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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000565
  • pan locus tag?: SAUPAN002444000
  • symbol: JSNZ_000565
  • pan gene symbol?: —
  • synonym:
  • product: DUF443 domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000565
  • symbol: JSNZ_000565
  • product: DUF443 domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 603069..603704
  • length: 636
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    TTGCTATGTGAATCTAAAGTTATCAACAAAAATCCTAAGTATAGAGTTATTAAATATGGT
    GATGAATATTTAATGATTGATTTAGTAAGTACCTGGTTAACTTTATTTCTTCCTATGATT
    AATTGGTTAATTCCAAAAAAATATGTCAAAATCAGTAAAAAAGAATTTGACGATTTAAAC
    ATTGTCAAACCTGTTAAAAATAAAGCTTTTTGGCCAGTTGCAGGTAGTACTATTTTGTTC
    GGAGTTACGTTTAGAAAATATATTCCTTCACTTAATATTCAATTAGAGAAAAACATGGTG
    ATTGTAATATGTTGTGCAATATTTCTGGGTGTTTTAATACTTTTTTTATTTCTGAATCGT
    AAGCTAAGGTTGGAAATTTATAATAATAACTCTAGTAAAGGGAAAATAATTTTATTTCCT
    TCATTAAAAAACTTTTGTTTCACAATATTTTATTATTTTTTATTTGGCGGTCTTTCAATA
    ATGGCTCTAAGTATGTTATTAACTTTAAATCCTCAAAATATAATAGGCTTTATTGGTTGG
    TTGGTAATGACTGCAGGTTTCTTTCTGTTAAACATGTCATCGATTATTGACAAAAAAATT
    TATGTATTATCTAAAACTAACACGGTGGAAAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    636

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000565
  • symbol: JSNZ_000565
  • description: DUF443 domain-containing protein
  • length: 211
  • theoretical pI: 10.2473
  • theoretical MW: 24428.5
  • GRAVY: 0.535545

⊟Function[edit | edit source]

  • TIGRFAM:
    tandem five-TM protein (TIGR01218; HMM-score: 174.8)
  • TheSEED: data available for COL, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined DUF443; Protein of unknown function (DUF443) (PF04276; HMM-score: 231.4)
    and 4 more
    DUF5357; Family of unknown function (DUF5357) (PF17310; HMM-score: 15.8)
    Baculo_11_kDa; Baculovirus 11 kDa family (PF06143; HMM-score: 13)
    CcmD_alt; CcmD family protein (PF20077; HMM-score: 12.5)
    Blr_divisome; Divisome-associated membrane protein Blr family (PF24673; HMM-score: 10.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.001
    • Cytoplasmic Membrane Score: 0.9682
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0305
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003941
    • TAT(Tat/SPI): 0.00019
    • LIPO(Sec/SPII): 0.000681
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLIPKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIVICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSIMALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LacR* (repression) regulon
    LacR*(TF)important in Lactose utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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