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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1631 [new locus tag: SA_RS09180 ]
  • pan locus tag?: SAUPAN004567000
  • symbol: splA
  • pan gene symbol?: splA
  • synonym:
  • product: serine protease SplA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1631 [new locus tag: SA_RS09180 ]
  • symbol: splA
  • product: serine protease SplA
  • replicon: chromosome
  • strand: -
  • coordinates: 1864410..1865117
  • length: 708
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAAAAATGTAATGGTTAAAGGTTTAACTGCTTTAACTATTTTAACATCTCTTGGT
    TTCGCTGAAAATATTTCTAATCAGCCTCATTCAATTGCCAAAGCAGAAAAGAATGTCAAA
    GAAATTACCGATGCAACTAAGGCACCATACAATTCAGTGGTAGCATTTGCGGGTGGTACT
    GGTGTAGTTGTTGGTAAAAATACAATCGTAACTAACAAACATATCGCTAAAAGTAATGAT
    ATTTTTAAAAATAGAGTAGCAGCACATTATTCGAGTAAAGGTAAAGGCGGAGGAAACTAC
    GACGTTAAAGACATTGTAGAATATCCTGGAAAAGAAGACCTTGCGATAGTTCATGTTCAT
    GAAACAAGTACAGAAGGTTTGAATTTTAATAAGAACGTTAGTTATACAAAATTTGCAGAA
    GGAGCAAAAGCGAAAGATAGAATTTCTGTTATTGGTTATCCAAAGGGTGCACAAACAAAA
    TATAAAATGTTTGAATCGACAGGAACGATTAACCATATCAGTGGAACGTTTATAGAATTT
    GATGCGTATGCACAACCAGGTAATTCAGGATCTCCTGTATTGAATTCTAAACATGAACTG
    ATTGGTATTTTATATGCAGGTAGTGGAAAAGATGAATCTGAAAAGAATTTCGGTGTTTAT
    TTCACACCACAATTAAAAGAATTTATTCAAAATAATATTGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    708

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1631 [new locus tag: SA_RS09180 ]
  • symbol: SplA
  • description: serine protease SplA
  • length: 235
  • theoretical pI: 9.61883
  • theoretical MW: 25440.5
  • GRAVY: -0.398723

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 28.4)
    Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 28.4)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 25.2)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 25.2)
  • TheSEED  :
    • serine protease
  • PFAM:
    Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 72.1)
    Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 59.2)
    and 4 more
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 26.6)
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 19.6)
    Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 18.6)
    Peptidase_S39; Peptidase S39 (PF02122; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NISNQPHS
  • SignalP: Signal peptide SP(Sec/SPI) length 35 aa
    • SP(Sec/SPI): 0.916215
    • TAT(Tat/SPI): 0.013109
    • LIPO(Sec/SPII): 0.019625
    • Cleavage Site: CS pos: 35-36. AKA-EK. Pr: 0.5721
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITDATKAPYNSVVAFAGGTGVVVGKNTIVTNKHIAKSNDIFKNRVAAHYSSKGKGGGNYDVKDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFAEGAKAKDRISVIGYPKGAQTKYKMFESTGTINHISGTFIEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFGVYFTPQLKEFIQNNIEK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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