PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA0534 [new locus tag: SA_RS03095 ]
pan locus tag^?: SAUPAN002353000
symbol: vraB
pan gene symbol^?: vraB
• synonym:
• product: acetyl-CoA c-acetyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0534 [new locus tag: SA_RS03095 ]
• symbol: vraB
• product: acetyl-CoA c-acetyltransferase
• replicon: chromosome
• strand: +
• coordinates: 628718..629857
• length: 1140
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123339 NCBI
• RefSeq: NP_373787 NCBI
• BioCyc: see SA_RS03095
• MicrobesOnline: 102813 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAATCAAGCAGTCATAGTTGCAGCTAAACGAACTGCATTTGGGAAATATGGTGGCACT
  TTAAAACATTTAGAGCCAGAACAATTGCTTAAACCTTTATTCCAACATTTTAAAGAGAAG
  TATCCAGAGGTAATATCTAAAATAGATGATGTAGTTTTAGGTAATGTTGTTGGGAATGGT
  GGCAATATTGCAAGAAAAGCATTGCTTGAAGCGGGGCTTAAAGATTCAATACCTGGCGTC
  ACAATCGATCGGCAATGTGGGTCTGGACTTGAAAGTGTTCAATATGCATGTCGCATGATC
  CAAGCCGGAGCTGGCAAGGTATATATTGCAGGTGGTGTTGAAAGTACAAGTCGAGCACCT
  TGGAAAATCAAACGACCGCATTCTGTGTACGAAACAGCATTACCTGAGTTTTATGAGCGT
  GCATCATTTGCACCTGAAATGAGCGACCCATCAATGATTCAAGGTGCTGAAAATGTGGCC
  AAGATGTATGATGTTTCAAGAGAATTACAAGATGAATTTGCTTATCGAAGTCATCAACTG
  ACAGCGGAAAATGTAAAGAATGGAAATATTTCTCAGGAAATATTACCTATAACCGTTAAA
  GGAGAAATATTCAATACTGATGAAAGTCTAAAATCACATATTCCGAAAGATAACTTTGGC
  CGATTTAAGCCCGTAATCAAAGGTGGGACCGTTACCGCTGCGAATAGTTGTATGAAAAAT
  GATGGTGCAGTTTTATTGCTTATTATGGAAAAAGATATGGCATACGAATTAGGTTTCGAG
  CATGGTTTATTATTTAAAGATGGTGTTACGGTAGGTGTTGATTCTAATTTTCCTGGCATT
  GGTCCAGTACCAGCCATTTCCAACTTACTAAAAAGAAATCAATTAACGATAGAAAATATT
  GAAGTCATTGAAATTAACGAAGCGTTCAGTGCACAGGTAGTTGCCTGCCAACAAGCTTTA
  AATATTTCAAATACGCAATTAAATATATGGGGTGGTGCATTAGCATCAGGTCATCCATAC
  GGTGCAAGCGGTGCCCAATTAGTGACTCGATTATTTTATATGTTTGACAAAGAGACTATG
  ATTGCATCTATGGGGATAGGGGGAGGTCTAGGAAATGCAGCATTATTTACTCGATTCTAA
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⊟Protein

⊟General

• locus tag: SA0534 [new locus tag: SA_RS03095 ]
• symbol: VraB
• description: acetyl-CoA c-acetyltransferase
• length: 379
• theoretical pI: 6.6333
• theoretical MW: 41093.8
• GRAVY: -0.0957784

⊟Function

• reaction:
  EC 2.3.1.-?  ExPASy
• ⊞TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Other acetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 383.7)
  and 4 more
  3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 261.3)
  Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 211.7)
  Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 126.2)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis beta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 15.9)
• ⊞TheSEED  :
  • 3-ketoacyl-CoA thiolase (EC 2.3.1.16)
  • Acetyl-CoA acetyltransferase (EC 2.3.1.9)
  Amino Acids and Derivatives Branched-chain amino acids Isoleucine degradation  3-ketoacyl-CoA thiolase (EC 2.3.1.16)
  and 9 more
  Carbohydrates Fermentation Acetyl-CoA fermentation to Butyrate  Acetyl-CoA acetyltransferase (EC 2.3.1.9)
  Carbohydrates Fermentation Butanol Biosynthesis  Acetyl-CoA acetyltransferase (EC 2.3.1.9)
  Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  3-ketoacyl-CoA thiolase (EC 2.3.1.16)
  Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Acetyl-CoA acetyltransferase (EC 2.3.1.9)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Biotin Biotin biosynthesis  3-ketoacyl-CoA thiolase (EC 2.3.1.16)
  Fatty Acids, Lipids, and Isoprenoids Fatty Acids, Lipids, and Isoprenoids - no subcategory Polyhydroxybutyrate metabolism  3-ketoacyl-CoA thiolase (EC 2.3.1.16)
  Fatty Acids, Lipids, and Isoprenoids Fatty Acids, Lipids, and Isoprenoids - no subcategory Polyhydroxybutyrate metabolism  Acetyl-CoA acetyltransferase (EC 2.3.1.9)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids  Acetyl-CoA acetyltransferase (EC 2.3.1.9)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis  Acetyl-CoA acetyltransferase (EC 2.3.1.9)
• ⊞PFAM:
  Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 189.6)
  and 2 more
  Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 104.7)
  ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 20.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9646
  • Cytoplasmic Membrane Score: 0.0163
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.0188
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004423
  • TAT(Tat/SPI): 0.00021
  • LIPO(Sec/SPII): 0.000526
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926254 NCBI
• RefSeq: NP_373787 NCBI
• UniProt: Q7A768 UniProt

⊟Protein sequence

• MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQAGAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVAKMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0532 > vraA > vraB > vraC > SA0536

⊟Regulation

• ⊞regulator: BirA* (repression) regulon

  BirA*

  (TF)

  important in Biotin biosynthesis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications