From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0740 [new locus tag: NWMN_RS04190 ]
  • pan locus tag?: SAUPAN002705000
  • symbol: gapR
  • pan gene symbol?: gapR
  • synonym:
  • product: glycolytic operon regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0740 [new locus tag: NWMN_RS04190 ]
  • symbol: gapR
  • product: glycolytic operon regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 832890..833903
  • length: 1014
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGAAAGACTTATTGCAAGCACAGCAAAAGCTTATACCGGATCTCATAGATAAAATGTAT
    AAACGTTTTTCTATTCTTACTACTATCTCAAAAAATCAGCCTGTCGGACGTCGAAGTTTA
    AGCGAACATATGGATATGACTGAACGTGTACTGCGTTCTGAAACAGATATGCTTAAGAAA
    CAAGATTTGATAAAAGTTAAGCCTACCGGAATGGAAATTACAGCTGAAGGTGAGCAACTG
    ATTTCGCAATTGAAAGGTTACTTTGATATCTATGCAGATGATAATCGTCTGTCAGAAGGT
    ATTAAGAATAAATTTCAAATTAAGGAAGTTCATGTTGTTCCTGGTGATGCTGATAATAGT
    CAATCTGTTAAAACAGAATTAGGTAGACAAGCAGGTCAATTACTTGAAGGCATATTACAA
    GAAGACGCGATAGTTGCTGTAACTGGCGGATCCACGATGGCATGTGTTAGTGAAGCAATT
    CATTTATTACCATATAATGTATTCTTCGTACCAGCCAGAGGTGGACTAGGCGAAAATGTT
    GTCTTTCAGGCAAACACAATTGCAGCCAGTATGGCACAACAAGCTGGCGGTTATTATACG
    ACGATGTATGTACCTGATAATGTCAGTGAAACAACATATAATACATTGTTGTTAGAGCCA
    TCAGTCATAAACACTTTAGACAAAATTAAACAAGCAAACGTTATATTACACGGCATTGGT
    GATGCGCTGAAGATGGCGCATCGACGTCAATCACCTGAAAAGGTCATTGAACAACTTCAA
    CATCATCAAGCTGTCGGAGAGGCATTTGGTTATTATTTTGATACACAAGGTCAAATTGTC
    CATAAGGTTAAAACAATTGGACTTCAATTAGAAGACCTTGAATCAAAAGACTTTATTTTT
    GCAGTTGCAGGAGGCAAATCGAAAGGTGAAGCAATTAAAGCATACTTGACGATTGCACCC
    AAGAATACAGTGTTAATCACTGATGAAGCCGCAGCAAAGATAATACTTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1014

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0740 [new locus tag: NWMN_RS04190 ]
  • symbol: GapR
  • description: glycolytic operon regulator
  • length: 337
  • theoretical pI: 6.11378
  • theoretical MW: 37210.4
  • GRAVY: -0.196736

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD family protein (TIGR04548; HMM-score: 16.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    ISOCOT_Fold (CL0246) Sugar-bind; Putative sugar-binding domain (PF04198; HMM-score: 248.2)
    and 2 more
    HTH (CL0123) MotA_activ; Transcription factor MotA, activation domain (PF09114; HMM-score: 15.9)
    HTH_45; Winged helix-turn-helix (PF14947; HMM-score: 15.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Fructose-1,6-bisphosphate
  • genes regulated by GapR, TF important in GlycolysisRegPrecise
    repression
    gapR > gapA > pgk > tpiA > pgm > eno
    transcription unit transferred from N315 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002249
    • TAT(Tat/SPI): 0.000242
    • LIPO(Sec/SPII): 0.000544
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHMDMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIKNKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAIHLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLLEPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQGQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKIILE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: GapR (repression) regulon, SigB (activation) regulon
    GapR(TF)important in Glycolysis; RegPrecise 
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_0740&oldid=56677"