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FunGene: 08-OCT-2024
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus JSNZ
- locus tag: JSNZ_000564
- pan locus tag?: SAUPAN002450000
- symbol: JSNZ_000564
- pan gene symbol?: —
- synonym:
- product: DUF443 family protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: JSNZ_000564
- symbol: JSNZ_000564
- product: DUF443 family protein
- replicon: chromosome
- strand: +
- coordinates: 602391..603032
- length: 642
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID:
- RefSeq:
- BioCyc:
- MicrobesOnline:
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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601TTGCTTTGTGAAACTGAAAATATTAATAAGAATCCCAAATATAGAATTATCAAATACAAA
GATGAATATTTGATGATTGATTTAGTAAGTACATGGTTAGCACTCTTTTTCCCAATGATT
AATTGGCTGATTCCAAAAAAGTACTTCAAAATCAGCGAAAAAGATTTTGAAACTTTAAAC
ATTGTGAAGACAACTAAAATCAATTCTTTTTGGCCAGTGGCAGGAAGTACGGTCTTATTT
GGTGTTATGTTAAGAAGGTATTCCCATTTATTTATCGTTAAATATGAATATAGTATAGTA
ATTTTAATTTGTTGCATCATAATACTAGGTATTTTTCTGTTTTTTTTATATTTAAATCAA
AAGTTAAAGTTACAAATCTATAATGAAAACAAAAATAAAAGCAATAAGATAATCATATTT
CCCACTTTAAAGAGTCTTTGTTTATCAATAGTTTTATATATTTATTTAGGTGGTGGTTCA
TTCTTTACTATTTATATGTTATTGACGATTGAAGTTCAAAATATAATATTATTTATAACT
TTGTTTGTAATATTTTTGTTTTTCTTATTTTTAAATATGTGCTCACTATATGACAATAAA
GTTCATGTATTATTTAAATCAAATGGAATTGAAAAGTTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: JSNZ_000564
- symbol: JSNZ_000564
- description: DUF443 family protein
- length: 213
- theoretical pI: 9.61605
- theoretical MW: 25291.4
- GRAVY: 0.638967
⊟Function[edit | edit source]
- TIGRFAM: tandem five-TM protein (TIGR01218; HMM-score: 166.4)and 1 moreCcmD family protein (TIGR04391; HMM-score: 14)
- TheSEED: data available for COL, NCTC8325, Newman, USA300_FPR3757
- PFAM: no clan defined DUF443; Protein of unknown function (DUF443) (PF04276; HMM-score: 190.7)and 1 moreDUF4519; Domain of unknown function (DUF4519) (PF15012; HMM-score: 12.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 4
- DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0.002
- Cytoplasmic Membrane Score: 0.9647
- Cell wall & surface Score: 0.0001
- Extracellular Score: 0.0333
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002273
- TAT(Tat/SPI): 0.000135
- LIPO(Sec/SPII): 0.000486
- predicted transmembrane helices (TMHMM): 5
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq:
- UniProt:
⊟Protein sequence[edit | edit source]
- MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLIPKKYFKISEKDFETLNIVKTTKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEYSIVILICCIIILGIFLFFLYLNQKLKLQIYNENKNKSNKIIIFPTLKSLCLSIVLYIYLGGGSFFTIYMLLTIEVQNIILFITLFVIFLFFLFLNMCSLYDNKVHVLFKSNGIEKF
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- Operon-mapper [1] : JSNZ_000554 > JSNZ_000555 > JSNZ_000556 > JSNZ_000557 > JSNZ_000558 > JSNZ_000559 > JSNZ_000560 > JSNZ_000561 > JSNZ_000562 > JSNZ_000563 > JSNZ_000564 > JSNZ_000565 > JSNZ_000566 > JSNZ_000567
⊟Regulation[edit | edit source]
- regulator: LacR* (repression) regulon
LacR* (TF) important in Lactose utilization; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p)