PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002014
• pan locus tag^?: SAUPAN005308000
• symbol: leuB
• pan gene symbol^?: leuB
• synonym:
• product: 3-isopropylmalate dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002014
• symbol: leuB
• product: 3-isopropylmalate dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 2028017..2029063
• length: 1047
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGA
  TCTCTATCATTGCTTGAAATTATAAGAAATAAATATAACTTTAATTATCAAATAGAGCAC
  CACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTA
  AATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACA
  GATCCTAACAATCGACCAGAACAAGGATTATTAAAATTGCGTAAATCCTTAAATTTATTT
  GCAAATATACGCCCCACTACCGTTGTCAAAGGCGCTAGTTCTTTATCACCTTTAAAGGAA
  GAACGCGTTGAAGGCACAGATTTAGTTATAGTCCGTGAATTGACAAGTGGTATTTATTTT
  GGAGAACCTAGACATTTTAATAATCACGAGGCCTTAGATTCTCTTACTTATACAAGAGAA
  GAAATAGAACGCATTGTTCACGTAGCATTTAAATTGGCCGCTTCAAGACGAGGAAAACTA
  ACATCAGTTGATAAAGAAAATGTATTAGCTTCTAGTAAATTGTGGCGCAAAGTCGTAAAT
  GAAGTAAGTCAATTATATCCAGAAGTAACAGTAAATCACTTACTTGTTGATGCTTGTAGT
  ATGCATTTAATCACAAATCCAAAACAATTTGACGTCATCGTATGTGAAAACTTATTTGGC
  GATATTTTAAGTGATGAAGCTTCAGTGATTCCTGGTTCACTTGGTTTATCACCTTCTGCT
  AGTTTTAGTAACGATGGTCCAAGATTGTATGAGCCTATTCATGGATCAGCACCAGATATT
  GCAGGTAAAAACGTTGCCAATCCATTTGGAATGATTCTATCTTTAGCGATGTGTTTACGT
  GAAAGCTTAAATCAACCAGATGCTGCAGATGAATTAGAACAACATATTTATAGCATGATT
  GAACATGGGCAAACGACAGCAGATTTAGGCGGCAAATTGAATACTACTGATATTTTCGAA
  ATTCTATCTCAAAAATTGAATCACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1047
  

⊟Protein

⊟General

• locus tag: JSNZ_002014
• symbol: LeuB
• description: 3-isopropylmalate dehydrogenase
• length: 348
• theoretical pI: 5.40205
• theoretical MW: 38254.2
• GRAVY: -0.191092

⊟Function

• reaction:
  EC 1.1.1.85?  ExPASy
  3-isopropylmalate dehydrogenase (2R,3S)-3-isopropylmalate + NAD⁺ = 4-methyl-2-oxopentanoate + CO₂ + NADH
• TIGRFAM:
  Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydrogenase (TIGR00169; EC 1.1.1.85; HMM-score: 472.5)
  and 6 more
  isopropylmalate/isohomocitrate dehydrogenases (TIGR02088; HMM-score: 295.3)
  Metabolism Energy metabolism Other tartrate dehydrogenase (TIGR02089; EC 1.1.1.93; HMM-score: 243.7)
  Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase (TIGR02924; EC 1.1.1.-; HMM-score: 185.1)
  Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase, NAD-dependent (TIGR00175; EC 1.1.1.41; HMM-score: 179.7)
  Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase, NADP-dependent (TIGR00183; EC 1.1.1.42; HMM-score: 106.5)
  Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase, NADP-dependent (TIGR00127; EC 1.1.1.42; HMM-score: 11.2)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Iso_DH (CL0270) Iso_dh; Isocitrate/isopropylmalate dehydrogenase (PF00180; HMM-score: 438.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9949
  • Cytoplasmic Membrane Score: 0.0006
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0045
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002426
  • TAT(Tat/SPI): 0.000194
  • LIPO(Sec/SPII): 0.000376
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MTYNIVALPGDGIGPEILNGSLSLLEIIRNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFANIRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREEIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLNTTDIFEILSQKLNH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications