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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000533
  • pan locus tag?: SAUPAN002353000
  • symbol: JSNZ_000533
  • pan gene symbol?: vraB
  • synonym:
  • product: thiolase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000533
  • symbol: JSNZ_000533
  • product: thiolase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 576725..577864
  • length: 1140
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAATCAAGCAGTCATAGTTGCAGCTAAACGAACTGCATTTGGGAAATATGGTGGCACT
    TTAAAACATTTAGAGCCAGAACAATTGCTTAAACCTTTATTCCAACATTTTAAAGAGAAG
    TATCCAGAGGTAATATCTAAAATAGATGATGTAGTTTTAGGTAATGTTGTTGGGAATGGT
    GGCAATATTGCAAGAAAAGCATTGCTTGAAGCGGGGCTTAAAGATTCAATACCTGGCGTC
    ACAATCGATCGGCAATGTGGGTCTGGACTTGAAAGTGTTCAATATGCATGTCGCATGATC
    CAAGCCGGAGCTGGCAAGGTATATATTGCAGGTGGTGTTGAAAGTACAAGTCGAGCACCT
    TGGAAAATCAAACGACCGCATTCTGTGTACGAAACAGCATTACCTGAGTTTTATGAGCGT
    GCATCATTTGCACCTGAAATGAGCGACCCATCAATGATTCAAGGTGCTGAAAATGTGGCC
    AAGATGTATGATGTTTCAAGAGAATTACAAGATGAATTTGCTTATCGAAGTCATCAATTG
    ACAGCGGAAAATGTAAAGAATGGAAATATTTCTCAGGAAATATTACCTATAACCGTTAAA
    GGAGAAATATTCAACACTGATGAAAGTCTAAAATCACATATTCCGAAAGATAACTTTGGC
    CGATTTAAGCCCGTGATCAAAGGTGGGACCGTTACCGCTGCGAATAGTTGTATGAAAAAT
    GATGGTGCAGTTTTATTGCTTATTATGGAAAAAGATATGGCATACGAATTAGGTTTCGAG
    CATGGTTTATTATTTAAAGATGGTGTTACGGTAGGTGTTGATTCTAATTTTCCTGGCATT
    GGTCCAGTACCAGCCATTTCCAACTTACTAAAAAGAAATCAATTAACGATAGAAAATATT
    GAAGTCATTGAAATTAACGAAGCGTTCAGTGCACAGGTAGTTGCCTGCCAACAAGCTTTA
    AATATTTCAAATACGCAATTAAATATATGGGGTGGTGCATTAGCATCAGGTCATCCATAC
    GGTGCAAGCGGTGCCCAATTAGTGACTCGATTATTTTATATGTTTGACAAAGAGACTATG
    ATTGCATCTATGGGGATAGGGGGAGGTCTAGGAAATGCAGCATTATTTACTCGATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000533
  • symbol: JSNZ_000533
  • description: thiolase family protein
  • length: 379
  • theoretical pI: 6.6333
  • theoretical MW: 41093.8
  • GRAVY: -0.0957784

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.-?  ExPASy
  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Other acetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 383.7)
    and 4 more
    3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 261.3)
    Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 211.7)
    Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 126.2)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis beta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 15.9)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 189.6)
    and 2 more
    Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 104.7)
    ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 20.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9646
    • Cytoplasmic Membrane Score: 0.0163
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0188
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004423
    • TAT(Tat/SPI): 0.00021
    • LIPO(Sec/SPII): 0.000526
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQAGAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVAKMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: BirA* (repression) regulon
    BirA*(TF)important in Biotin biosynthesis;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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