From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS02960 [old locus tag: NWMN_0509 ]
  • pan locus tag?: SAUPAN002319000
  • symbol: NWMN_RS02960
  • pan gene symbol?: fusA
  • synonym:
  • product: elongation factor G

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS02960 [old locus tag: NWMN_0509 ]
  • symbol: NWMN_RS02960
  • product: elongation factor G
  • replicon: chromosome
  • strand: +
  • coordinates: 584949..587030
  • length: 2082
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (584949..587030) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0509

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCTAGAGAATTTTCATTAGAAAAAACTCGTAATATCGGTATCATGGCTCACATTGAT
    GCTGGTAAAACGACTACGACTGAACGTATTCTTTATTACACTGGCCGTATCCACAAAATT
    GGTGAAACACACGAAGGTGCTTCACAAATGGACTGGATGGAGCAAGAACAAGACCGTGGT
    ATTACTATCACATCTGCTGCAACAACAGCAGCTTGGGAAGGTCACCGTGTAAACATTATC
    GATACACCTGGACACGTAGACTTCACTGTAGAAGTTGAACGTTCATTACGTGTACTTGAC
    GGAGCAGTTACAGTACTTGATGCACAATCAGGTGTTGAACCTCAAACTGAAACAGTTTGG
    CGTCAGGCTACAACTTATGGTGTTCCACGTATCGTATTTGTAAACAAAATGGACAAATTA
    GGTGCTAACTTCGAATACTCTGTAAGTACATTACATGATCGTTTACAAGCTAACGCTGCT
    CCAATCCAATTACCAATTGGTGCGGAAGACGAATTCGAAGCAATCATTGACTTAGTTGAA
    ATGAAATGTTTCAAATATACAAATGATTTAGGTACTGAAATTGAAGAAATTGAAATTCCT
    GAAGACCACTTAGATAGAGCTGAAGAAGCTCGTGCTAGCTTAATCGAAGCAGTTGCAGAA
    ACTAGCGACGAATTAATGGAAAAATATCTTGGTGACGAAGAAATTTCAGTTTCTGAATTA
    AAAGAAGCTATCCGCCAAGCTACTACTAACGTAGAATTCTACCCAGTACTTTGTGGTACA
    GCTTTCAAAAACAAAGGTGTTCAATTAATGCTTGACGCTGTAATTGATTACTTACCTTCA
    CCACTAGACGTTAAACCAATTATTGGTCACCGTGCTAGCAACCCTGAAGAAGAAGTAATC
    GCGAAAGCAGACGATTCAGCTGAATTCGCTGCATTAGCGTTCAAAGTTATGACTGACCCT
    TATGTTGGCAAATTAACATTCTTCCGTGTGTATTCAGGTACAATGACATCTGGTTCATAC
    GTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCT
    AACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTT
    AAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCA
    ATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCAA
    GATAAAATGACTCAAGCTTTAGTTAAATTACAAGAAGAAGACCCAACATTCCATGCACAC
    ACTGACGAAGAAACTGGACAAGTTATCATCGGTGGTATGGGTGAGCTTCACTTAGACATC
    TTAGTAGACCGTATGAAGAAAGAATTCAACGTTGAATGTAACGTAGGTGCTCCAATGGTT
    TCATATCGTGAAACATTCAAATCATCTGCACAAGTTCAAGGTAAATTCTCTCGTCAATCT
    GGTGGTCGTGGTCAATACGGTGATGTTCACATTGAATTCACACCAAACGAAACAGGCGCA
    GGTTTCGAATTCGAAAACGCTATCGTTGGTGGTGTAGTTCCTCGTGAATACATTCCATCA
    GTAGAAGCTGGTCTTAAAGATGCTATGGAAAATGGTGTTTTAGCAGGTTATCCTTTAATT
    GATGTTAAAGCTAAATTATATGATGGTTCATACCATGATGTCGATTCATCTGAAATGGCC
    TTCAAAATTGCTGCATCATTAGCACTTAAAGAAGCTGCTAAAAAATGTGATCCTGTAATC
    TTAGAACCAATGATGAAAGTAACTATTGAAATGCCTGAAGAGTACATGGGTGATATCATG
    GGTGACGTAACATCTCGTCGTGGACGTGTTGATGGTATGGAACCTCGTGGTAATGCACAA
    GTTGTTAATGCTTATGTACCACTTTCAGAAATGCTCGGTTATGCAACATCATTACGTTCA
    CACACTCAAGGTCGCGGTACTTACACTATGTACTTCGATCACTATGCTGAAATTCCAAAA
    TCAATCGCTGAAGATATTATCAAGAAAAATAAAGGTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2082

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS02960 [old locus tag: NWMN_0509 ]
  • symbol: NWMN_RS02960
  • description: elongation factor G
  • length: 693
  • theoretical pI: 4.53897
  • theoretical MW: 76615
  • GRAVY: -0.346032

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Translation factors translation elongation factor G (TIGR00484; HMM-score: 1202.5)
    and 16 more
    Genetic information processing Protein synthesis Translation factors translation elongation factor aEF-2 (TIGR00490; HMM-score: 345.2)
    Cellular processes Cellular processes Adaptations to atypical conditions GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 246.5)
    Genetic information processing Protein synthesis Translation factors GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 246.5)
    Signal transduction Regulatory functions Other GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 246.5)
    Genetic information processing Protein synthesis Translation factors peptide chain release factor 3 (TIGR00503; HMM-score: 206.9)
    Unknown function General elongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 191.2)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 146.9)
    Genetic information processing Protein synthesis Translation factors translation elongation factor Tu (TIGR00485; HMM-score: 59.4)
    Genetic information processing Protein synthesis Translation factors translation elongation factor EF-1, subunit alpha (TIGR00483; HMM-score: 58.2)
    Genetic information processing Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 52.5)
    Genetic information processing Protein synthesis Translation factors selenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 39.2)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfate adenylyltransferase, large subunit (TIGR02034; EC 2.7.7.4; HMM-score: 36.6)
    Genetic information processing Protein synthesis Translation factors translation initiation factor aIF-2 (TIGR00491; HMM-score: 27.5)
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 26.6)
    translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 26.2)
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 11.3)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    P-loop_NTPase (CL0023) GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 234.5)
    and 7 more
    S5 (CL0329) EFG_IV; Elongation factor G, domain IV (PF03764; HMM-score: 152.7)
    EF-G_C (CL0437) EFG_II; Elongation Factor G, domain II (PF14492; HMM-score: 113.9)
    EFG_C; Elongation factor G C-terminus (PF00679; HMM-score: 108.3)
    EFTPs (CL0575) GTP_EFTU_D2; Elongation factor Tu domain 2 (PF03144; HMM-score: 54.8)
    EF-G_C (CL0437) RF3_C; Class II release factor RF3, C-terminal domain (PF16658; HMM-score: 35.4)
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 21)
    AIG1; AIG1 family (PF04548; HMM-score: 17.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00882
    • TAT(Tat/SPI): 0.001526
    • LIPO(Sec/SPII): 0.001261
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAAPIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAVAETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVIDYLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGTMTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGEKNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVIIGGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKLYDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVTSRRGRVDGMEPRGNAQVVNAYVPLSEMLGYATSLRSHTQGRGTYTMYFDHYAEIPKSIAEDIIKKNKGE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_RS02965elongation factor Tu  [1] (data from MRSA252)
    NWMN_RS03640LysR family transcriptional regulator  [1] (data from MRSA252)
    NWMN_RS0657530S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_RS08930pyruvate kinase  [1] (data from MRSA252)
    NWMN_RS08995universal stress protein UspA  [1] (data from MRSA252)
    NWMN_RS1234030S ribosomal protein S5  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_RS02960&oldid=77709"