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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_1374 [new locus tag: NWMN_RS07735 ]
- pan locus tag?: SAUPAN003923000
- symbol: NWMN_1374
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_1374 [new locus tag: NWMN_RS07735 ]
- symbol: NWMN_1374
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 1539606..1540850
- length: 1245
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5330818 NCBI
- RefSeq: YP_001332408 NCBI
- BioCyc:
- MicrobesOnline: 3706926 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201ATGGAAGATATCTATAAATTAATAGACGATATCAATCTACAAAAACTAGAAAATTTAGAC
TCTCGTGTTAATGAAGCAATAACTACTGACAACGATGACGCATTATTTATTCTAGGAGAG
ACACTTTACAATTTTGGATTAATGCCACAAGGTTTGGAAGTATTCCGCGTGTTATATCAC
AAATATCCAGACGAAAGTGAATTGCTGATTTATTTTATTGAAGGTTTAATGTCTGAAAAT
CAAACTGACGAAGCGTTAGAATATTTAAGTTATGTTGAACCATCACCTGAAAAGTTGATG
TTAGAAGCAGATTTATATCAACAAATTAATATGATGGAAGTTGCTATTGATAAATTACAA
GAAGCACTTGAACTAGAGCCAAATGATCCAATAATCCATTTTGCATTGGCTGAAATGTTA
TATTATGATGGTCAATATTTACGTGCTACCTCTGAATACGAAACCGTTTTAGAAACTGGT
GAATATCAAGTTAATGGTGTAAACTTATTCTCTCGTATGGCAGATTGTAGTTTACAAAGT
GGTAACTATAGTGATGCGATTCGCTTATACGATGAAATTAATGAAGATGAAATGACTTCA
GAAGATTATCTCAAAAAAGCCATTTCTTACGATAAAAATGACATCACTCAAGAAGCAATT
AAAATAATGACTACATTACTTTCTAAAGATCCTGATTATATTCAAGGCTACTTGTATTTA
CAATCATTATATGAAAATGAAAAAAATTATCCAGATGCCATTGAAACGGGTAAAGAAGGA
TTACGACTAAGTCAATTTTATAAAGAGTTAATGTATACAACTGGATGTTTGGAAATAGAA
CACGGCGATGCTAATGAAGGTGTATTATTGTTAAAACAAGCATTAGAGGTTGATAATGCT
TACCAAGAACCTTTATTGATTTTAAGCGATTTATATCGTAATGAAGAAGATTATGAAGCA
ATCATTGAATTATTAACATATGTAGATGAAGAAGATTTGGATCCTACATTCATGTGGCAT
TTAGCGTTCGCCTATGGTCAAGAAGAGCGAGATAAAGAAGCTCAACATTTCTTTGAATTA
GCATATCCAACGATGAAAACAAATATTGATTTTATGAGTGATTATTATTTCTATTTAATT
GAAATTGGTCAAAAGGAACAAGCAATTACTATTTTAAATCAATTGTTGGAACTAGAGCCA
AGTAACGAAAATTGGCATGACGAATTATCAAGATTGCAATCTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_1374 [new locus tag: NWMN_RS07735 ]
- symbol: NWMN_1374
- description: hypothetical protein
- length: 414
- theoretical pI: 3.78508
- theoretical MW: 48422.7
- GRAVY: -0.439855
⊟Function[edit | edit source]
- TIGRFAM: putative PEP-CTERM system TPR-repeat lipoprotein (TIGR02917; HMM-score: 73)and 8 moretype IV pilus biogenesis/stability protein PilW (TIGR02521; HMM-score: 45.4)type III secretion low calcium response chaperone LcrH/SycD (TIGR02552; HMM-score: 34.6)tol-pal system protein YbgF (TIGR02795; HMM-score: 30.2)poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA (TIGR03939; HMM-score: 21.1)Transport and binding proteins Amino acids, peptides and amines mitochondrial precursor proteins import receptor (TIGR00990; HMM-score: 20.5)Unknown function General heme biosynthesis-associated TPR protein (TIGR00540; HMM-score: 19)Protein fate Protein and peptide secretion and trafficking outer membrane assembly lipoprotein YfiO (TIGR03302; HMM-score: 17.3)pentatricopeptide repeat domain (TIGR00756; HMM-score: 13.2)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: TPR (CL0020) TPR_19; Tetratricopeptide repeat (PF14559; HMM-score: 79.2)TPR_2; Tetratricopeptide repeat (PF07719; HMM-score: 63.7)and 18 moreTPR_16; Tetratricopeptide repeat (PF13432; HMM-score: 49.3)TPR_6; Tetratricopeptide repeat (PF13174; HMM-score: 43)TPR_14; Tetratricopeptide repeat (PF13428; HMM-score: 40.7)TPR_7; Tetratricopeptide repeat (PF13176; HMM-score: 35)TPR_11; TPR repeat (PF13414; HMM-score: 33)TPR_15; Tetratricopeptide repeat (PF13429; HMM-score: 30.7)ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3 (PF12895; HMM-score: 29.5)TPR_12; Tetratricopeptide repeat (PF13424; HMM-score: 27.7)TPR_8; Tetratricopeptide repeat (PF13181; HMM-score: 25.6)BTAD; Bacterial transcriptional activator domain (PF03704; HMM-score: 23.4)TPR_20; Tetratricopeptide repeat (PF14561; HMM-score: 21.7)TPR_17; Tetratricopeptide repeat (PF13431; HMM-score: 20.5)TPR_9; Tetratricopeptide repeat (PF13371; HMM-score: 20.3)TPR_1; Tetratricopeptide repeat (PF00515; HMM-score: 17.8)SGNH_hydrolase (CL0264) DUF4886; Domain of unknown function (DUF4886) (PF16227; HMM-score: 15.9)TPR (CL0020) SNAP; Soluble NSF attachment protein, SNAP (PF14938; HMM-score: 15)no clan defined MIT; MIT (microtubule interacting and transport) domain (PF04212; HMM-score: 14.8)TPR (CL0020) PPR; PPR repeat (PF01535; HMM-score: 13.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001198
- TAT(Tat/SPI): 0.00011
- LIPO(Sec/SPII): 0.000243
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYNFGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLEADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETGEYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQEAIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGCLEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLDPTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITILNQLLELEPSNENWHDELSRLQS
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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