PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1359 [new locus tag: SAUSA300_RS07410 ]
• pan locus tag^?: SAUPAN003930000
• symbol: SAUSA300_1359
• pan gene symbol^?: hepT
• synonym:
• product: polyprenyl synthetase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1359 [new locus tag: SAUSA300_RS07410 ]
• symbol: SAUSA300_1359
• product: polyprenyl synthetase
• replicon: chromosome
• strand: -
• coordinates: 1526290..1527234
• length: 945
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913985 NCBI
• RefSeq: YP_494056 NCBI
• BioCyc: see SAUSA300_RS07410
• MicrobesOnline: 1292874 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  901
  
  ATGAACAATGAAATTAAGAAAGTGGAACAACGACTTGAAAAGGCAATAAAAAGTAAAGAT
  TCTGTATTAGAACAGGCATCATTACACTTATTGTCTTCTGGTGGTAAAAGAGTACGCCCA
  GCATTTGTTATTCTGAGTAGCCAATTTGGCAAAGATGAACAAACGTCTGAACAAACGTAT
  CAAGTTGCAGTCGCATTAGAGTTAATTCATATGGCAACACTTGTTCATGATGACGTTATT
  GATAAAAGCGACAAGCGTCGAGGCAAGTTAACCATATCAAAGAAATGGGATCAGACAACT
  GCTATTTTAACTGGGAATTTTTTATTGGCATTAGGACTTGAACACTTAATGGCCGTTAAA
  GATAATCGTGTACATCAATTGATATCTGAATCTATCGTTGATGTTTGTAGAGGGGAACTT
  TTCCAATTTCAAGACCAATTTAACAGTCAACAGACAATTATTAATTATTTACGACGTATC
  AATCGCAAAACAGCACTGTTAATTCAAATATCAACTGAAGTTGGTGCAATTACTTCTCAA
  TCTGATAAAGAGACTGTACGAAAATTGAAAATGATTGGTCATTATATAGGTATGAGCTTC
  CAAATCATTGATGATGTATTAGACTTCACAAGTACCGAAAAGAAATTAGGTAAGCCGGTC
  GGAAGTGATTTGCTTAATGGTCATATTACGTTACCGATTTTATTAGAAATGCGTAAAAAT
  CCAGACTTCAAATTGAAAATCGAACAGTTACGTCGTGATAGTGAACGCAAAGAATTTGAA
  GAATGTATCCAAATCATTAGAAAATCTGACAGCATCGATGAGGCTAAGGCAGTAAGTTCG
  AAGTATTTAAGTAAAGCTTTGAATTTGATTTCTGAGTTACCAGATGGACATCCGAAATCA
  CTACTTTTAAGTTTGACGAAAAAAATGGGTTCAAGAAACACGTAG
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  945
  

⊟Protein

• 5H9D
  PDB

Protein Data Bank: 5H9D

⊟General

• locus tag: SAUSA300_1359 [new locus tag: SAUSA300_RS07410 ]
• symbol: SAUSA300_1359
• description: polyprenyl synthetase
• length: 314
• theoretical pI: 9.79656
• theoretical MW: 35584.9
• GRAVY: -0.357325

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone heptaprenyl diphosphate synthase component II (TIGR02748; EC 2.5.1.30; HMM-score: 377.5)
  and 1 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone solanesyl diphosphate synthase (TIGR02749; EC 2.5.1.-; HMM-score: 242.2)
• TheSEED  :
  • Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid scratch  Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
  and 3 more
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoinds for Quinones  Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Polyprenyl Diphosphate Biosynthesis  Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
  Regulation and Cell signaling Regulation and Cell signaling - no subcategory Sex pheromones in Enterococcus faecalis and other Firmicutes  Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
• PFAM:
  Terp_synthase (CL0613) polyprenyl_synt; Polyprenyl synthetase (PF00348; HMM-score: 222.8)
  and 3 more
  HEPPP_synt_1; Heptaprenyl diphosphate synthase (HEPPP synthase) subunit 1 (PF07307; HMM-score: 29.4)
  no clan defined Baculo_p33; Baculovirus P33 (PF05214; HMM-score: 16.5)
  NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 15.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007133
  • TAT(Tat/SPI): 0.001885
  • LIPO(Sec/SPII): 0.001011
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160661 NCBI
• RefSeq: YP_494056 NCBI
• UniProt: A0A0H2XFM2 UniProt

⊟Protein sequence

• MNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVRPAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWDQTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIINYLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTEKKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDSIDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1359 < ubiE

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications