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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1503 [new locus tag: SACOL_RS07655 ]
  • pan locus tag?: SAUPAN003923000
  • symbol: SACOL1503
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1503 [new locus tag: SACOL_RS07655 ]
  • symbol: SACOL1503
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1543426..1544670
  • length: 1245
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGAAGATATCTATAAATTAATAGACGATATCAATCTACAAAAACTAGAAAATTTAGAC
    TCTCGTGTTAATGAAGCAATAACTACTGACAACGATGACGCATTATTTATTCTAGGAGAG
    ACACTTTACAATTTTGGATTAATGCCACAAGGTTTGGAAGTATTCCGCGTGTTATATCAC
    AAATATCCAGACGAAAGTGAATTGCTGATTTATTTTATTGAAGGTTTAATGTCTGAAAAT
    CAAACTGACGAAGCGTTAGAATATTTAAGTTATGTTGAACCATCACCTGAAAAGTTGATG
    TTAGAAGCAGATTTATATCAACAAATTAATATGATGGAAGTTGCTATTGATAAATTACAA
    GAAGCACTTGAACTAGAGCCAAATGATCCAATAATCCATTTTGCATTGGCTGAAATGTTA
    TATTATGATGGTCAATATTTACGTGCTACCTCTGAATACGAAACCGTTTTAGAAACTGGT
    GAATATCAAGTTAATGGTGTAAACTTATTCTCTCGTATGGCAGATTGTAGTTTACAAAGT
    GGTAACTATAGTGATGCGATTCGCTTATACGATGAAATTAATGAAGATGAAATGACTTCA
    GAAGATTATCTCAAAAAAGCCATTTCTTACGATAAAAATGACATCACTCAAGAAGCAATT
    AAAATAATGACTACATTACTTTCTAAAGATCCTGATTATATTCAAGGCTACTTGTATTTA
    CAATCATTATATGAAAATGAAAAAAATTATCCAGATGCCATTGAAACGGGTAAAGAAGGA
    TTACGACTAAGTCAATTTTATAAAGAGTTAATGTATACAACTGGATGTTTGGAAATAGAA
    CACGGCGATGCTAATGAAGGTGTATTATTGTTAAAACAAGCATTAGAGGTTGATAATGCT
    TACCAAGAACCTTTATTGATTTTAAGCGATTTATATCGTAATGAAGAAGATTATGAAGCA
    ATCATTGAATTATTAACATATGTAGATGAAGAAGATTTGGATCCTACATTCATGTGGCAT
    TTAGCGTTCGCCTATGGTCAAGAAGAGCGAGATAAAGAAGCTCAACATTTCTTTGAATTA
    GCATATCCAACGATGAAAACAAATATTGATTTTATGAGTGATTATTATTTCTATTTAATT
    GAAATTGGTCAAAAAGAACAAGCAATTACTATTTTAAATCAATTGTTGGAACTAGAGCCA
    AGTAACGAAAATTGGCATGACGAATTATCAAGATTGCAATCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1245

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1503 [new locus tag: SACOL_RS07655 ]
  • symbol: SACOL1503
  • description: hypothetical protein
  • length: 414
  • theoretical pI: 3.78508
  • theoretical MW: 48422.7
  • GRAVY: -0.439855

⊟Function[edit | edit source]

  • TIGRFAM:
    putative PEP-CTERM system TPR-repeat lipoprotein (TIGR02917; HMM-score: 73)
    and 8 more
    type IV pilus biogenesis/stability protein PilW (TIGR02521; HMM-score: 45.4)
    type III secretion low calcium response chaperone LcrH/SycD (TIGR02552; HMM-score: 34.6)
    tol-pal system protein YbgF (TIGR02795; HMM-score: 30.2)
    poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA (TIGR03939; HMM-score: 21.1)
    Metabolism Transport and binding proteins Amino acids, peptides and amines mitochondrial precursor proteins import receptor (TIGR00990; HMM-score: 20.5)
    Unknown function General heme biosynthesis-associated TPR protein (TIGR00540; HMM-score: 19)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking outer membrane assembly lipoprotein YfiO (TIGR03302; HMM-score: 17.3)
    pentatricopeptide repeat domain (TIGR00756; HMM-score: 13.2)
  • TheSEED  :
    • TPR domain protein
    CBSS-393130.3.peg.129  TPR-repeat-containing protein, putative component of Menaquinone-cytochrome C reductase
  • PFAM:
    TPR (CL0020) TPR_19; Tetratricopeptide repeat (PF14559; HMM-score: 79.2)
    TPR_2; Tetratricopeptide repeat (PF07719; HMM-score: 63.7)
    and 18 more
    TPR_16; Tetratricopeptide repeat (PF13432; HMM-score: 49.3)
    TPR_6; Tetratricopeptide repeat (PF13174; HMM-score: 43)
    TPR_14; Tetratricopeptide repeat (PF13428; HMM-score: 40.7)
    TPR_7; Tetratricopeptide repeat (PF13176; HMM-score: 35)
    TPR_11; TPR repeat (PF13414; HMM-score: 33)
    TPR_15; Tetratricopeptide repeat (PF13429; HMM-score: 30.7)
    ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3 (PF12895; HMM-score: 29.5)
    TPR_12; Tetratricopeptide repeat (PF13424; HMM-score: 27.7)
    TPR_8; Tetratricopeptide repeat (PF13181; HMM-score: 25.6)
    BTAD; Bacterial transcriptional activator domain (PF03704; HMM-score: 23.4)
    TPR_20; Tetratricopeptide repeat (PF14561; HMM-score: 21.7)
    TPR_17; Tetratricopeptide repeat (PF13431; HMM-score: 20.5)
    TPR_9; Tetratricopeptide repeat (PF13371; HMM-score: 20.3)
    TPR_1; Tetratricopeptide repeat (PF00515; HMM-score: 17.8)
    SGNH_hydrolase (CL0264) DUF4886; Domain of unknown function (DUF4886) (PF16227; HMM-score: 15.9)
    TPR (CL0020) SNAP; Soluble NSF attachment protein, SNAP (PF14938; HMM-score: 15)
    no clan defined MIT; MIT (microtubule interacting and transport) domain (PF04212; HMM-score: 14.8)
    TPR (CL0020) PPR; PPR repeat (PF01535; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001198
    • TAT(Tat/SPI): 0.00011
    • LIPO(Sec/SPII): 0.000243
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYNFGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLEADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETGEYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQEAIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGCLEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLDPTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITILNQLLELEPSNENWHDELSRLQS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 531 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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