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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001808
  • pan locus tag?: SAUPAN004564000
  • symbol: splC
  • pan gene symbol?: splC
  • synonym:
  • product: serine protease SplC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001808
  • symbol: splC
  • product: serine protease SplC
  • replicon: chromosome
  • strand: -
  • coordinates: 1854999..1855718
  • length: 720
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAAAAACGTAGTTATTAAAAGTATTGCAGCTTTGACCATATTAACATCTATAACT
    GGCATAGGTGATTCAGTCCGAGAAGAAAAACAACAAATAGCGAAAGCTGAGAAAAATGTT
    ACACAAGTTAAAGATACCAATGTCTCTCCATATAAAGGTGTTGTGGCATTTAAAGATGCG
    ACTGGTTTTGTGATTGGTAAAAATACCATTATCACTAATAAACATGTATCGAAAGATTAC
    AAAGTGGGTGATCGAATTACCGCGCACCCAAATGGCGACAAAGGCAACGGTGGCATTTAT
    AAAATTAAAAATATTTCTGATTATCCAGATAATGAGGATATATCAGTAATGAACGTTGAA
    GAAAATGCTGTTGAACGTGGAGCAAATGGTTATAATTTTAACGAAAATGTACAAGCTTTT
    AAATTTGCCAAAGATGCAAAAGTTAACGACAAAATTAAAGTTATTGGGTACCCTTTACCA
    GCTCAAAATACATTCAAACAATTTGAATCAACTGGAACTGTAAAAAGTATTAAAGATAAT
    AATTTAAATTTTGATGCATATATCGAGCCTGGAAATTCAGGATCTCCAGTTTTAAATTCA
    AATAATGAAGTTGTAGGAGTTGTGTATGGAGGTATTGGAAAAATCGGATCAGAATACAAT
    GGTGCAGTTTATTTTACGCCACAAATCAAAGAATTTATTCAAAAGCACATTGAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001808
  • symbol: SplC
  • description: serine protease SplC
  • length: 239
  • theoretical pI: 9.04903
  • theoretical MW: 26112.2
  • GRAVY: -0.474895

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 16.4)
    Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 16.4)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 15.1)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 15.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 86.1)
    and 6 more
    Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 60.3)
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 19.2)
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 19)
    Mycop_pep_DUF31; Mycoplasma peptidase (DUF31) (PF01732; HMM-score: 17.5)
    Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 14.8)
    Mtd (CL0737) K1-lyase_N; K1 capsule-specific polysaccharide lyase, N-terminal domain (PF24145; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.0024
    • Extracellular Score: 0.9975
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 36 aa
    • SP(Sec/SPI): 0.759932
    • TAT(Tat/SPI): 0.004302
    • LIPO(Sec/SPII): 0.115285
    • Cleavage Site: CS pos: 36-37. AKA-EK. Pr: 0.4900
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNKNVVIKSIAALTILTSITGIGDSVREEKQQIAKAEKNVTQVKDTNVSPYKGVVAFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKIKNISDYPDNEDISVMNVEENAVERGANGYNFNENVQAFKFAKDAKVNDKIKVIGYPLPAQNTFKQFESTGTVKSIKDNNLNFDAYIEPGNSGSPVLNSNNEVVGVVYGGIGKIGSEYNGAVYFTPQIKEFIQKHIEQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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