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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0479 [new locus tag: NWMN_RS02740 ]
  • pan locus tag?: SAUPAN002260000
  • symbol: lysS
  • pan gene symbol?: lysS
  • synonym:
  • product: lysyl-tRNA synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0479 [new locus tag: NWMN_RS02740 ]
  • symbol: lysS
  • product: lysyl-tRNA synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 543965..545512
  • length: 1548
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTTTGAGTGTACCAGTTGGAATACTAAAGGATTACAACAAGTTAAAGGAGAGAAAGTT
    ATGTCAGAAGAAATGAATGACCAAATGTTGGTTCGACGTCAAAAATTACAAGAATTATAT
    GATCTTGGTATAGACCCGTTTGGTTCTAAATTTGACCGTTCAGGTTTATCTAGTGATTTG
    AAAGAAGAGTGGGACCAGTATTCTAAAGAAGAATTGGTAGAAAAAGAAGCGGATAGTCAT
    GTCGCTATAGCTGGACGATTAATGACTAAGCGTGGTAAAGGTAAAGCAGGATTTGCACAC
    GTTCAGGACTTAGCTGGACAAATTCAAATTTACGTTCGTAAAGATCAAGTTGGCGATGAC
    GAATTTGATTTATGGAAAAATGCTGATTTAGGCGATATCGTTGGTGTTGAAGGTGTAATG
    TTCAAAACAAATACTGGCGAATTATCGGTTAAAGCGAAGAAATTCACGCTACTAACTAAA
    TCATTGCGACCATTACCGGATAAATTCCACGGTTTACAGGATATTGAACAGAGATATCGT
    CAAAGATATTTAGATTTAATTACGAACGAAGATAGCACTCGTACATTTATTAATCGTAGT
    AAAATCATTCAAGAAATGCGTAATTATTTAAATAATAAAGGTTTCTTGGAAGTAGAAACA
    CCTATGATGCACCAAATTGCTGGTGGAGCAGCTGCTAGACCATTTGTAACACATCATAAT
    GCATTAGATGCAACGTTATACATGCGTATTGCTATTGAGTTGCATTTAAAACGTTTAATT
    GTCGGTGGACTTGAAAAAGTATATGAAATTGGTAGAGTATTCCGTAATGAAGGTGTATCA
    ACTAGACATAACCCTGAATTCACAATGATTGAATTATATGAAGCATATGCAGATTATCAT
    GACATTATGGATTTAACAGAATCTATGGTGAGACATATTGCCAATGAAGTGTTAGGTTCT
    GCAAAAGTACAATACAATGGGGAAACGATTGATTTAGAATCTGCTTGGACTCGTTTGCAT
    ATTGTTGATGCTGTAAAAGAAGCTACTGGTGTAGATTTTTATGAAGTTAAAAGTGATGAA
    GAAGCTAAAGCTTTAGCTAAAGAACATGGTATTGAAATTAAAGATACAATGAAATATGGT
    CATATTTTAAATGAATTCTTTGAGCAAAAAGTTGAAGAAACACTTATTCAGCCAACGTTT
    ATCTATGGTCATCCGACTGAAATTTCACCTTTAGCGAAGAAAAATCCTGAAGATCCTAGA
    TTTACTGATCGTTTCGAATTGTTCATTGTAGGTAGAGAGCATGCAAATGCATTTACTGAA
    TTAAATGATCCTATTGATCAAAAAGGTCGTTTTGAAGCGCAACTTGTTGAAAAAGCGCAA
    GGTAATGATGAAGCGCATGAAATGGATGAAGATTACATTGAAGCGTTAGAATATGGTATG
    CCTCCGACAGGTGGTCTTGGTATCGGTATTGACAGATTGGTTATGTTATTAACTGACTCT
    CCATCAATCAGAGACGTATTATTATTCCCTTATATGAGACAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1548

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0479 [new locus tag: NWMN_RS02740 ]
  • symbol: LysS
  • description: lysyl-tRNA synthetase
  • length: 515
  • theoretical pI: 4.95325
  • theoretical MW: 59014.5
  • GRAVY: -0.519612

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.6?  ExPASy
    Lysine--tRNA ligase ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00499; EC 6.1.1.6; HMM-score: 734.8)
    and 6 more
    Unknown function General EF-P lysine aminoacylase GenX (TIGR00462; HMM-score: 246.2)
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA ligase (TIGR00459; EC 6.1.1.-; HMM-score: 166.6)
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA(Asn) ligase (TIGR00458; EC 6.1.1.23; HMM-score: 166.4)
    Genetic information processing Protein synthesis tRNA aminoacylation asparagine--tRNA ligase (TIGR00457; EC 6.1.1.22; HMM-score: 123.8)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 28.4)
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 22)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 283.1)
    and 3 more
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 49.1)
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 24.7)
    NADP_Rossmann (CL0063) Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001762
    • TAT(Tat/SPI): 0.000616
    • LIPO(Sec/SPII): 0.000418
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFECTSWNTKGLQQVKGEKVMSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEWDQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEFDLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYRQRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFYEVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLAKKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_0500(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_2133(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_1572(rplT)50S ribosomal protein L20  [1] (data from MRSA252)
    NWMN_1549(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    NWMN_2147(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    NWMN_2150(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    NWMN_2146(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    NWMN_1613(rpsD)30S ribosomal protein S4  [1] (data from MRSA252)
    NWMN_0060(sbnA)O-Acetyl serine sulfhydrylase  [1] (data from MRSA252)
    NWMN_1155(sucC)succinyl-CoA synthetase subunit beta  [1] (data from MRSA252)
    NWMN_0641hypothetical protein  [1] (data from MRSA252)
    NWMN_0811hypothetical protein  [1] (data from MRSA252)
    NWMN_0839fumarylacetoacetate hydrolase family protein  [1] (data from MRSA252)
    NWMN_2086alkaline shock protein 23  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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