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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001806
  • pan locus tag?: SAUPAN001332000
  • symbol: splE2
  • pan gene symbol?: splE2
  • synonym:
  • product: serine protease

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001806
  • symbol: splE2
  • product: serine protease
  • replicon: chromosome
  • strand: -
  • coordinates: 1853275..1853991
  • length: 717
  • essential: unknown

Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAAAAATATAATCATCAATAGTATTGCAGCATTGACGATTTTAACATCAGTGACT
    GGTGTCGGCACAACAATTATTGAAGGTATTCAACAAACGGCTAAAGCTGAACATAATGTG
    ACACAAGTACAAAACACAAGTATCTCACCTTATAAAGGTATTGTTAGAATTGGATCAGCT
    ACAGGATTTGTGGTAGGAAAAAACACAATTTTAACTAATAAGCATGTATTACCAGGAGTT
    CAAGTTGGTAGTACAATTATTGCTCATCCAAATGGCGAAAATGATACAGGTGGATTCTAT
    AAAGTTAAAAAAATCATTCCATACGCAGGAACAGAAGATTTAGCAATAGTTCAAGTTGAA
    GAAGATTCTGTTTATCCAAAAAATAAAAAATTCAGTGAAAATACCGAAGTCCTTAAATTT
    GCGAGTGGTGCTAAAGTAAATGATCGATTGTCAATCGTTGGTTATCCTAAACCTTACAAA
    AATAAGCATCATATGTTTGAATCAACTGGAAAAGTATTATCAATCGATGGTAATCAAATG
    ACTTCAGACGCATTTGCTGAAGGCGGTAATTCAGGATCACCAGTGTTTAATTCAAATCAT
    GAAGTAATTGCAATTGCATATGCTGTCGATGTCAAAAATGATGCAACAAAAAAATCATAT
    TTAGTTTATTTCACACCAGAAATCAAGAAATTCATCGCAGATAACACAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    717

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: JSNZ_001806
  • symbol: SplE2
  • description: serine protease
  • length: 238
  • theoretical pI: 9.35233
  • theoretical MW: 25680
  • GRAVY: -0.217647

Function[edit | edit source]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination sporulation peptidase YabG (TIGR02855; HMM-score: 18.6)
  • TheSEED:
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 67.4)
    Trypsin; Trypsin (PF00089; HMM-score: 65.5)
    and 4 more
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 18.5)
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 17.4)
    Mycop_pep_DUF31; Mycoplasma peptidase (DUF31) (PF01732; HMM-score: 16.3)
    no clan defined Peptidase_U57; YabG peptidase U57 (PF05582; HMM-score: 16)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helix: 1
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.0029
    • Extracellular Score: 0.997
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 36 aa
    • SP(Sec/SPI): 0.888966
    • TAT(Tat/SPI): 0.008542
    • LIPO(Sec/SPII): 0.026938
    • Cleavage Site: CS pos: 36-37. AKA-EH. Pr: 0.7556
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

Protein sequence[edit | edit source]

  • MNKNIIINSIAALTILTSVTGVGTTIIEGIQQTAKAEHNVTQVQNTSISPYKGIVRIGSATGFVVGKNTILTNKHVLPGVQVGSTIIAHPNGENDTGGFYKVKKIIPYAGTEDLAIVQVEEDSVYPKNKKFSENTEVLKFASGAKVNDRLSIVGYPKPYKNKHHMFESTGKVLSIDGNQMTSDAFAEGGNSGSPVFNSNHEVIAIAYAVDVKNDATKKSYLVYFTPEIKKFIADNTDK

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]