PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001808
• pan locus tag^?: SAUPAN004564000
• symbol: splC
• pan gene symbol^?: splC
• synonym:
• product: serine protease SplC

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001808
• symbol: splC
• product: serine protease SplC
• replicon: chromosome
• strand: -
• coordinates: 1854999..1855718
• length: 720
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  
  ATGAATAAAAACGTAGTTATTAAAAGTATTGCAGCTTTGACCATATTAACATCTATAACT
  GGCATAGGTGATTCAGTCCGAGAAGAAAAACAACAAATAGCGAAAGCTGAGAAAAATGTT
  ACACAAGTTAAAGATACCAATGTCTCTCCATATAAAGGTGTTGTGGCATTTAAAGATGCG
  ACTGGTTTTGTGATTGGTAAAAATACCATTATCACTAATAAACATGTATCGAAAGATTAC
  AAAGTGGGTGATCGAATTACCGCGCACCCAAATGGCGACAAAGGCAACGGTGGCATTTAT
  AAAATTAAAAATATTTCTGATTATCCAGATAATGAGGATATATCAGTAATGAACGTTGAA
  GAAAATGCTGTTGAACGTGGAGCAAATGGTTATAATTTTAACGAAAATGTACAAGCTTTT
  AAATTTGCCAAAGATGCAAAAGTTAACGACAAAATTAAAGTTATTGGGTACCCTTTACCA
  GCTCAAAATACATTCAAACAATTTGAATCAACTGGAACTGTAAAAAGTATTAAAGATAAT
  AATTTAAATTTTGATGCATATATCGAGCCTGGAAATTCAGGATCTCCAGTTTTAAATTCA
  AATAATGAAGTTGTAGGAGTTGTGTATGGAGGTATTGGAAAAATCGGATCAGAATACAAT
  GGTGCAGTTTATTTTACGCCACAAATCAAAGAATTTATTCAAAAGCACATTGAACAATAA
  60
  120
  180
  240
  300
  360
  420
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  540
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  720
  

⊟Protein

⊟General

• locus tag: JSNZ_001808
• symbol: SplC
• description: serine protease SplC
• length: 239
• theoretical pI: 9.04903
• theoretical MW: 26112.2
• GRAVY: -0.474895

⊟Function

• reaction:
  EC 3.4.21.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 16.4)
  Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 16.4)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 15.1)
  Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 15.1)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 86.1)
  and 6 more
  Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 60.3)
  Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 19.2)
  Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 19)
  Mycop_pep_DUF31; Mycoplasma peptidase (DUF31) (PF01732; HMM-score: 17.5)
  Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 14.8)
  Mtd (CL0737) K1-lyase_N; K1 capsule-specific polysaccharide lyase, N-terminal domain (PF24145; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.72
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.0001
  • Cell wall & surface Score: 0.0024
  • Extracellular Score: 0.9975
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 36 aa
  • SP(Sec/SPI): 0.759932
  • TAT(Tat/SPI): 0.004302
  • LIPO(Sec/SPII): 0.115285
  • Cleavage Site: CS pos: 36-37. AKA-EK. Pr: 0.4900
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MNKNVVIKSIAALTILTSITGIGDSVREEKQQIAKAEKNVTQVKDTNVSPYKGVVAFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKIKNISDYPDNEDISVMNVEENAVERGANGYNFNENVQAFKFAKDAKVNDKIKVIGYPLPAQNTFKQFESTGTVKSIKDNNLNFDAYIEPGNSGSPVLNSNNEVVGVVYGGIGKIGSEYNGAVYFTPQIKEFIQKHIEQ

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR (activation) regulon

  SaeR

  (TF)

  important in Virulence; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications