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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1411 [new locus tag: SA_RS07965 ]
  • pan locus tag?: SAUPAN004165000
  • symbol: hrcA
  • pan gene symbol?: hrcA
  • synonym:
  • product: heat-inducible transcriptional repressor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1411 [new locus tag: SA_RS07965 ]
  • symbol: hrcA
  • product: heat-inducible transcriptional repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 1616073..1617050
  • length: 978
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTACAGATAGGCAATTGAGTATATTAAACGCAATTGTTGAGGATTATGTTGATTTT
    GGACAACCCGTTGGTTCTAAAACACTAATTGAGCGACATAACTTGAATGTTAGTCCTGCT
    ACAATTAGAAATGAGATGAAACAGCTTGAAGATTTAAACTATATCGAAAAGACACATAGT
    TCTTCAGGGCGTTCGCCATCACAATTAGGTTTTAGGTATTATGTCAATCGTTTACTTGAA
    CAAACATCTCATCAAAAAACAAATAAATTAAGACGATTAAATCAATTGTTAGTTGAGAAT
    CAATATGATGTATCATCAGCATTGACATATTTTGCAGATGAATTATCAAATATATCTCAA
    TATACAACTTTAGTTGTTCATCCTAATCATAAACAAGATATTATCAATAATGTACATTTG
    ATTCGTGCTAATCCTAATTTAGTTATAATGGTTATCGTATTTTCATCAGGTCATGTTGAG
    CATGTACATCTTGCTTCAGACATACCGTTTAGTAATGATAAACTAAATACAATATCAAAT
    TTTGTTACTAACAAACTAACTGAGTTTAATCAGAATTTACAAGATGATATTGTCTCTTTT
    GTTCAATCAGAGCAGGAAGAAATATTTATCAATAAATTGATAAATACGATGAATAATCAT
    ATTTCTAATCAAAGTAATAGCATTTATATGGGTGGAAAGGTTAAACTTATTGATGCATTG
    AATGAAAGTAATGTATCTTCAATTCAGCCCATTCTACAATATATAGAATCAAATAGAATT
    GCAGAATTATTACAAGATATATCTTCACCAAATATTAATGTAAAAATTGGTAATGAAATT
    GATGACAGCTTAAGTGATATTTCAATTGTAACAAGTCAATATCATTTTGATGAAACGTTA
    AAAGGTCAAATCGCTGTGATTGGACCTACAGCTATGCATTATCAAAATGTCATTCAATTA
    TTAAATAGAATTTGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    978

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1411 [new locus tag: SA_RS07965 ]
  • symbol: HrcA
  • description: heat-inducible transcriptional repressor
  • length: 325
  • theoretical pI: 5.71442
  • theoretical MW: 36988.4
  • GRAVY: -0.290769

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions heat-inducible transcription repressor HrcA (TIGR00331; HMM-score: 346.8)
  • TheSEED  :
    • Heat-inducible transcription repressor HrcA
    Stress Response Heat shock Heat shock dnaK gene cluster extended  Heat-inducible transcription repressor HrcA
  • PFAM:
    GAF (CL0161) HrcA; HrcA protein C terminal domain (PF01628; HMM-score: 141.4)
    and 8 more
    HTH (CL0123) HrcA_DNA-bdg; Winged helix-turn-helix transcription repressor, HrcA DNA-binding (PF03444; HMM-score: 18.7)
    no clan defined DUF5764; Family of unknown function (DUF5764) (PF19068; HMM-score: 18.6)
    HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 18.1)
    HTH_11; HTH domain (PF08279; HMM-score: 16.9)
    Tubulin_C (CL0442) FtsZ_C; FtsZ family, C-terminal domain (PF12327; HMM-score: 13.4)
    HTH (CL0123) HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 13.2)
    LexA_DNA_bind; LexA DNA binding domain (PF01726; HMM-score: 12.4)
    Ribosomal_S19e; Ribosomal protein S19e (PF01090; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8738
    • Cytoplasmic Membrane Score: 0.0672
    • Cell wall & surface Score: 0.002
    • Extracellular Score: 0.057
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004618
    • TAT(Tat/SPI): 0.002068
    • LIPO(Sec/SPII): 0.000521
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRNEMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQYDVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVEHVHLASDIPFSNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLINTMNNHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKIGNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CtsR (repression) regulon, HrcA (repression) regulon
    CtsR(TF)important in Heat shock response; RegPrecise 
    HrcA(TF)important in Heat shock response; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Arnaud Chastanet, Juliette Fert, Tarek Msadek
    Comparative genomics reveal novel heat shock regulatory mechanisms in Staphylococcus aureus and other Gram-positive bacteria.
    Mol Microbiol: 2003, 47(4);1061-73
    [PubMed:12581359] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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