14947; HMM-score: 16.9)
MotA_activ; Transcription factor MotA, activation domain (PF09114; HMM-score: 15.9)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effector: Fructose-1,6-bisphosphate
⊞genes regulated by GapR, TF important in GlycolysisRegPrecise
repression
gapR > gapA1 > pgk > tpiA > pgm > eno

⊟Localization[edit | edit source]

⊞
PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
⊞
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9945
- Cytoplasmic Membrane Score: 0.005
- Cell wall & surface Score: 0.0001
- Extracellular Score: 0.0004
⊞
LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002249
- TAT(Tat/SPI): 0.000242
- LIPO(Sec/SPII): 0.000544
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 57650113 NCBI
RefSeq: YP_185711 NCBI
UniProt: A0A0H2WW37 UniProt

⊟Protein sequence[edit | edit source]

MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHMDMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIKNKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAIHLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLLEPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQGQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKIILE

⊟Experimental data[edit | edit source]

experimentally validated: PeptideAtlas
protein localization: Cytoplasmic ^[1] ^[2] ^[3]
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: gapR > gapA1 > pgk > tpiA > pgm > eno

⊟Regulation[edit | edit source]

⊞regulator: GapR (repression) regulon
GapR (TF) important in Glycolysis; RegPrecise

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib:

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e)
↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p)
↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL0837&oldid=81345"