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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS02940 [old locus tag: NWMN_0505 ]
- pan locus tag?: SAUPAN002315000
- symbol: NWMN_RS02940
- pan gene symbol?: rpoC
- synonym:
- product: DNA-directed RNA polymerase subunit beta'
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS02940 [old locus tag: NWMN_0505 ]
- symbol: NWMN_RS02940
- product: DNA-directed RNA polymerase subunit beta'
- replicon: chromosome
- strand: +
- coordinates: 579792..583388
- length: 3597
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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3541ATGAAAATAGGATTGGCTTCACCTGAAAAAATCCGTTCTTGGTCTTTTGGTGAAGTTAAA
AAACCTGAAACAATCAACTACCGTACATTAAAACCTGAAAAAGATGGTCTATTCTGTGAA
AGAATTTTCGGACCTACAAAAGACTGGGAATGTAGTTGTGGTAAATACAAACGTGTTCGC
TACAAAGGCATGGTCTGTGACAGATGTGGAGTTGAAGTAACTAAATCTAAAGTACGTCGT
GAAAGAATGGGTCACATTGAACTTGCTGCTCCAGTTTCTCACATTTGGTATTTCAAAGGT
ATACCAAGTCGTATGGGATTATTACTTGACATGTCACCAAGAGCATTAGAAGAAGTTATT
TACTTTGCTTCTTATGTTGTTGTAGATCCAGGTCCAACTGGTTTAGAAAAGAAAACTTTA
TTATCTGAAGCTGAATTCAGAGATTATTATGATAAATACCCAGGTCAATTCGTTGCAAAA
ATGGGTGCAGAAGGTATTAAAGATTTACTTGAAGAGATTGATCTTGACGAAGAACTTAAA
TTGTTACGCGATGAGTTGGAATCAGCTACTGGTCAAAGACTTACTCGTGCAATTAAACGT
TTAGAAGTTGTTGAATCATTCCGTAATTCAGGTAACAAACCTTCATGGATGATTTTAGAT
GTACTTCCAATCATCCCACCAGAAATTCGTCCAATGGTTCAATTAGATGGTGGACGATTT
GCAACAAGTGACTTAAACGACTTATACCGTCGTGTAATTAATCGAAATAATCGTTTGAAA
CGTTTATTAGATTTAGGTGCACCTGGTATCATCGTTCAAAACGAAAAACGTATGTTACAA
GAAGCCGTTGACGCTTTAATTGATAATGGTCGTCGTGGTCGTCCAGTTACTGGCCCAGGT
AACCGTCCATTAAAATCTTTATCTCATATGTTAAAAGGTAAACAAGGTCGTTTCCGTCAA
AACTTACTTGGTAAACGTGTTGACTATTCAGGACGTTCAGTTATTGCAGTAGGTCCAAGC
TTGAAAATGTACCAATGTGGTTTACCAAAAGAAATGGCACTTGAACTATTTAAACCATTC
GTAATGAAAGAATTAGTTCAACGTGAAATTGCAACTAACATTAAAAATGCGAAGAGTAAA
ATCGAACGTATGGATGATGAAGTTTGGGACGTATTGGAAGAAGTAATTAGAGAACATCCT
GTATTACTTAACCGTGCACCAACACTTCATAGACTTGGTATTCAAGCATTTGAACCAACT
TTAGTTGAAGGTCGTGCGATTCGTCTACATCCACTTGTAACAACAGCTTATAACGCTGAC
TTTGACGGTGACCAAATGGCGGTTCACGTTCCTTTATCAAAAGAGGCACAAGCTGAAGCA
AGAATGTTGATGTTAGCAGCACAAAACATCTTGAACCCTAAAGATGGTAAACCTGTAGTT
ACACCATCACAAGATATGGTACTTGGTAACTATTACCTTACTTTAGAAAGAAAAGATGCA
GTAAATACAGGCGCAATCTTTAATAATACAAATGAAGTATTAAAAGCATATGCAAATGGC
TTTGTACATTTACACACTAGAATTGGTGTACATGCAAGTTCGTTCAATAATCCAACATTT
ACTGAAGAACAAAACAAAAAGATTCTTGCTACGTCAGTAGGTAAAATTATATTCAATGAA
ATCATTCCAGATTCATTTGCTTATATTAATGAACCTACGCAAGAAAACTTAGAAAGAAAG
ACACCAAACAGATATTTCATCGATCCTACAACTTTAGGTGAAGGTGGATTAAAAGAATAC
TTTGAAAATGAAGAATTAATTGAACCTTTCAACAAAAAATTCTTAGGTAATATTATTGCA
GAAGTATTCAACAGATTTAGCATCACTGATACATCAATGATGTTAGACCGTATGAAAGAC
TTAGGATTCAAATTCTCATCTAAAGCTGGTATTACAGTAGGTGTTGCTGATATCGTAGTA
TTACCTGATAAGCAACAAATACTTGATGAGCATGAAAAATTAGTCGACAGAATTACAAAA
