PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002337
• pan locus tag^?: SAUPAN005883000
• symbol: JSNZ_002337
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002337
• symbol: JSNZ_002337
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2336050..2336655
• length: 606
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  
  ATGAAAAGATTAGTTACAGGGTTACTAGCATTATCATTATTTTTAGCTGCATGTGGTCAA
  GATAGTGACCAACAAAAAGACGGTAATAAAGAAAAAGATGATAAAGCGAAAACTGAACAA
  CAAGATAAAAAAACAAATGATTCATCTAAAGATAAGAAAGATAATAAAGATGATAGTAAA
  GACGTAAACAACGATAACCAGCAACAATCTAATTCAAATGCAACAAACAATGACCAAAAC
  CAAACAAATAATAACCAATCAAGTAATAACCAAGCGAATAATAATCAAAAATCAAGTTAC
  GTTGCACCATATTATGGACAAAATGCCGCGCCGGTTGCACGTCAAATTTATCCGTTTAAT
  GGAAATAAAACTCAAGCTTTACAGCAATTGCCAAATTTCCAAACAGCTTTAAATGCGGCT
  AATAATGAAGCAAATAAATTTGGTAGTAATAATAAAGTGTATAATGATTATTCTATTGAA
  GAACATAATGGCAACTATAAGTATGTGTTTAGTTTTAAAGACCCAAATGCAAATGGAAAA
  TATTCAATTGTAACGGTTGATTATACTGGACAAGCAATGGTTACTGATCCAAACTACCAA
  CAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  606
  

⊟Protein

⊟General

• locus tag: JSNZ_002337
• symbol: JSNZ_002337
• description: hypothetical protein
• length: 201
• theoretical pI: 5.95757
• theoretical MW: 22475.9
• GRAVY: -1.3806

⊟Function

• TIGRFAM:
  Sec region non-globular protein (TIGR04420; HMM-score: 16.3)
  and 4 more
  cobaltochelatase subunit (TIGR02442; EC 6.6.1.2; HMM-score: 9.7)
  Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 8.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobaltochelatase, CobT subunit (TIGR01651; EC 6.6.1.2; HMM-score: 8.2)
  Metabolism Transport and binding proteins Cations and iron carrying compounds potassium uptake protein, Trk family (TIGR00934; HMM-score: 5.4)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  AhpD-like (CL0423) PA26; PA26 p53-induced protein (sestrin) (PF04636; HMM-score: 14.9)
  no clan defined Nop14; Nop14-like family (PF04147; HMM-score: 14.4)
  HAD (CL0137) CDC45; CDC45 (PF02724; HMM-score: 14.1)
  no clan defined PCYCGC; Protein of unknown function with PCYCGC motif (PF13798; HMM-score: 13)
  Piezo_TM1-24; Piezo TM1-24 (PF24871; HMM-score: 12.9)
  LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 12)
  and 15 more
  no clan defined SLC12; Solute carrier family 12 (PF03522; HMM-score: 10.6)
  Peptidase_AD (CL0130) Presenilin; Presenilin (PF01080; HMM-score: 10.4)
  no clan defined DUF6474; Family of unknown function (DUF6474) (PF20079; HMM-score: 10.2)
  MFS (CL0015) CLN3; CLN3 protein (PF02487; HMM-score: 9.6)
  DMT (CL0184) Zip; ZIP Zinc transporter (PF02535; HMM-score: 9.1)
  GPCR_A (CL0192) SID-1_RNA_chan; dsRNA-gated channel SID-1 (PF13965; HMM-score: 8.9)
  no clan defined TMEM51; Transmembrane protein 51 (PF15345; HMM-score: 8.3)
  MCM_bind; Mini-chromosome maintenance replisome factor (PF09739; HMM-score: 8.2)
  FUSC (CL0307) ALMT; Aluminium activated malate transporter (PF11744; HMM-score: 8.1)
  no clan defined FAM176; FAM176 family (PF14851; HMM-score: 8.1)
  NPR (CL0435) NPR3; Nitrogen Permease regulator of amino acid transport activity 3 (PF03666; HMM-score: 7.9)
  no clan defined CobT; Cobalamin biosynthesis protein CobT (PF06213; HMM-score: 7.9)
  Mat89Bb; Cell cycle and development regulator Mat89Bb (PF10221; HMM-score: 6.9)
  Otopetrin; Otopetrin (PF03189; HMM-score: 6.4)
  Raftlin; Raftlin (PF15250; HMM-score: 5.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.87
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0002
  • Cytoplasmic Membrane Score: 0.812
  • Cell wall & surface Score: 0.0351
  • Extracellular Score: 0.1528
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
  • SP(Sec/SPI): 0.000417
  • TAT(Tat/SPI): 0.00006
  • LIPO(Sec/SPII): 0.99935
  • Cleavage Site: CS pos: 17-18. LAA-CG. Pr: 0.9998
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKKTNDSSKDKKDNKDDSKDVNNDNQQQSNSNATNNDQNQTNNNQSSNNQANNNQKSSYVAPYYGQNAAPVARQIYPFNGNKTQALQQLPNFQTALNAANNEANKFGSNNKVYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SigB (activation) regulon

  SigB

  (sigma factor)

  controlling a large regulon involved in stress/starvation response and adaptation; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)   other strains

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications