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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1836 [new locus tag: SA_RS10535 ]
  • pan locus tag?: SAUPAN005241000
  • symbol: groEL
  • pan gene symbol?: groEL
  • synonym:
  • product: molecular chaperone GroEL

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1836 [new locus tag: SA_RS10535 ]
  • symbol: groEL
  • product: molecular chaperone GroEL
  • replicon: chromosome
  • strand: -
  • coordinates: 2072341..2073957
  • length: 1617
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGTTAAACAATTGAAATTCTCTGAAGATGCACGTCAAGCAATGTTACGTGGTGTAGAC
    CAATTAGCAAATGCAGTTAAAGTAACGATTGGTCCTAAAGGACGTAATGTTGTATTAGAT
    AAAGAGTTTACAGCACCTTTAATTACGAACGATGGTGTAACGATTGCTAAAGAAATCGAA
    TTAGAAGATCCATATGAAAATATGGGGGCTAAACTAGTTCAAGAAGTTGCAAATAAGACA
    AATGAAATTGCTGGTGACGGTACGACAACTGCAACAGTATTAGCTCAAGCAATGATTCAA
    GAAGGCTTGAAAAATGTTACAAGTGGTGCGAACCCAGTTGGTTTACGTCAAGGTATCGAC
    AAAGCAGTTAAAGTTGCTGTAGAAGCATTACATGAAAATTCTCAAAAAGTTGAAAATAAA
    AATGAAATAGCGCAAGTAGGTGCGATTTCAGCAGCAGATGAAGAAATTGGACGTTATATT
    TCTGAAGCTATGGAAAAAGTAGGTAACGATGGTGTCATTACAATTGAAGAATCAAATGGA
    CTAAACACTGAACTAGAAGTGGTTGAAGGTATGCAATTTGATCGTGGTTATCAATCACCA
    TATATGGTTACTGATTCAGATAAAATGGTTGCTGAATTAGAACGCCCATACATTTTAGTA
    ACAGATAAGAAAATCTCGTCTTTCCAAGATATCTTACCTTTATTAGAACAAGTGGTTCAA
    TCTAATCGTCCAATCTTAATTGTAGCTGATGAAGTTGAAGGCGATGCATTAACAAATATC
    GTGCTAAACCGAATGCGTGGCACATTTACAGCTGTTGCAGTAAAGGCGCCTGGTTTCGGT
    GATCGTCGTAAAGCAATGTTAGAAGATTTAGCTATTTTAACTGGTGCGCAAGTGATTACT
    GATGATTTAGGCTTAGATTTAAAAGATGCATCAATTGATATGTTAGGTACTGCAAGTAAA
    GTAGAAGTAACTAAAGATAATACCACTGTTGTTGATGGTGACGGTGACGAAAACAGCATT
    GATGCACGTGTAAGCCAATTGAAATCTCAAATTGAAGAAACTGAATCTGACTTTGATCGT
    GAAAAATTACAAGAGCGCTTAGCTAAATTAGCAGGTGGTGTTGCAGTTATTAAAGTAGGT
    GCAGCAAGTGAAACAGAGCTTAAAGAACGTAAATTACGTATTGAAGATGCATTAAATTCT
    ACACGTGCAGCAGTTGAAGAAGGTATTGTTGCAGGTGGTGGTACTGCATTAGTAAATGTT
    TACCAAAAAGTAAGTGAAATTGAAGCAGAAGGTGACATTGAAACAGGTGTAAATATTGTA
    CTTAAAGCACTAACTGCACCAGTTCGTCAAATTGCTGAAAATGCAGGATTAGAAGGTTCT
    GTTATTGTAGAACGTTTGAAAAACGCAGAGCCGGGTGTTGGTTTTAACGCTGCTACAAAC
    GAGTGGGTTAATATGTTAGAAGCAGGTATCGTTGATCCAACTAAAGTAACACGCTCAGCA
    TTACAACATGCTGCAAGTGTTGCAGCAATGTTCTTAACGACTGAAGCGGTTGTAGCATCA
    ATTCCAGAAAAAAATAATGACCAACCTAACATGGGTGGCATGCCGGGAATGATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1617

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1836 [new locus tag: SA_RS10535 ]
  • symbol: GroEL
  • description: molecular chaperone GroEL
  • length: 538
  • theoretical pI: 4.28546
  • theoretical MW: 57571.8
  • GRAVY: -0.1671

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein folding and stabilization chaperonin GroL (TIGR02348; HMM-score: 825.1)
    and 10 more
    Genetic information processing Protein fate Protein folding and stabilization thermosome, various subunits, archaeal (TIGR02339; HMM-score: 100.4)
    T-complex protein 1, delta subunit (TIGR02342; HMM-score: 89.4)
    T-complex protein 1, eta subunit (TIGR02345; HMM-score: 79.3)
    T-complex protein 1, epsilon subunit (TIGR02343; HMM-score: 74.7)
    T-complex protein 1, zeta subunit (TIGR02347; HMM-score: 71.2)
    T-complex protein 1, beta subunit (TIGR02341; HMM-score: 70.3)
    T-complex protein 1, gamma subunit (TIGR02344; HMM-score: 63.9)
    T-complex protein 1, theta subunit (TIGR02346; HMM-score: 59.7)
    T-complex protein 1, alpha subunit (TIGR02340; HMM-score: 50.1)
    L-seryl-tRNA(Sec) kinase (TIGR03575; EC 2.7.1.164; HMM-score: 15)
  • TheSEED  :
    • Heat shock protein 60 kDa family chaperone GroEL
    Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Heat shock protein 60 family chaperone GroEL
    and 1 more
    Protein Metabolism Protein folding GroEL GroES  Heat shock protein 60 family chaperone GroEL
  • PFAM:
    no clan defined Cpn60_TCP1; TCP-1/cpn60 chaperonin family (PF00118; HMM-score: 334.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004121
    • TAT(Tat/SPI): 0.001123
    • LIPO(Sec/SPII): 0.000853
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFTAPLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEGLKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYISEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYILVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKAPGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVAAMFLTTEAVVASIPEKNNDQPNMGGMPGMM

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA0506(tuf)elongation factor Tu  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CtsR (repression) regulon, HrcA (repression) regulon
    CtsR(TF)important in Heat shock response; RegPrecise 
    HrcA(TF)important in Heat shock response; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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