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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2395 [new locus tag: SA_RS13705 ]
  • pan locus tag?: SAUPAN006297000
  • symbol: SA2395
  • pan gene symbol?: ldh2
  • synonym:
  • product: L-lactate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2395 [new locus tag: SA_RS13705 ]
  • symbol: SA2395
  • product: L-lactate dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2682699..2683658
  • length: 960
  • essential: yes DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAACATTTGGTAAAAAGGTTGTATTAATCGGAGATGGATCTGTAGGATCAAGCTAT
    GCCTTTGCAATGGTTACGCAAGGTGTTGCTGATGAATTTGTAATTATTGACATTGCAAAA
    GACAAAGTAAAAGCAGATGTTCAAGATTTAAACCATGGTACAGTCCACAGTCCTTCACCA
    GTTGATGTGAAAGCAGGTGAATACGAAGACTGTAAAGATGCAGATTTAGTTGTTATTACA
    GCTGGTGCACCTCAAAAGCCAGGTGAAACACGTTTACAATTAGTTGAAAAAAATACTAAG
    ATTATGAAGAGCATTGTTAAGAGTGTTATGGATAGCGGCTTTGATGGATATTTCTTAATC
    GCGGCAAACCCTGTTGACATTTTAACAAGATTTGTAAAAGAATATACTGGTTTACCAGCA
    GAGCGTGTTATCGGTTCAGGTACTGTATTGGACAGTGCACGTTTACAATATTTAATTAGC
    CAAGAACTTGGTGTTGCACCTTCAAGTGTTGACGCTAGTATTATTGGTGAGCATGGTGAT
    ACTGAACTTGCAGTTTGGTCACAAGCAAATGTAGCAGGTATTTCAGTATATGACACATTA
    AAAGAACAAACTGGTAGCGAAGCTAAAGCGGAAGAAATTTATGTAAATACACGTGACGCT
    GCTTATGAAATTATCCAAGCTAAAGGGTCAACATACTATGGAATTGCATTAGCATTGATG
    CGCATTTCAAAAGCCATTTTAAATAATGAAAATAATGTCTTAAATGTTTCTATACAATTA
    GATGGTCAATATGGTGGTCACAAAGGCGTTTACCTAGGTGTACCAACATTAGTTAACCAA
    CATGGCGCAGTTAAAATTTATGAAATGCCATTAAGTGCCGAAGAACAAGCGTTGTTCGAT
    AAATCTGTTAAAATATTAGAAGATACATTTGATTCAATTAAATATTTATTAGAAGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2395 [new locus tag: SA_RS13705 ]
  • symbol: SA2395
  • description: L-lactate dehydrogenase
  • length: 319
  • theoretical pI: 4.53599
  • theoretical MW: 34431.8
  • GRAVY: -0.0335423

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.27?  ExPASy
    L-lactate dehydrogenase (S)-lactate + NAD+ = pyruvate + NADH
  • TIGRFAM:
    Metabolism Energy metabolism Anaerobic L-lactate dehydrogenase (TIGR01771; EC 1.1.1.27; HMM-score: 419.8)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis L-lactate dehydrogenase (TIGR01771; EC 1.1.1.27; HMM-score: 419.8)
    and 9 more
    Metabolism Energy metabolism TCA cycle malate dehydrogenase, NAD-dependent (TIGR01763; EC 1.1.1.37; HMM-score: 209.8)
    Metabolism Energy metabolism TCA cycle malate dehydrogenase, NAD-dependent (TIGR01772; EC 1.1.1.37; HMM-score: 71.5)
    malate dehydrogenase (TIGR01759; EC 1.1.1.-; HMM-score: 67.5)
    lactate dehydrogenase (TIGR01756; EC 1.1.1.27; HMM-score: 50.1)
    malate dehydrogenase, NAD-dependent (TIGR01758; EC 1.1.1.37; HMM-score: 46.2)
    malate dehydrogenase, NADP-dependent (TIGR01757; EC 1.1.1.82; HMM-score: 17.7)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 15.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 12.4)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 12.2)
  • TheSEED  :
    • L-lactate dehydrogenase (EC 1.1.1.27)
    Carbohydrates Fermentation Fermentations: Lactate  L-lactate dehydrogenase (EC 1.1.1.27)
    and 1 more
    Carbohydrates Fermentation Fermentations: Mixed acid  L-lactate dehydrogenase (EC 1.1.1.27)
  • PFAM:
    NADP_Rossmann (CL0063) Ldh_1_N; lactate/malate dehydrogenase, NAD binding domain (PF00056; HMM-score: 153.5)
    and 5 more
    LDH_C (CL0341) Ldh_1_C; lactate/malate dehydrogenase, alpha/beta C-terminal domain (PF02866; HMM-score: 121.2)
    NADP_Rossmann (CL0063) Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 16.6)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 15.4)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 15.2)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 14.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.108195
    • TAT(Tat/SPI): 0.002687
    • LIPO(Sec/SPII): 0.004804
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVKADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIMKSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLISQELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTRDAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPTLVNQHGAVKIYEMPLSAEEQALFDKSVKILEDTFDSIKYLLED

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA1984(asp23)alkaline shock protein 23  [1] (data from MRSA252)
    SA1517(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    SA1409(dnaK)molecular chaperone DnaK  [1] (data from MRSA252)
    SA1029(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    SA0505(fus)elongation factor G  [1] (data from MRSA252)
    SA1510(gapB)glyceraldehyde 3-phosphate dehydrogenase 2  [1] (data from MRSA252)
    SA1305(hu)DNA-binding protein II  [1] (data from MRSA252)
    SA0038(mecA)penicillin binding protein 2 prime  [1] (data from MRSA252)
    SA2074(modA)molybdate-binding protein  [1] (data from MRSA252)
    SA1244(odhB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA0944(pdhB)pyruvate dehydrogenase E1 component subunit beta  [1] (data from MRSA252)
    SA0945(pdhC)branched-chain alpha-keto acid dehydrogenase E2 subunit  [1] (data from MRSA252)
    SA0946(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SA0218(pflB)formate acetyltransferase  [1] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [1] (data from MRSA252)
    SA1101(pyrH)uridylate kinase  [1] (data from MRSA252)
    SA0496(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SA2044(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    SA2047(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SA2046(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    SA2035(rplE)50S ribosomal protein L5  [1] (data from MRSA252)
    SA2033(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    SA0497(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    SA2032(rplR)50S ribosomal protein L18  [1] (data from MRSA252)
    SA1502(rplT)50S ribosomal protein L20  [1] (data from MRSA252)
    SA2042(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    SA2045(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    SA1099(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SA2041(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SAS052(rpsD)30S ribosomal protein S4  [1] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SA2016(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    SA2024(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    SA0503(rpsL)30S ribosomal protein S12  [1] (data from MRSA252)
    SA2025(rpsM)30S ribosomal protein S13  [1] (data from MRSA252)
    SA0354(rpsR)30S ribosomal protein S18  [1] (data from MRSA252)
    SA2043(rpsS)30S ribosomal protein S19  [1] (data from MRSA252)
    SA0107(spa)immunoglobulin G binding protein A  [1] (data from MRSA252)
    SA1245(sucA)2-oxoglutarate dehydrogenase E1  [1] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [1] (data from MRSA252)
    SA0182hypothetical protein  [1] (data from MRSA252)
    SA0511hypothetical protein  [1] (data from MRSA252)
    SA0627hypothetical protein  [1] (data from MRSA252)
    SA0802hypothetical protein  [1] (data from MRSA252)
    SA1271threonine dehydratase  [1] (data from MRSA252)
    SA1532hypothetical protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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