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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01486
  • pan locus tag?: SAUPAN003930000
  • symbol: SAOUHSC_01486
  • pan gene symbol?: hepT
  • synonym:
  • product: heptaprenyl diphosphate syntase component II

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01486
  • symbol: SAOUHSC_01486
  • product: heptaprenyl diphosphate syntase component II
  • replicon: chromosome
  • strand: -
  • coordinates: 1442912..1443856
  • length: 945
  • essential: no [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAACAATGAAATTAAGAAAGTGGAACAACGACTTGAAAAGGCAATAAAAAGTAAAGAT
    TCTGTATTAGAACAGGCATCATTACACTTATTGTCTTCTGGTGGTAAAAGAGTACGCCCA
    GCATTTGTTATTCTGAGTAGCCAATTTGGCAAAGATGAACAAACGTCTGAACAAACGTAT
    CAAGTTGCAGTCGCATTAGAGTTAATTCATATGGCAACACTTGTTCATGATGACGTTATT
    GATAAAAGCGACAAGCGTCGAGGCAAGTTAACCATATCAAAGAAATGGGATCAGACAACT
    GCTATTTTAACTGGGAATTTTTTATTGGCATTAGGACTTGAACACTTAATGGCCGTTAAA
    GATAATCGTGTACATCAATTGATATCTGAATCTATCGTTGATGTTTGTAGAGGGGAACTT
    TTCCAATTTCAAGACCAATTTAACAGTCAACAGACAATTATTAATTATTTACGACGTATC
    AATCGCAAAACAGCACTGTTAATTCAAATATCAACTGAAGTTGGTGCAATTACTTCTCAA
    TCTGATAAAGAGACTGTACGAAAATTGAAAATGATTGGTCATTATATAGGTATGAGCTTC
    CAAATCATTGATGATGTATTAGACTTCACAAGTACCGAAAAGAAATTAGGTAAGCCGGTC
    GGAAGTGATTTGCTTAATGGTCATATTACGTTACCGATTTTATTAGAAATGCGTAAAAAT
    CCAGACTTCAAATTGAAAATCGAACAGTTACGTCGTGATAGTGAACGCAAAGAATTTGAA
    GAATGTATCCAAATCATTAGAAAATCTGACAGCATCGATGAGGCTAAGGCAGTAAGTTCG
    AAGTATTTAAGTAAAGCTTTGAATTTGATTTCTGAGTTACCAGATGGACATCCGAAATCA
    CTACTTTTAAGTTTGACGAAAAAAATGGGTTCAAGAAACACGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    945

⊟Protein[edit | edit source]

Protein Data Bank: 5H9D

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01486
  • symbol: SAOUHSC_01486
  • description: heptaprenyl diphosphate syntase component II
  • length: 314
  • theoretical pI: 9.79656
  • theoretical MW: 35584.9
  • GRAVY: -0.357325

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.30?  ExPASy
    Heptaprenyl diphosphate synthase (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate = 4 diphosphate + all-trans-heptaprenyl diphosphate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone heptaprenyl diphosphate synthase component II (TIGR02748; EC 2.5.1.30; HMM-score: 377.5)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone solanesyl diphosphate synthase (TIGR02749; EC 2.5.1.-; HMM-score: 242.2)
  • TheSEED  :
    • Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid scratch  Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
    and 3 more
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoinds for Quinones  Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Polyprenyl Diphosphate Biosynthesis  Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Sex pheromones in Enterococcus faecalis and other Firmicutes  Heptaprenyl diphosphate synthase component II (EC 2.5.1.30)
  • PFAM:
    Terp_synthase (CL0613) polyprenyl_synt; Polyprenyl synthetase (PF00348; HMM-score: 238.3)
    and 4 more
    HEPPP_synt_1; Heptaprenyl diphosphate synthase (HEPPP synthase) subunit 1 (PF07307; HMM-score: 28.4)
    bHLH (CL0827) bHLH_INO2; INO2 bHLH domain (PF23179; HMM-score: 18)
    no clan defined Baculo_p33; Baculovirus P33 (PF05214; HMM-score: 16.5)
    NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 14)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8531
    • Cytoplasmic Membrane Score: 0.1364
    • Cell wall & surface Score: 0.0015
    • Extracellular Score: 0.0091
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007133
    • TAT(Tat/SPI): 0.001885
    • LIPO(Sec/SPII): 0.001011
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVRPAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWDQTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIINYLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTEKKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDSIDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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