PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001811
• pan locus tag^?: SAUPAN004567000
• symbol: splA
• pan gene symbol^?: splA
• synonym:
• product: serine protease SplA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001811
• symbol: splA
• product: serine protease SplA
• replicon: chromosome
• strand: -
• coordinates: 1857403..1858110
• length: 708
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  
  ATGAATAAAAATGTAATGGTTAAAGGTTTAACTGCTTTAACTATTTTAACATCTCTTGGT
  TTCGCTGAAAATATTTCTAATCAGCCTCATTCAATTGCCAAAGCAGAAAAGAATGTCAAA
  GAAATTACCGATGCAACTAAGGCACCATACAATTCAGTGGTAGCATTTGCGGGTGGTACT
  GGTGTAGTTGTTGGTAAAAATACAATCGTAACTAACAAACATATCGCTAAAAGTAATAAT
  ATTTTTAAAAATAGAGTAGCAGCACATCATTCGAGCAAAGGTAAAGGCGGAGGAAACTAC
  GACGTTAAAGACATTGTAGAATATCCTGGAAAAGAAGACCTTGCGATAGTTCATGTTCAT
  GAAACAAGTACAGAAGGTTTGAATTTTAATAAGAACGTTAGTTATACAAAGTTTGCAGAC
  GGAGCAAAAGCGAAAGATAGAATTTCTGTTATTGGTTATCCAAAGGGTGCACAAACCAAA
  TATAAAATGTTTGAATCAACTGGAACGATTAACCATATCAATGGAACGTTTATGGAATTT
  GATGCGTATGCACAACCAGGGAACTCTGGATCACCTGTATTGAATTCTCATAATGAACTG
  ATTGGTATTTTATATGCAGGTAGTGGAAAAGATGAATCTGAAAAGAATTTCGGTGTTTAC
  TTCACACCACAATTAAAAGAATTTATTCAAAATAATATTGAAAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  708
  

⊟Protein

⊟General

• locus tag: JSNZ_001811
• symbol: SplA
• description: serine protease SplA
• length: 235
• theoretical pI: 9.65954
• theoretical MW: 25430.5
• GRAVY: -0.42766

⊟Function

• reaction:
  EC 3.4.21.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 25.5)
  Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 25.5)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 21.1)
  Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 21.1)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 78.7)
  Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 70)
  and 4 more
  Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 23.1)
  Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 20.1)
  Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 18.9)
  Mycop_pep_DUF31; Mycoplasma peptidase (DUF31) (PF01732; HMM-score: 17.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.73
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cell wall & surface Score: 0.0012
  • Extracellular Score: 0.9988
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 35 aa
  • SP(Sec/SPI): 0.916215
  • TAT(Tat/SPI): 0.013109
  • LIPO(Sec/SPII): 0.019625
  • Cleavage Site: CS pos: 35-36. AKA-EK. Pr: 0.5721
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITDATKAPYNSVVAFAGGTGVVVGKNTIVTNKHIAKSNNIFKNRVAAHHSSKGKGGGNYDVKDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKAKDRISVIGYPKGAQTKYKMFESTGTINHINGTFMEFDAYAQPGNSGSPVLNSHNELIGILYAGSGKDESEKNFGVYFTPQLKEFIQNNIEK

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR (activation) regulon

  SaeR

  (TF)

  important in Virulence; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications