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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1651 [new locus tag: NWMN_RS09265 ]
  • pan locus tag?: SAUPAN004441000
  • symbol: leuS
  • pan gene symbol?: leuS
  • synonym:
  • product: leucyl-tRNA synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1651 [new locus tag: NWMN_RS09265 ]
  • symbol: leuS
  • product: leucyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1845657..1848074
  • length: 2418
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    GTGTTGAATTACAACCACAATCAAATTGAAAAGAAATGGCAAGACTATTGGGACGAAAAT
    AAAACATTTAAAACAAATGATAACTTAGGTCAAAAGAAATTTTATGCTTTAGACATGTTT
    CCATATCCATCAGGTGCTGGTTTACATGTTGGACATCCTGAGGGCTATACAGCAACAGAT
    ATCATTTCAAGATATAAAAGAATGCAAGGATATAATGTATTACATCCGATGGGGTGGGAT
    GCATTCGGATTACCAGCAGAGCAATATGCTTTAGACACTGGCAACGACCCACGTGAATTT
    ACAAAGAAAAATATCCAAACTTTTAAACGACAAATTAAAGAATTAGGGTTCAGTTATGAT
    TGGGATCGTGAAGTTAATACAACAGATCCAGAATACTATAAATGGACACAGTGGATTTTC
    ATACAGTTATATAACAAAGGTTTAGCATACGTTGATGAAGTTGCAGTTAACTGGTGTCCA
    GCATTAGGCACTGTTTTATCTAACGAAGAAGTGATTGATGGTGTCTCTGAACGTGGTGGA
    CATCCAGTTTATCGTAAGCCGATGAAACAATGGGTACTTAAAATCACAGAATATGCAGAT
    CAATTATTAGCAGATTTAGATGATTTAGATTGGCCTGAGTCTTTAAAAGATATGCAGCGC
    AATTGGATTGGACGTTCTGAAGGGGCCAAAGTTTCATTTGATGTAGATAATACGGAAGGA
    AAAGTAGAAGTATTTACGACTAGACCAGATACAATCTATGGTGCATCATTCTTAGTCTTA
    AGTCCTGAACATGCATTAGTTAATTCAATTACAACAGATGAATATAAAGAAAAAGTAAAA
    GCTTATCAAACAGAAGCTTCTAAAAAGTCAGATTTAGAACGTACAGATTTAGCAAAAGAT
    AAATCAGGTGTATTTACTGGTGCATATGCAACTAATCCTTTATCTGGTGAAAAAGTACAA
    ATTTGGATTGCTGATTATGTATTATCAACATATGGTACTGGAGCAATTATGGCAGTACCA
    GCGCATGATGACAGAGATTATGAATTTGCTAAAAAGTTTGATTTGCCAATCATTGAAGTC
    ATCGAAGGTGGAAATGTTGAAGAAGCAGCATACACTGGTGAAGGTAAACATATTAATTCT
    GGTGAACTTGATGGTTTAGAAAATGAAGCGGCAATTACTAAAGCTATTCAATTATTAGAG
    CAAAAAGGTGCTGGCGAAAAGAAAGTTAATTACAAATTAAGAGATTGGTTATTCAGTCGT
    CAGCGTTATTGGGGCGAACCAATTCCTGTCATTCATTGGGAAGATGGAACAATGACAACT
    GTTCCTGAAGAAGAGCTACCATTGTTGTTACCTGAAACAGATGAAATCAAGCCATCAGGG
    ACTGGTGAGTCTCCACTAGCTAATATTGATTCATTTGTAAATGTTGTAGATGAAAAAACA
    GGTATGAAAGGACGTCGTGAAACAAATACAATGCCACAATGGGCAGGTAGTTGTTGGTAT
    TATTTACGTTACATCGATCCTAAAAATGAAAATATGTTAGCAGATCCTGAAAAATTAAAA
    CATTGGTTACCTGTTGATTTATATATCGGTGGAGTAGAACATGCGGTTCTTCACTTATTA
    TATGCAAGATTTTGGCATAAAGTCCTTTATGATTTGGCTATCGTACCTACTAAAGAACCT
    TTCCAAAAATTATTTAACCAAGGTATGATTTTAGGAGAAGGTAATGAGAAGATGAGTAAA
    TCTAAAGGAAATGTAATCAATCCTGATGATATAGTACAGTCTCATGGTGCAGATACTTTG
    CGTCTTTACGAAATGTTTATGGGACCTTTAGATGCTGCAATTGCATGGAGTGAAAAAGGA
    TTAGATGGGTCTCGTCGATTCTTAGATCGCGTATGGCGTTTAATGGTAAATGAAGATGGG
    ACATTGAGTTCAAAAATTGTAACTACAAATAATAAATCTTTAGATAAAGTTTATAACCAA
    ACTGTTAAAAAGGTAACAGAAGACTTTGAAACATTAGGATTTAATACTGCTATTAGTCAA
    TTAATGGTATTTATTAATGAGTGTTATAAAGTTGATGAAGTTTATAAACCTTACATTGAA
    GGCTTCGTTAAAATGTTAGCACCTATTGCACCACATATCGGTGAAGAATTATGGTCAAAA
    TTAGGACATGAAGAGTCTATTACGTACCAACCTTGGCCAACTTATGACGAAGCACTACTT
    GTAGATGATGAAGTAGAAATCGTTGTTCAAGTGAATGGTAAATTGAGAGCTAAAATTAAA
    ATTGCTAAAGATACATCAAAAGAAGAAATGCAAGAAATTGCCTTATCTAATGACAATGTT
    AAAGCGAGTATTGAAGGTAAAGACATCATGAAAGTCATCGCTGTTCCTCAAAAATTAGTC
    AATATTGTAGCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2418

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1651 [new locus tag: NWMN_RS09265 ]
  • symbol: LeuS
  • description: leucyl-tRNA synthetase
  • length: 805
  • theoretical pI: 4.78881
  • theoretical MW: 91785.3
  • GRAVY: -0.498509

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.4?  ExPASy
    Leucine--tRNA ligase ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 1186.6)
    and 6 more
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 310.1)
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 228.8)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 227.8)
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 114.3)
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 37.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 26.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    HUP (CL0039) tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 245.4)
    tRNA-synt_1_2; Leucyl-tRNA synthetase, Domain 2 (PF13603; HMM-score: 227)
    and 5 more
    tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 96.9)
    DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA (PF08264; HMM-score: 82.7)
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 25.9)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 18.3)
    tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015459
    • TAT(Tat/SPI): 0.002462
    • LIPO(Sec/SPII): 0.001287
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCPALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQKLVNIVAK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_1605(ackA)acetate kinase  [1] (data from MRSA252)
    NWMN_0372(ahpC)alkyl hydroperoxide reductase subunit C  [1] (data from MRSA252)
    NWMN_2534(arcA)arginine deiminase  [1] (data from MRSA252)
    NWMN_2533(arcB)ornithine carbamoyltransferase  [1] (data from MRSA252)
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_2042(deoD)purine nucleoside phosphorylase  [1] (data from MRSA252)
    NWMN_1483(dnaK)molecular chaperone DnaK  [1] (data from MRSA252)
    NWMN_0745(eno)phosphopyruvate hydratase  [1] (data from MRSA252)
    NWMN_1141(fabG)3-oxoacyl-[acyl-carrier protein] reductase  [1] (data from MRSA252)
    NWMN_1625(fhs)formate--tetrahydrofolate ligase  [1] (data from MRSA252)
    NWMN_1096(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_0741(gapA)glyceraldehyde 3-phosphate dehydrogenase 1  [1] (data from MRSA252)
    NWMN_2056(glmS)glucosamine--fructose-6-phosphate aminotransferase  [1] (data from MRSA252)
    NWMN_1468(glyS)glycyl-tRNA synthetase  [1] (data from MRSA252)
    NWMN_1417(gnd)6-phosphogluconate dehydrogenase  [1] (data from MRSA252)
    NWMN_0380(guaB)inosine-5'-monophosphate dehydrogenase  [1] (data from MRSA252)
    NWMN_0828(gudB)NAD-specific glutamate dehydrogenase  [1] (data from MRSA252)
    NWMN_0176(ldh1)L-lactate dehydrogenase  [1] (data from MRSA252)
    NWMN_1826(map1)methionine aminopeptidase  [1] (data from MRSA252)
    NWMN_0700(nrdE)ribonucleotide-diphosphate reductase subunit alpha  [1] (data from MRSA252)
    NWMN_0701(nrdF)ribonucleotide-diphosphate reductase subunit beta  [1] (data from MRSA252)
    NWMN_2040(pdp)pyrimidine-nucleoside phosphorylase  [1] (data from MRSA252)
    NWMN_0162(pflB)formate acetyltransferase  [1] (data from MRSA252)
    NWMN_0833(pgi)glucose-6-phosphate isomerase  [1] (data from MRSA252)
    NWMN_0742(pgk)phosphoglycerate kinase  [1] (data from MRSA252)
    NWMN_0744(pgm)phosphoglyceromutase  [1] (data from MRSA252)
    NWMN_1183(pnpA)polynucleotide phosphorylase/polyadenylase  [1] (data from MRSA252)
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_2137(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    NWMN_0014(rplI)50S ribosomal protein L9  [1] (data from MRSA252)
    NWMN_0501(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_0502(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    NWMN_2125(rplQ)50S ribosomal protein L17  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_1549(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    NWMN_2150(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    NWMN_0504(rpoB)DNA-directed RNA polymerase subunit beta  [1] (data from MRSA252)
    NWMN_1166(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_2146(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_2119(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    NWMN_2148(rpsS)30S ribosomal protein S19  [1] (data from MRSA252)
    NWMN_1569(tig)trigger factor  [1] (data from MRSA252)
    NWMN_1167(tsf)elongation factor Ts  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_0632hypothetical protein  [1] (data from MRSA252)
    NWMN_0655MarR family regulatory protein  [1] (data from MRSA252)
    NWMN_0776glycine cleavage system protein H  [1] (data from MRSA252)
    NWMN_0811hypothetical protein  [1] (data from MRSA252)
    NWMN_0949phosphocarrier protein HPr  [1] (data from MRSA252)
    NWMN_1672putative translaldolase  [1] (data from MRSA252)
    NWMN_1831ferritin  [1] (data from MRSA252)
    NWMN_2086alkaline shock protein 23  [1] (data from MRSA252)
    NWMN_2503fructose-1,6-bisphosphate aldolase  [1] (data from MRSA252)
    NWMN_2504malate:quinone oxidoreductase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: T-box(Leu) (transcription antitermination) regulon
    T-box(Leu)(RNA)important in Amino acid metabolism; regulatory site identified based on RegPrecise data for N315 RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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