PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1379 [new locus tag: NWMN_RS07765 ]
• pan locus tag^?: SAUPAN003930000
• symbol: hepT
• pan gene symbol^?: hepT
• synonym:
• product: heptaprenyl diphosphate synthase component II

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1379 [new locus tag: NWMN_RS07765 ]
• symbol: hepT
• product: heptaprenyl diphosphate synthase component II
• replicon: chromosome
• strand: -
• coordinates: 1545756..1546724
• length: 969
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332201 NCBI
• RefSeq: YP_001332413 NCBI
• BioCyc:
• MicrobesOnline: 3706931 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  GTGATTAACGTGGCAAAGTTAAACATGAACAATGAAATTAAGAAAGTGGAACAACGACTT
  GAAAAGGCAATAAAAAGTAAAGATTCTGTATTAGAACAGGCATCATTACACTTATTGTCT
  TCTGGTGGTAAAAGAGTACGCCCAGCATTTGTTATTCTGAGTAGCCAATTTGGCAAAGAT
  GAACAAACGTCTGAACAAACGTATCAAGTTGCAGTCGCATTAGAGTTAATTCATATGGCA
  ACACTTGTTCATGATGACGTTATTGATAAAAGCGACAAGCGTCGAGGCAAGTTAACCATA
  TCAAAGAAATGGGATCAGACAACTGCTATTTTAACTGGGAATTTTTTATTGGCATTAGGA
  CTTGAACACTTAATGGCCGTTAAAGATAATCGTGTACATCAATTGATATCTGAATCTATC
  GTTGATGTTTGTAGAGGGGAACTTTTCCAATTTCAAGACCAATTTAACAGTCAACAGACA
  ATTATTAATTATTTACGACGTATCAATCGCAAAACAGCACTGTTAATTCAAATATCAACT
  GAAGTTGGTGCAATTACTTCTCAATCTGATAAAGAGACTGTACGAAAATTGAAAATGATT
  GGTCATTATATAGGTATGAGCTTCCAAATCATTGATGATGTATTAGACTTCACAAGTACC
  GAAAAGAAATTAGGTAAGCCGGTCGGAAGTGATTTGCTTAATGGTCATATTACGTTACCG
  ATTTTATTAGAAATGCGTAAAAATCCAGACTTCAAATTGAAAATCGAACAGTTACGTCGT
  GATAGTGAACGCAAAGAATTTGAAGAATGTATCCAAATCATTAGAAAATCTGACAGCATC
  GATGAGGCTAAGGCAGTAAGTTCGAAGTATTTAAGTAAAGCTTTGAATTTGATTTCTGAG
  TTACCAGATGGACATCCGAAATCACTACTTTTAAGTTTGACGAAAAAAATGGGTTCAAGA
  AACACGTAG
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⊟Protein

⊟General

• locus tag: NWMN_1379 [new locus tag: NWMN_RS07765 ]
• symbol: HepT
• description: heptaprenyl diphosphate synthase component II
• length: 322
• theoretical pI: 9.89584
• theoretical MW: 36469
• GRAVY: -0.331988

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone heptaprenyl diphosphate synthase component II (TIGR02748; EC 2.5.1.30; HMM-score: 378.9)
  and 1 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone solanesyl diphosphate synthase (TIGR02749; EC 2.5.1.-; HMM-score: 242.6)
• TheSEED  :
  • Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid scratch  Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
  and 3 more
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoinds for Quinones  Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Polyprenyl Diphosphate Biosynthesis  Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
  Regulation and Cell signaling Regulation and Cell signaling - no subcategory Sex pheromones in Enterococcus faecalis and other Firmicutes  Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
• PFAM:
  Terp_synthase (CL0613) polyprenyl_synt; Polyprenyl synthetase (PF00348; HMM-score: 238.2)
  and 4 more
  HEPPP_synt_1; Heptaprenyl diphosphate synthase (HEPPP synthase) subunit 1 (PF07307; HMM-score: 28.5)
  bHLH (CL0827) bHLH_INO2; INO2 bHLH domain (PF23179; HMM-score: 17.7)
  no clan defined Baculo_p33; Baculovirus P33 (PF05214; HMM-score: 16.5)
  NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 14.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8848
  • Cytoplasmic Membrane Score: 0.105
  • Cell wall & surface Score: 0.0012
  • Extracellular Score: 0.009
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00786
  • TAT(Tat/SPI): 0.001301
  • LIPO(Sec/SPII): 0.000528
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221591 NCBI
• RefSeq: YP_001332413 NCBI
• UniProt: A0A0H3K8L1 UniProt

⊟Protein sequence

• MINVAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVRPAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWDQTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIINYLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTEKKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDSIDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ndk < hepT < ubiE < NWMN_1381

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications