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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01685
  • pan locus tag?: SAUPAN004165000
  • symbol: SAOUHSC_01685
  • pan gene symbol?: hrcA
  • synonym:
  • product: heat-inducible transcription repressor HrcA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01685
  • symbol: SAOUHSC_01685
  • product: heat-inducible transcription repressor HrcA
  • replicon: chromosome
  • strand: -
  • coordinates: 1595172..1596149
  • length: 978
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTACAGATAGGCAATTGAGTATATTAAACGCAATTGTTGAGGATTATGTTGATTTT
    GGACAACCCGTTGGTTCTAAAACACTAATTGAGCGACATAACTTGAATGTTAGTCCTGCT
    ACAATTAGAAATGAGATGAAACAGCTTGAAGATTTAAACTATATCGAGAAGACACATAGT
    TCTTCAGGGCGTTCGCCATCACAATTAGGTTTTAGGTATTATGTCAATCGTTTACTTGAA
    CAAACATCTCATCAAAAAACAAATAAATTAAGACGATTAAATCAATTGTTAGTTGAGAAT
    CAATATGATGTATCATCAGCATTGACATATTTTGCAGATGAATTATCAAATATATCTCAA
    TATACAACTTTAGTTGTTCATCCTAATCATAAACAAGATATTATCAATAATGTACACTTG
    ATTCGTGCTAATCCTAATTTAGTTATAATGGTTATCGTATTTTCATCAGGTCATGTTGAG
    CATGTACATCTTGCTTCAGACATACCGTTTAATAATGACAAACTAAATACAATATCAAAT
    TTTGTTACAAACAAACTAACTGAGTTTAATCAGAATTTACAAGATGATATTGTCTCTTTT
    GTTCAATCAGAGCAGGAAGAAATTTTTATCAATAAATTGTTAAATACGATGAATAATCAT
    ATTTCTAATCAAAGTAATAGCATTTATATGGGTGGAAAGGTTAAACTTATTGATGCATTG
    AATGAAAGTAATGTATCTTCAATTCAGCCCATTCTACAATATATAGAATCAAATAGAATT
    GCAGAATTATTACAAGATATATCTTCACCAAATATTAATGTAAAAATTGGTAATGAAATT
    GATGACAGCTTAAGTGATATTTCAATTGTAACAAGTCAATATCATTTTGATGAAACGTTA
    AAAGGTCAAATCGCTGTGATTGGACCTACAGCTATGCATTATCAAAATGTCATTCAATTA
    TTAAATAGAATTTGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    978

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01685
  • symbol: SAOUHSC_01685
  • description: heat-inducible transcription repressor HrcA
  • length: 325
  • theoretical pI: 5.71442
  • theoretical MW: 37015.4
  • GRAVY: -0.301231

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions heat-inducible transcription repressor HrcA (TIGR00331; HMM-score: 345.7)
  • TheSEED  :
    • Heat-inducible transcription repressor HrcA
    Stress Response Heat shock Heat shock dnaK gene cluster extended  Heat-inducible transcription repressor HrcA
  • PFAM:
    GAF (CL0161) HrcA; HrcA protein C terminal domain (PF01628; HMM-score: 138.5)
    and 8 more
    HTH (CL0123) HrcA_DNA-bdg; Winged helix-turn-helix transcription repressor, HrcA DNA-binding (PF03444; HMM-score: 18.7)
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 18.2)
    HTH_11; HTH domain (PF08279; HMM-score: 16.7)
    no clan defined FOXO_KIX_bdg; KIX-binding domain of forkhead box O, CR2 (PF16675; HMM-score: 13.3)
    HTH (CL0123) HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 12.5)
    LexA_DNA_bind; LexA DNA binding domain (PF01726; HMM-score: 12.4)
    Tubulin_C (CL0442) FtsZ_C; FtsZ family, C-terminal domain (PF12327; HMM-score: 12.3)
    HTH (CL0123) Ribosomal_S19e; Ribosomal protein S19e (PF01090; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004618
    • TAT(Tat/SPI): 0.002068
    • LIPO(Sec/SPII): 0.000521
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRNEMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQYDVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVEHVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMNNHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKIGNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CtsR* (repression) regulon, HrcA* (repression) regulon
    CtsR*(TF)important in Heat shock response; compare RegPrecise for N315  [3]
    HrcA*(TF)important in Heat shock response; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  4. ↑ Arnaud Chastanet, Juliette Fert, Tarek Msadek
    Comparative genomics reveal novel heat shock regulatory mechanisms in Staphylococcus aureus and other Gram-positive bacteria.
    Mol Microbiol: 2003, 47(4);1061-73
    [PubMed:12581359] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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