CAATTCAACCGTGGTTTAATCACTGAAGAAGAAAGATATAATGCAGTTGTTGAAATTTGG
ACAGATGCAAAAGATCAAATTCAAGGTGAATTGATGCAATCACTTGATAAAACTAACCCA
ATCTTCATGATGAGTGATTCAGGTGCCCGTGGTAACGCATCTAACTTTACACAGTTAGCA
GGTATGCGTGGATTGATGGCCGCACCATCTGGTAAGATTATCGAATTACCAATCACATCT
TCATTCCGTGAAGGTTTAACAGTACTTGAATACTTCATCTCAACTCACGGTGCACGTAAA
GGTCTTGCCGATACAGCACTTAAAACAGCTGACTCAGGATATCTTACTCGTCGTCTTGTT
GACGTGGCACAAGATGTTATTGTTCGTGAAGAAGACTGTGGTACTGATAGAGGTTTATTA
GTTTCTGATATTAAAGAAGGTACAGAAATGATTGAACCATTTATCGAACGTATTGAAGGT
CGTTATTCTAAAGAAACAATTCGTCATCCTGAAACTGATGAAATAATCATTCGTCCTGAT
GAATTAATTACACCTGAAATTGCTAAGAAAATTACAGATGCTGGTATTGAACAAATGTAT
ATTCGCTCAGCATTTACTTGTAACGCACGACATGGTGTTTGTGAAAAATGTTACGGTAAA
AACCTTGCTACTGGTGAAAAAGTTGAAGTTGGTGAAGCAGTTGGTACAATTGCAGCCCAA
TCTATCGGTGAACCAGGTACACAGCTTACAATGCGTACATTCCATACAGGTGGGGTAGCA
GGTAGCGATATCACACAAGGTCTTCCTCGTATTCAAGAGATTTTCGAAGCACGTAACCCT
AAAGGTCAAGCGGTAATTACGGAAATCGAAGGTGTCGTAGAAGATATTAAATTAGCAAAA
GATAGACAACAAGAAATTGTTGTTAAAGGTGCTAATGAAACAAGATCATACCTTGCTTCA
GGTACTTCAAGAATTATTGTAGAAATCGGTCAACCAGTTCAACGTGGTGAAGTATTAACT
GAAGGTTCTATTGAACCTAAGAATTACTTATCTGTTGCTGGATTAAACGCGACTGAAAGC
TACTTATTAAAAGAAGTACAAAAAGTTTACCGTATGCAAGGTGTAGAAATCGACGATAAA
CACGTTGAGGTTATGGTTCGACAAATGTTACGTAAAGTTAGAATTATCGAAGCAGGTGAT
ACGAAGTTATTACCAGGTTCATTAGTTGATATTCATAACTTTACAGATGCAAATAGAGAA
GCATTTAAACACCGTAAGCGTCCTGCAACAGCTAAACCAGTATTACTTGGTATTACTAAA
GCATCACTTGAAACAGAAAGTTTCTTATCTGCAGCATCATTCCAAGAAACAACAAGAGTT
CTTACAGATGCAGCAATTAAAGGTAAGCGTGATGACTTATTAGGTCTTAAAGAAAACGTA
ATTATTGGTAAGTTAATTCCAGCTGGTACTGGTATGAGACGTTATAGCGACGTAAAATAC
GAAAAAACAGCTAAACCAGTTGCAGAAGTTGAATCTCAAACTGAAGTAACGGAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS02940 [old locus tag: NWMN_0505 ]
- symbol: NWMN_RS02940
- description: DNA-directed RNA polymerase subunit beta'
- length: 1198
- theoretical pI: 7.01614
- theoretical MW: 134273
- GRAVY: -0.354341
⊟Function[edit | edit source]
- reaction: EC 2.7.7.6? ExPASyDNA-directed RNA polymerase Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
- TIGRFAM: Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase, beta' subunit (TIGR02386; EC 2.7.7.6; HMM-score: 1883.6)and 4 moreDNA-directed RNA polymerase, gamma subunit (TIGR02387; EC 2.7.7.6; HMM-score: 843.5)Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase, beta subunit (TIGR02388; EC 2.7.7.6; HMM-score: 645.3)DNA-directed RNA polymerase subunit A' (TIGR02390; EC 2.7.7.6; HMM-score: 320.8)Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase, subunit A (TIGR02389; EC 2.7.7.6; HMM-score: 115)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: no clan defined RNA_pol_Rpb1_1; RNA polymerase Rpb1, domain 1 (PF04997; HMM-score: 294.8)and 5 moreRNA_pol_Rpb1_5; RNA polymerase Rpb1, domain 5 (PF04998; HMM-score: 233.5)RNA_pol_Rpb1_2; RNA polymerase Rpb1, domain 2 (PF00623; HMM-score: 140.1)RNA_pol_Rpb1_3; RNA polymerase Rpb1, domain 3 (PF04983; HMM-score: 117.5)RNA_pol_Rpb1_4; RNA polymerase Rpb1, domain 4 (PF05000; HMM-score: 54.7)Hybrid (CL0105) Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 17.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.146282
- TAT(Tat/SPI): 0.006504
- LIPO(Sec/SPII): 0.007747
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFGPTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIPSRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAKMGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMILDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSVIAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVLEEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHVPLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFNNTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSFAYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFNRFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQFNRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLAGMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRRLVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEIIIRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGVVEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYLSVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSLVDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAIKGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
NWMN_RS00030 DNA gyrase subunit A [1] (data from MRSA252) NWMN_RS01060 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [1] (data from MRSA252) NWMN_RS02955 30S ribosomal protein S7 [1] (data from MRSA252) NWMN_RS02960 elongation factor G [1] (data from MRSA252) NWMN_RS02965 elongation factor Tu [1] (data from MRSA252) NWMN_RS03640 LysR family transcriptional regulator [1] (data from MRSA252) NWMN_RS06245 isoleucine--tRNA ligase [1] (data from MRSA252) NWMN_RS06490 50S ribosomal protein L19 [1] (data from MRSA252) NWMN_RS07735 hypothetical protein [1] (data from MRSA252) NWMN_RS08090 elongation factor P [1] (data from MRSA252) NWMN_RS08865 aldehyde dehydrogenase [1] (data from MRSA252) NWMN_RS08995 universal stress protein UspA [1] (data from MRSA252) NWMN_RS11175 molecular chaperone GroEL [1] (data from MRSA252) NWMN_RS11795 purine-nucleoside phosphorylase [1] (data from MRSA252) NWMN_RS12350 50S ribosomal protein L6 [1] (data from MRSA252) NWMN_RS12380 30S ribosomal protein S17 [1] (data from MRSA252) NWMN_RS12400 50S ribosomal protein L22 [1] (data from MRSA252) NWMN_RS12705 octopine dehydrogenase [1] (data from MRSA252) NWMN_RS14560 arginine deiminase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)