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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus Newman
locus tag: NWMN_0701 [new locus tag: NWMN_RS03965 ]
pan locus tag^?: SAUPAN002631000
symbol: nrdF
pan gene symbol^?: nrdF
synonym:
product: ribonucleotide-diphosphate reductase subunit beta

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: NWMN_0701 [new locus tag: NWMN_RS03965 ]
symbol: nrdF
product: ribonucleotide-diphosphate reductase subunit beta
replicon: chromosome
strand: +
coordinates: 786847..787818
length: 972
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID: 5332208 NCBI
RefSeq: YP_001331735 NCBI
BioCyc:
MicrobesOnline: 3706248 MicrobesOnline

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

1
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961
ATGATAGCTGTTAATTGGAACACACAAGAAGATATGACGAATATGTTTTGGAGACAAAAT
ATATCTCAAATGTGGGTTGAAACAGAATTTAAAGTATCAAAAGACATTGCAAGTTGGAAG
ACTTTATCTGAAGCTGAACAAGACACATTTAAAAAAGCATTAGCTGGTTTAACAGGCTTA
GATACACATCAAGCAGATGATGGCATGCCTTTAGTTATGCTACATACGACTGACTTAAGG
AAAAAAGCAGTTTATTCATTTATGGCGATGATGGAGCAAATACACGCGAAAAGCTATTCA
CATATTTTCACAACACTATTACCATCTAGTGAAACAAACTACCTATTAGATGAATGGGTT
TTAGAGGAACCCCATTTAAAATATAAATCTGATAAAATTGTTGCTAATTATCACAAACTT
TGGGGTAAAGAAGCTTCGATATACGACCAATATATGGCCAGAGTTACGAGTGTATTTTTA
GAAACATTCTTATTCTTCTCAGGTTTCTATTATCCACTATATCTTGCTGGTCAAGGGAAA
ATGACGACATCAGGTGAAATCATTCGTAAAATTCTTTTAGATGAATCTATTCATGGTGTA
TTTACCGGTTTAGATGCACAGCATTTACGAAATGAACTATCTGAAAGTGAGAAACAAAAA
GCAGATCAAGAAATGTATAAATTGCTAAATGACTTGTATTTAAATGAAGAGTCATACACA
AAAATGTTATACGATGATCTTGGAATCACTGAAGATGTGCTAAACTATGTTAAATATAAT
GGAAACAAAGCACTTTCAAACTTAGGCTTTGAACCTTATTTTGAGGAACGTGAATTTAAC
CCAATCATTGAGAATGCCTTAGATACAACAACTAAAAACCATGACTTCTTCTCAGTAAAA
GGTGATGGTTATGTATTAGCATTAAACGTAGAAGCATTACAAGATGATGACTTTGTATTT
GACAACAAATAA
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972

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: NWMN_0701 [new locus tag: NWMN_RS03965 ]
symbol: NrdF
description: ribonucleotide-diphosphate reductase subunit beta
length: 323
theoretical pI: 4.52439
theoretical MW: 37513.1
GRAVY: -0.409907

⊟Function[edit | edit source]

reaction:
EC 1.17.4.1? ExPASy
Ribonucleoside-diphosphate reductase 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H₂O = ribonucleoside diphosphate + thioredoxin
TIGRFAM:
Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism ribonucleoside-diphosphate reductase, class 1b, beta subunit (TIGR04171; EC 1.17.4.1; HMM-score: 411.6)
TheSEED :
- Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1)
Nucleosides and Nucleotides Nucleosides and Nucleotides - no subcategory Ribonucleotide reduction Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1)
PFAM:
Ferritin (CL0044) Ribonuc_red_sm; Ribonucleotide reductase, small chain (PF00268; HMM-score: 232.3)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helix: 1
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.6989
- Cytoplasmic Membrane Score: 0.005
- Cell wall & surface Score: 0.0004
- Extracellular Score: 0.2957
LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 0.83
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003485
- TAT(Tat/SPI): 0.000504
- LIPO(Sec/SPII): 0.000367
predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

GI: 151220913 NCBI
RefSeq: YP_001331735 NCBI
UniProt: A0A0H3K6R4 UniProt

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSEAEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHIFTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFLETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEKQKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEEREFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: nrdI > nrdE > nrdF

⊟Regulation[edit | edit source]

regulator: NrdR (repression) regulon
NrdR (TF) important in Deoxyribonucleotide biosynthesis; RegPrecise transcription unit transferred from N315 data RegPrecise

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: data available for COL

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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