PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS11060 [old locus tag: SAUSA300_2011 ]
• pan locus tag^?: SAUPAN005308000
• symbol: SAUSA300_RS11060
• pan gene symbol^?: leuB
• synonym:
• product: 3-isopropylmalate dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS11060 [old locus tag: SAUSA300_2011 ]
• symbol: SAUSA300_RS11060
• product: 3-isopropylmalate dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 2171315..2172361
• length: 1047
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2171315..2172361) NCBI
• BioCyc: SAUSA300_RS11060 BioCyc
• MicrobesOnline: see SAUSA300_2011

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
  
  ATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGA
  TCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCAC
  CACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTA
  AATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACA
  GATCCTAACAATCGACCAGAACAAGGATTATTAAAATTGCGTAAATCCTTAAATTTATTT
  GTAAATATACGCCCCACTACCGTTGTCAAAGGCGCTAGTTCTTTATCACCTTTAAAGGAA
  GAACGCGTTGAAGGCACAGATTTAGTTATAGTCCGTGAATTGACAAGTGGTATTTATTTT
  GGAGAACCTAGACATTTTAATAATCACGAGGCCTTAGATTCTCTTACTTATACAAGAGAA
  GAAATAGAACGCATTGTTCACGTAGCATTTAAATTGGCCGCTTCAAGACGAGGAAAACTA
  ACATCAGTTGATAAAGAAAATGTATTAGCTTCTAGTAAATTGTGGCGCAAAGTCGTAAAT
  GAAGTAAGTCAATTATATCCAGAAGTAACAGTAAATCACTTACTTGTTGATGCTTGTAGT
  ATGCATTTAATCACAAATCCAAAACAATTTGACGTCATCGTATGTGAAAACTTATTTGGC
  GATATTTTAAGTGATGAAGCTTCAGTGATTCCTGGTTCACTTGGTTTATCACCTTCTGCT
  AGTTTTAGTAACGATGGTCCAAGATTGTATGAGCCTATTCATGGATCAGCACCAGATATT
  GCAGGTAAAAACGTTGCCAATCCATTTGGAATGATTCTATCTTTAGCGATGTGTTTACGT
  GAAAGCTTAAATCAACCAGATGCTGCAGATGAATTAGAACAACATATTTATAGCATGATT
  GAACATGGGCAAACGACAGCAGATTTAGGCGGCAAATTGAATACTACTGATATTTTCGAA
  ATTCTATCTCAAAAATTGAATCACTAA
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  1047
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS11060 [old locus tag: SAUSA300_2011 ]
• symbol: SAUSA300_RS11060
• description: 3-isopropylmalate dehydrogenase
• length: 348
• theoretical pI: 5.24197
• theoretical MW: 38213.1
• GRAVY: -0.173563

⊟Function

• reaction:
  EC 1.1.1.85?  ExPASy
  3-isopropylmalate dehydrogenase (2R,3S)-3-isopropylmalate + NAD⁺ = 4-methyl-2-oxopentanoate + CO₂ + NADH
• TIGRFAM:
  Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydrogenase (TIGR00169; EC 1.1.1.85; HMM-score: 470.2)
  and 6 more
  isopropylmalate/isohomocitrate dehydrogenases (TIGR02088; HMM-score: 292.5)
  Metabolism Energy metabolism Other tartrate dehydrogenase (TIGR02089; EC 1.1.1.93; HMM-score: 244.7)
  Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase (TIGR02924; EC 1.1.1.-; HMM-score: 182.2)
  Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase, NAD-dependent (TIGR00175; EC 1.1.1.41; HMM-score: 178.4)
  Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase, NADP-dependent (TIGR00183; EC 1.1.1.42; HMM-score: 108)
  Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase, NADP-dependent (TIGR00127; EC 1.1.1.42; HMM-score: 11.2)
• TheSEED: see SAUSA300_2011
• PFAM:
  Iso_DH (CL0270) Iso_dh; Isocitrate/isopropylmalate dehydrogenase (PF00180; HMM-score: 410.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺, Mn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003514
  • TAT(Tat/SPI): 0.000203
  • LIPO(Sec/SPII): 0.000502
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446144103 NCBI
• RefSeq: WP_000221958 NCBI
• UniProt: see SAUSA300_2011

⊟Protein sequence

• MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVNIRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREEIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLNTTDIFEILSQKLNH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SAUSA300_2011

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications