PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1256 [new locus tag: SA_RS07125 ]
• pan locus tag^?: SAUPAN003854000
• symbol: SA1256
• pan gene symbol^?: msrB
• synonym:
• product: methionine sulfoxide reductase B

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1256 [new locus tag: SA_RS07125 ]
• symbol: SA1256
• product: methionine sulfoxide reductase B
• replicon: chromosome
• strand: -
• coordinates: 1430012..1430440
• length: 429
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124094 NCBI
• RefSeq: NP_374537 NCBI
• BioCyc: see SA_RS07125
• MicrobesOnline: 103563 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGCTTAAAAAAGATAAAAGTGAACTAACAGACATAGAATATATTGTTACACAAGAAAAT
  GGCACTGAACCACCATTTATGAATGAATATTGGAATCATTTTGCTAAAGGGATTTATGTA
  GATAAGATTTCTGGTAAACCTTTATTTACATCTGAAGAAAAGTTTCATTCTGAATGTGGA
  TGGCCTAGCTTTTCTAAAGCGCTTGATGACGATGAAATTATAGAATTAGTCGACAAATCA
  TTTGGTATGGTAAGAACTGAAGTGCGTTCAGAAGAATCAAATAGTCATTTAGGACACGTC
  TTTAATGATGGACCTAAAGAAAGTGGCGGCTTAAGATACTGTATCAATTCCGCTGCAATT
  CAATTTATTCCATATGAAAAATTAGAAGAATTGGGTTATGGCGATTTAATATCACATTTT
  GATAAGTAG
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  429
  

⊟Protein

⊟General

• locus tag: SA1256 [new locus tag: SA_RS07125 ]
• symbol: SA1256
• description: methionine sulfoxide reductase B
• length: 142
• theoretical pI: 4.49255
• theoretical MW: 16263
• GRAVY: -0.598592

⊟Function

• reaction:
  EC 1.8.4.11?  ExPASy
  Peptide-methionine (S)-S-oxide reductase Peptide-L-methionine + thioredoxin disulfide + H₂O = peptide-L-methionine (S)-S-oxide + thioredoxin L-methionine + thioredoxin disulfide + H₂O = L-methionine (S)-S-oxide + thioredoxin
  EC 1.8.4.12?  ExPASy
  Peptide-methionine (R)-S-oxide reductase Peptide-L-methionine + thioredoxin disulfide + H₂O = peptide-L-methionine (R)-S-oxide + thioredoxin
• TIGRFAM:
  Cellular processes Cellular processes Adaptations to atypical conditions methionine-R-sulfoxide reductase (TIGR00357; EC 1.8.4.-; HMM-score: 192.3)
  Genetic information processing Protein fate Protein modification and repair methionine-R-sulfoxide reductase (TIGR00357; EC 1.8.4.-; HMM-score: 192.3)
• TheSEED  :
  • Peptide-methionine (R)-S-oxide reductase MsrB (EC 1.8.4.12)
  Protein Metabolism Protein processing and modification Peptide methionine sulfoxide reductase  Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12)
• PFAM:
  Beta-tent (CL0080) SelR; SelR domain (PF01641; HMM-score: 159.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.017153
  • TAT(Tat/SPI): 0.00034
  • LIPO(Sec/SPII): 0.00047
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927004 NCBI
• RefSeq: NP_374537 NCBI
• UniProt: P99065 UniProt

⊟Protein sequence

• MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKISGKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFNDGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1254 < SA1255 < SA1256 < SA1257

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)Grazyna Młynarczyk, Andrzej Młynarczyk, Ksenia Szymanek, Mirosław Luczak
[The frequency of the occurrence of genes ermA, ermB, ermC and msrA/B among methicillin-resistant Staphylococcus aureus strains resistant to erythromycin]. [Czestość wystepowania genów ermA, ermB, ermC i msrA/B u metycylino-opornych klinicznych szczepów Staphylococcus aureus opornych na erytromycyne izolowanych w Polsce.]
Med Dosw Mikrobiol: 2006, 58(3);183-90
[PubMed:17340992] [WorldCat.org] (P p)