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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000532
  • pan locus tag?: SAUPAN002352000
  • symbol: JSNZ_000532
  • pan gene symbol?: vraA
  • synonym:
  • product: AMP-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000532
  • symbol: JSNZ_000532
  • product: AMP-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 575347..576723
  • length: 1377
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAACGTAATTTTAGAACAGTTGAAAACACATACTCAAAATAAACCTAATGACATAGCA
    TTACATATCGATGATGAAACAATTACATATAGTCAACTAAATGCCCGCATCACTAGCGCA
    GTTGAATCTTTGCAGAAATATTCACTTAACCCTGTCGTTGCTATTAATATGAAATCACCG
    GTGCAAAGTATTATTTGTTATTTAGCTTTGCATCGTTTACATAAAGTGCCTATGATGATG
    GAAGGTAAATGGCAAAGTACTATACATCGTCAATTGATTGAAAAATATGGTATTAAAGAT
    GTAATTGGAGATACAGGTCTCATGCAGAATATAGACTCACCGATGTTTATTGATTCAACG
    CAATTACAGCACTACCCCAATTTATTACATATTGGTTTTACTTCAGGGACAACTGGACTG
    CCAAAAGCATATTATCGTGATGAAGATTCATGGTTGGCTTCTTTTGAAGTTAATGAAATG
    TTGATGTTAAAAAATGAAAATGCAATAGCAGCCCCTGGACCACTATCGCACTCGTTAACA
    TTATATGCGTTATTGTTTGCTTTAAGTTCCGGTCGTACTTTTATAGGACAGACCACTTTT
    CATCCTGAAAAGTTACTTAATCAATGTCATAAAATATCATCATACAAAGTTGCTATGTTT
    CTTGTTCCAACGATGATTAAATCATTATTGTTAGTTTACAACAATGAACATACAATCCAA
    TCATTTTTTAGCAGTGGAGATAAGCTGCATTCTTCTATTTTTAAAAAGATAAAAAATCAA
    GCAAATGACATAAATTTGATTGAATTTTTTGGTACATCGGAAACCAGTTTTATCAGCTAT
    AACTTGAATCAGCAAGCACCAGTTGAATCAGTAGGTGTGCTATTTCCAAATGTGGAATTG
    AAAACAACGAATCACGATCACAATGGTATAGGAACTATTTGTATAAAAAGTAATATGATG
    TTTAGTGGCTATGTAAGTGAACAATGTATAAATAATGATGAATGGTTTGTTACTAATGAT
    AATGGCTATGTAAAAGAGCAGTATTTATATTTAACGGGACGTCAACAGGATATGTTAATT
    ATTGGTGGTCAAAATATATATCCAGCACATGTTGAACGCCTTTTAACGCAATCTTCGAGC
    ATTGATGAAGCAATTATCATCGGTATTCCAAATGAGCGTTTTGGTCAAATAGGCGTATTG
    CTTTATTCTGGTGATGTTACACTTACACATAAAAATGTAAAACAATTTTTAAAAAAGAAA
    GTGAAACGCTATGAAATTCCATCGATGATTCATCATGTAGAAAAGATGTATTACACTGCA
    AGTGGTAAAATTGCTAGAGAAAAAATGATGTCGATGTATTTGAGAGGTGAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1377

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000532
  • symbol: JSNZ_000532
  • description: AMP-binding protein
  • length: 458
  • theoretical pI: 7.53263
  • theoretical MW: 52023.6
  • GRAVY: -0.186245

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 171.1)
    and 13 more
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 85.6)
    amino acid adenylation domain (TIGR01733; HMM-score: 78.7)
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 75.7)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 69.1)
    Metabolism Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 52.2)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 51.8)
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 47.4)
    benzoate-CoA ligase family (TIGR02262; HMM-score: 46.8)
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 22.9)
    Metabolism Energy metabolism Other phenylacetate-CoA ligase (TIGR02155; EC 6.2.1.30; HMM-score: 19.5)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 17.3)
    L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 15.8)
    Metabolism Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 11.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 103.8)
    and 3 more
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 29.8)
    POZ (CL0033) BTB; BTB/POZ domain (PF00651; HMM-score: 15.7)
    Pec_lyase-like (CL0268) DUF4097; Toastrack DUF4097 (PF13349; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8317
    • Cytoplasmic Membrane Score: 0.1607
    • Cell wall & surface Score: 0.0006
    • Extracellular Score: 0.007
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005925
    • TAT(Tat/SPI): 0.000246
    • LIPO(Sec/SPII): 0.000672
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESLQKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVIGDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEMLMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVAMFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNERFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMMSMYLRGEL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: BirA* (repression) regulon
    BirA*(TF)important in Biotin biosynthesis;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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