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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002013
  • pan locus tag?: SAUPAN005307000
  • symbol: JSNZ_002013
  • pan gene symbol?: leuA
  • synonym:
  • product: 2-isopropylmalate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002013
  • symbol: JSNZ_002013
  • product: 2-isopropylmalate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 2026485..2028014
  • length: 1530
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGTAGTCATATTCAAATTTTTGATACGACACTAAGAGACGGTGAACAAACACCAGGA
    GTGAATTTTACTTTTGATGAACGCTTGCGTATTGCATTGCAATTAGAAAAATGGGGTGTA
    GATGTTATTGAAGCTGGATTTCCTGCTTCAAGTACAGGTAGCTTTAAATCTGTTCAAGCA
    ATTGCACAAACATTAACAACAACGGCTGTATGTGGTTTAGCTAGATGTAAAAAATCTGAC
    ATCGATGCTGTATATGAAGCAACAAAAGATGCAGCGAAGCCGGTCGTGCATGTTTTTATA
    GCAACATCACCTATTCATCTTGAACATAAACTTAAAATGTCTCAAGAAGACGTTTTAGCA
    TCTATTAAAGAACATGTCACATACGCGAAACAATTATTTGACGTTGTTCAATTTTCACCT
    GAAGATGCAACGCGTACTGAATTACCATTCTTAGTGAAATGTGTACAAACTGCCGTTGAC
    GCTGGAGCTACAGTTATTAATATTCCTGATACAGTCGGCTACAGTTACCATGATGAATAT
    GCACATATTTTCAAAACCTTAACAGAATCTGTAACATCTTCAAATGAAATTATTTATAGT
    GCTCATTGCCATGACGATTTAGGAATGGCTGTTTCAAATAGTTTAGCTGCAATTGAAGGC
    GGTGCGAGACGAATTGAAGGCACTGTAAATGGTATTGGTGAACGAGCAGGTAATGCAGCA
    CTTGAAGAAGTCGCGCTTGCACTATACGTTCGAAATGATCATTATGGTGCTCAAACTGCT
    CTTAATCTCGAAGAAACTAAAAAAACATCGGATTTAATTTCAAGATATGCAGGTATTCGA
    GTGCCTAGAAATAAAGCAATTGTTGGCCAAAATGCATTTAGTCATGAATCAGGTATTCAC
    CAAGATGGCGTATTAAAACATCGTGAAACATATGAAATTATGACACCTCAACTTGTTGGT
    GTAAGCACGACTGAACTTCCATTAGGAAAATTATCTGGTAAACACGCCTTCTCAGAGAAG
    TTAAAAGCATTAGGTTATGACATTGATAAAGAAGCGCAAATAGATTTATTTAAACAATTC
    AAGGCCATTGCGGACAAAAAGAAATCTGTTTCAGATAGAGATATTCATGCGATTATTCAA
    GGTTCTGAGCATGAGCATCAAGCACTTTATAAATTGGAAACACTGCAACTACAATATGTC
    TCTAGCGGCCTTCAAAGTGCTGTTGTTGTTGTTAAAGATAAAGAGGGTCATATTTACCAG
    GATTCAAGTATTGGTACTGGCTCAATCGTAGCAATTTACAATGCAGTTGATCGTATTTTC
    CAGAAAGAAACAGAATTAATTGATTATCGTATTAATTCTGTCACTGAAGGTACTGATGCC
    CAAGCAGAAGTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATT
    GATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCA
    GCTGAAAATGTTGAAAAGGTAGGTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1530

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002013
  • symbol: JSNZ_002013
  • description: 2-isopropylmalate synthase
  • length: 509
  • theoretical pI: 5.75975
  • theoretical MW: 55674.3
  • GRAVY: -0.198821

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.3.13?  ExPASy
    2-isopropylmalate synthase Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00973; EC 2.3.3.13; HMM-score: 662.6)
    and 9 more
    isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 405)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 315.2)
    Metabolism Central intermediary metabolism Nitrogen fixation homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 315.2)
    homocitrate synthase (TIGR02146; EC 2.3.3.14; HMM-score: 282.4)
    Unknown function General citramalate synthase (TIGR00977; EC 2.3.1.182; HMM-score: 207.9)
    Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00970; EC 2.3.3.13; HMM-score: 128)
    Metabolism Energy metabolism Other 4-hydroxy-2-oxovalerate aldolase (TIGR03217; EC 4.1.3.39; HMM-score: 75.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 15.1)
    Metabolism Energy metabolism Other oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 15.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) HMGL-like; HMGL-like (PF00682; HMM-score: 310.2)
    and 3 more
    no clan defined LeuA_dimer; LeuA allosteric (dimerisation) domain (PF08502; HMM-score: 126.3)
    Post-HMGL (CL0597) HCS_D2; Homocitrate synthase post-HMGL domain-like (PF22617; HMM-score: 95.8)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 10.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9644
    • Cytoplasmic Membrane Score: 0.0124
    • Cell wall & surface Score: 0.0018
    • Extracellular Score: 0.0214
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005044
    • TAT(Tat/SPI): 0.002612
    • LIPO(Sec/SPII): 0.000366
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVDAGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGKHAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVGN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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