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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2011 [new locus tag: SAUSA300_RS11060 ]
- pan locus tag?: SAUPAN005308000
- symbol: leuB
- pan gene symbol?: leuB
- synonym:
- product: 3-isopropylmalate dehydrogenase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2011 [new locus tag: SAUSA300_RS11060 ]
- symbol: leuB
- product: 3-isopropylmalate dehydrogenase
- replicon: chromosome
- strand: +
- coordinates: 2171315..2172361
- length: 1047
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3915080 NCBI
- RefSeq: YP_494662 NCBI
- BioCyc: see SAUSA300_RS11060
- MicrobesOnline: 1293526 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1021ATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGA
TCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCAC
CACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTA
AATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACA
GATCCTAACAATCGACCAGAACAAGGATTATTAAAATTGCGTAAATCCTTAAATTTATTT
GTAAATATACGCCCCACTACCGTTGTCAAAGGCGCTAGTTCTTTATCACCTTTAAAGGAA
GAACGCGTTGAAGGCACAGATTTAGTTATAGTCCGTGAATTGACAAGTGGTATTTATTTT
GGAGAACCTAGACATTTTAATAATCACGAGGCCTTAGATTCTCTTACTTATACAAGAGAA
GAAATAGAACGCATTGTTCACGTAGCATTTAAATTGGCCGCTTCAAGACGAGGAAAACTA
ACATCAGTTGATAAAGAAAATGTATTAGCTTCTAGTAAATTGTGGCGCAAAGTCGTAAAT
GAAGTAAGTCAATTATATCCAGAAGTAACAGTAAATCACTTACTTGTTGATGCTTGTAGT
ATGCATTTAATCACAAATCCAAAACAATTTGACGTCATCGTATGTGAAAACTTATTTGGC
GATATTTTAAGTGATGAAGCTTCAGTGATTCCTGGTTCACTTGGTTTATCACCTTCTGCT
AGTTTTAGTAACGATGGTCCAAGATTGTATGAGCCTATTCATGGATCAGCACCAGATATT
GCAGGTAAAAACGTTGCCAATCCATTTGGAATGATTCTATCTTTAGCGATGTGTTTACGT
GAAAGCTTAAATCAACCAGATGCTGCAGATGAATTAGAACAACATATTTATAGCATGATT
GAACATGGGCAAACGACAGCAGATTTAGGCGGCAAATTGAATACTACTGATATTTTCGAA
ATTCTATCTCAAAAATTGAATCACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2011 [new locus tag: SAUSA300_RS11060 ]
- symbol: LeuB
- description: 3-isopropylmalate dehydrogenase
- length: 348
- theoretical pI: 5.24197
- theoretical MW: 38213.1
- GRAVY: -0.173563
⊟Function[edit | edit source]
- reaction: EC 1.1.1.85? ExPASy3-isopropylmalate dehydrogenase (2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH
- TIGRFAM: Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydrogenase (TIGR00169; EC 1.1.1.85; HMM-score: 470.2)and 6 moreisopropylmalate/isohomocitrate dehydrogenases (TIGR02088; HMM-score: 292.5)Energy metabolism Other tartrate dehydrogenase (TIGR02089; EC 1.1.1.93; HMM-score: 244.7)Energy metabolism TCA cycle isocitrate dehydrogenase (TIGR02924; EC 1.1.1.-; HMM-score: 182.2)Energy metabolism TCA cycle isocitrate dehydrogenase, NAD-dependent (TIGR00175; EC 1.1.1.41; HMM-score: 178.4)Energy metabolism TCA cycle isocitrate dehydrogenase, NADP-dependent (TIGR00183; EC 1.1.1.42; HMM-score: 108)Energy metabolism TCA cycle isocitrate dehydrogenase, NADP-dependent (TIGR00127; EC 1.1.1.42; HMM-score: 11.2)
- TheSEED :
- 3-isopropylmalate dehydrogenase (EC 1.1.1.85)
Amino Acids and Derivatives Branched-chain amino acids Branched-Chain Amino Acid Biosynthesis 3-isopropylmalate dehydrogenase (EC 1.1.1.85)and 1 more - PFAM: Iso_DH (CL0270) Iso_dh; Isocitrate/isopropylmalate dehydrogenase (PF00180; HMM-score: 410.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Mg2+, Mn2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003514
- TAT(Tat/SPI): 0.000203
- LIPO(Sec/SPII): 0.000502
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVNIRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREEIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLNTTDIFEILSQKLNH
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Charlotte D Majerczyk, Paul M Dunman, Thanh T Luong, Chia Y Lee, Marat R Sadykov, Greg A Somerville, Kip Bodi, Abraham L Sonenshein
Direct targets of CodY in Staphylococcus aureus.
J Bacteriol: 2010, 192(11);2861-77
[PubMed:20363936] [WorldCat.org] [DOI] (I p)