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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS02740 [old locus tag: NWMN_0479 ]
  • pan locus tag?: SAUPAN002260000
  • symbol: NWMN_RS02740
  • pan gene symbol?: lysS
  • synonym:
  • product: lysine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS02740 [old locus tag: NWMN_0479 ]
  • symbol: NWMN_RS02740
  • product: lysine--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 544025..545512
  • length: 1488
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (544025..545512) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0479

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTCAGAAGAAATGAATGACCAAATGTTGGTTCGACGTCAAAAATTACAAGAATTATAT
    GATCTTGGTATAGACCCGTTTGGTTCTAAATTTGACCGTTCAGGTTTATCTAGTGATTTG
    AAAGAAGAGTGGGACCAGTATTCTAAAGAAGAATTGGTAGAAAAAGAAGCGGATAGTCAT
    GTCGCTATAGCTGGACGATTAATGACTAAGCGTGGTAAAGGTAAAGCAGGATTTGCACAC
    GTTCAGGACTTAGCTGGACAAATTCAAATTTACGTTCGTAAAGATCAAGTTGGCGATGAC
    GAATTTGATTTATGGAAAAATGCTGATTTAGGCGATATCGTTGGTGTTGAAGGTGTAATG
    TTCAAAACAAATACTGGCGAATTATCGGTTAAAGCGAAGAAATTCACGCTACTAACTAAA
    TCATTGCGACCATTACCGGATAAATTCCACGGTTTACAGGATATTGAACAGAGATATCGT
    CAAAGATATTTAGATTTAATTACGAACGAAGATAGCACTCGTACATTTATTAATCGTAGT
    AAAATCATTCAAGAAATGCGTAATTATTTAAATAATAAAGGTTTCTTGGAAGTAGAAACA
    CCTATGATGCACCAAATTGCTGGTGGAGCAGCTGCTAGACCATTTGTAACACATCATAAT
    GCATTAGATGCAACGTTATACATGCGTATTGCTATTGAGTTGCATTTAAAACGTTTAATT
    GTCGGTGGACTTGAAAAAGTATATGAAATTGGTAGAGTATTCCGTAATGAAGGTGTATCA
    ACTAGACATAACCCTGAATTCACAATGATTGAATTATATGAAGCATATGCAGATTATCAT
    GACATTATGGATTTAACAGAATCTATGGTGAGACATATTGCCAATGAAGTGTTAGGTTCT
    GCAAAAGTACAATACAATGGGGAAACGATTGATTTAGAATCTGCTTGGACTCGTTTGCAT
    ATTGTTGATGCTGTAAAAGAAGCTACTGGTGTAGATTTTTATGAAGTTAAAAGTGATGAA
    GAAGCTAAAGCTTTAGCTAAAGAACATGGTATTGAAATTAAAGATACAATGAAATATGGT
    CATATTTTAAATGAATTCTTTGAGCAAAAAGTTGAAGAAACACTTATTCAGCCAACGTTT
    ATCTATGGTCATCCGACTGAAATTTCACCTTTAGCGAAGAAAAATCCTGAAGATCCTAGA
    TTTACTGATCGTTTCGAATTGTTCATTGTAGGTAGAGAGCATGCAAATGCATTTACTGAA
    TTAAATGATCCTATTGATCAAAAAGGTCGTTTTGAAGCGCAACTTGTTGAAAAAGCGCAA
    GGTAATGATGAAGCGCATGAAATGGATGAAGATTACATTGAAGCGTTAGAATATGGTATG
    CCTCCGACAGGTGGTCTTGGTATCGGTATTGACAGATTGGTTATGTTATTAACTGACTCT
    CCATCAATCAGAGACGTATTATTATTCCCTTATATGAGACAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1488

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS02740 [old locus tag: NWMN_0479 ]
  • symbol: NWMN_RS02740
  • description: lysine--tRNA ligase
  • length: 495
  • theoretical pI: 4.89349
  • theoretical MW: 56718.8
  • GRAVY: -0.512929

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.6?  ExPASy
    Lysine--tRNA ligase ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00499; EC 6.1.1.6; HMM-score: 735)
    and 6 more
    Unknown function General EF-P lysine aminoacylase GenX (TIGR00462; HMM-score: 246.3)
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA ligase (TIGR00459; EC 6.1.1.-; HMM-score: 166.9)
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA(Asn) ligase (TIGR00458; EC 6.1.1.23; HMM-score: 166.6)
    Genetic information processing Protein synthesis tRNA aminoacylation asparagine--tRNA ligase (TIGR00457; EC 6.1.1.22; HMM-score: 124)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 28.5)
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 22.2)
  • TheSEED: see NWMN_0479
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 318.1)
    and 2 more
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 56.5)
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 24.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9917
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0081
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002378
    • TAT(Tat/SPI): 0.000871
    • LIPO(Sec/SPII): 0.000431
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEWDQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEFDLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYRQRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFYEVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLAKKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_RS00340siderophore biosynthesis protein SbnA  [1] (data from MRSA252)
    NWMN_RS0291550S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_RS03640LysR family transcriptional regulator  [1] (data from MRSA252)
    NWMN_RS04590NADH dehydrogenase  [1] (data from MRSA252)
    NWMN_RS04730hypothetical protein  [1] (data from MRSA252)
    NWMN_RS05380pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    NWMN_RS0649050S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_RS06515succinyl-CoA ligase subunit beta  [1] (data from MRSA252)
    NWMN_RS0868550S ribosomal protein L21  [1] (data from MRSA252)
    NWMN_RS0882050S ribosomal protein L20  [1] (data from MRSA252)
    NWMN_RS08930pyruvate kinase  [1] (data from MRSA252)
    NWMN_RS0904530S ribosomal protein S4  [1] (data from MRSA252)
    NWMN_RS12080Asp23/Gls24 family envelope stress response protein  [1] (data from MRSA252)
    NWMN_RS1233050S ribosomal protein L15  [1] (data from MRSA252)
    NWMN_RS1239530S ribosomal protein S3  [1] (data from MRSA252)
    NWMN_RS1240050S ribosomal protein L22  [1] (data from MRSA252)
    NWMN_RS1241550S ribosomal protein L23  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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