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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001807
  • pan locus tag?: SAUPAN004563000
  • symbol: splE3
  • pan gene symbol?: splE3
  • synonym:
  • product: serine protease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001807
  • symbol: splE3
  • product: serine protease
  • replicon: chromosome
  • strand: -
  • coordinates: 1854161..1854877
  • length: 717
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAAAAATATAATCATCAAAAGTATTGCGGCATTGACGATTTTAACATCAGTGACT
    GGTGTCGGCACAACAGTGGTTGAGGGTATTCAACAAACGGCTAAAGCTGAACATAACGTG
    ACGAAGATAACGAATACTAGTATTTCGCCATACAAAGGCATATTAAGAGTAGGTGCAGGT
    ACAGGTTTTGTAGTAGGTAAAAATACCATTTTAACCAATAGACATGTCGCGAAAGATGTT
    CAAGTAGGCAGTACGGTTCTTGCCCACCCGAATGGCGAAAATGATACTGGCGGATACTAT
    AAAGTCAAAAAAGTTATCCCATATGCAGGCTCTGCAGATTTAGCTATTGTTCAAGTTGAA
    GAAGATTCAGTTTATCCAAAAAATAAAAAGTTCGGTGAAAATACTGAAATCCTAACGCTG
    ACATCGGAAGTTAAAGCGAATGAAAGAATTGCTATTGTTGGATATCCAGCTCCATATAAA
    AATAAACATCATATGTATCAATCAACAGGAACAGTACTATCAATTAATGGAGATAAATTA
    GTATCAGATGCATTTGCTGAAGGTGGCAATTCAGGGTCACCGGTCTTTAACAATAAAAAT
    GAAGTTGTGGGCGTTTTATATAGTGGAGACCAAATTGGTCATTCTAAAAAAGAGTCATAC
    AGCGTATATTTAACGCCTGAAATTAAGAAATTCATTGCAGATAACATAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    717

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001807
  • symbol: SplE3
  • description: serine protease
  • length: 238
  • theoretical pI: 9.4709
  • theoretical MW: 25514.7
  • GRAVY: -0.234874

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 18.8)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 18.8)
  • TheSEED:
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 71.3)
    Trypsin; Trypsin (PF00089; HMM-score: 63.4)
    and 5 more
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 23.6)
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 20.4)
    Mycop_pep_DUF31; Mycoplasma peptidase (DUF31) (PF01732; HMM-score: 18.9)
    Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 14.9)
    Peptidase_S3; Alphavirus core protein (PF00944; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.0023
    • Extracellular Score: 0.9976
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 36 aa
    • SP(Sec/SPI): 0.926616
    • TAT(Tat/SPI): 0.006667
    • LIPO(Sec/SPII): 0.026952
    • Cleavage Site: CS pos: 36-37. AKA-EH. Pr: 0.6984
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVTKITNTSISPYKGILRVGAGTGFVVGKNTILTNRHVAKDVQVGSTVLAHPNGENDTGGYYKVKKVIPYAGSADLAIVQVEEDSVYPKNKKFGENTEILTLTSEVKANERIAIVGYPAPYKNKHHMYQSTGTVLSINGDKLVSDAFAEGGNSGSPVFNNKNEVVGVLYSGDQIGHSKKESYSVYLTPEIKKFIADNIDK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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