From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002015
  • pan locus tag?: SAUPAN005309000
  • symbol: leuC
  • pan gene symbol?: leuC
  • synonym:
  • product: 3-isopropylmalate dehydratase large subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002015
  • symbol: leuC
  • product: 3-isopropylmalate dehydratase large subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 2029077..2030447
  • length: 1371
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCAAACATTATTTGACAAGGTGTGGAACAGACATGTGTTATACGGGAAATTGGGC
    GAACCGCAACTATTATACATTGATTTACACCTTATACATGAAGTTACTTCTCCTCAAGCA
    TTTGAAGGACTTAGGCTTCAAAACAGAAAATTAAGACGCCCAGATTTAACATTTGCAACA
    CTCGATCACAATGTTCCTACTATTGATATTTTCAATATTAAAGATGAAATTGCAAACAAA
    CAAATCACAACATTACAAAAAAACGCCATAGATTTTGGGGTGCATATTTTTGATATGGGC
    TCTGATGAACAAGGTATTGTTCACATGGTAGGACCTGAGACAGGACTTACACAGCCCGGC
    AAAACAATCGTTTGTGGTGACTCTCACACAGCAACACATGGAGCATTTGGTGCTATTGCA
    TTTGGAATTGGAACAAGTGAAGTTGAACATGTTTTCGCAACTCAAACGCTATGGCAAACA
    AAACCCAAAAACTTAAAAATCGATATTAATGGTACCTTACCAACAGGCGTCTATGCTAAG
    GACATTATTCTGCATTTAATTAAAACGTATGGTGTTGATTTTGGTACAGGCTATGCTTTG
    GAATTTACTGGCGAAACAATTAAAAACCTTTCAATGGACGGTCGAATGACTATTTGTAAC
    ATGGCTATCGAAGGTGGTGCCAAATACGGCATAATCCAACCTGATGATATAACATTTGAA
    TATGTTAAAGGGAGACCATTTGCCGATAACTTCGCTAAATCAGTTGATAAGTGGCGTGAG
    CTATATTCTGATGACGACGCGATATTTGATCGTGTAATTGAACTTGATGTTTCAACATTA
    GAACCACAAGTGACATGGGGAACTAATCCTGAAATGGGTGTTAATTTCAGTGAACCATTC
    CCTGAAATCAAAGATATCAACGATCAACGTGCGTATGATTATATGGGGTTAGAACCAGGT
    CAAAAAGCTGAAGACATCGACTTAGGGTATGTTTTTCTCGGTTCATGTACAAATGCTAGA
    CTATCAGATTTGATTGAAGCTAGTCATATTGTTAAAGGAAATAAAGTTCATCCAAATATT
    ACAGCTATTGTCGTACCAGGTTCTCGTACAGTAAAAAAAGAAGCAGAAAAATTAGGTCTA
    GATACTATCTTTAAAAATGCAGGATTTGAATGGCGTGAACCAGGATGTTCAATGTGTTTA
    GGCATGAATCCTGACCAAGTACCTGAGGGCGTACATTGTGCATCTACAAGTAATCGAAAC
    TTTGAAGGACGACAAGGCAAAGGTGCAAGAACACATTTAGTATCCCCTGCTATGGCAGCA
    GCAGCAGCTATTCATGGTAAATTTGTGGACGTAAGAAAGGTGGTTGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1371

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002015
  • symbol: LeuC
  • description: 3-isopropylmalate dehydratase large subunit
  • length: 456
  • theoretical pI: 5.71556
  • theoretical MW: 50339.9
  • GRAVY: -0.23114

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.33?  ExPASy
    3-isopropylmalate dehydratase (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, large subunit (TIGR00170; EC 4.2.1.33; HMM-score: 696.2)
    and 9 more
    homoaconitate hydratase family protein (TIGR01343; HMM-score: 340.7)
    3-isopropylmalate dehydratase, large subunit (TIGR02086; HMM-score: 305.7)
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, large subunit (TIGR02083; EC 4.2.1.33; HMM-score: 287.2)
    Metabolism Energy metabolism TCA cycle putative aconitate hydratase (TIGR01342; EC 4.2.1.3; HMM-score: 206.5)
    Metabolism Amino acid biosynthesis Aspartate family homoaconitase (TIGR00139; EC 4.2.1.36; HMM-score: 151.6)
    Metabolism Energy metabolism TCA cycle aconitate hydratase, mitochondrial (TIGR01340; EC 4.2.1.3; HMM-score: 145.4)
    Metabolism Energy metabolism TCA cycle aconitate hydratase 1 (TIGR01341; EC 4.2.1.3; HMM-score: 105.7)
    2-methylisocitrate dehydratase, Fe/S-dependent (TIGR02333; EC 4.2.1.99; HMM-score: 91.3)
    Metabolism Energy metabolism TCA cycle aconitate hydratase 2 (TIGR00117; EC 4.2.1.3; HMM-score: 71.6)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined Aconitase; Aconitase family (aconitate hydratase) (PF00330; HMM-score: 561.1)
    and 1 more
    AcnX; Aconitase X (PF04412; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9805
    • Cytoplasmic Membrane Score: 0.0059
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0136
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001455
    • TAT(Tat/SPI): 0.000137
    • LIPO(Sec/SPII): 0.000258
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGLRLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSDEQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQTKPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTICNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELDVSTLEPQVTWGTNPEMGVNFSEPFPEIKDINDQRAYDYMGLEPGQKAEDIDLGYVFLGSCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWREPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVDVRKVVV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_002015&oldid=107656"