PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000212
• pan locus tag^?: SAUPAN001157000
• symbol: JSNZ_000212
• pan gene symbol^?: rbsR
• synonym:
• product: LacI family DNA-binding transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000212
• symbol: JSNZ_000212
• product: LacI family DNA-binding transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 252251..253249
• length: 999
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGAAAAAAGTGTCAATTAAAGATGTTGCTAGAGAAGCTGGTGTATCAGTTACAACTGTG
  TCACATATTTTAAATCATCATGATAGTCGTTTTTCCGCAACAACGATAAAAAACGTACAT
  GCTGTTTCAGAACGTTTAGGCTATGCCCCTAATAAACATGCAAAACAATTGCGCGGCAGT
  AAAATTCAAACTATTGGCGTCATTTTGCCTAGCTTAACAAATCCGTTTTTCTCAGCACTG
  ATGCAAAGTATTCATGACCATAAACCATCTGATGTTGATTTATGCTTTTTAACATCTACA
  GCAACTGATTTGTATGACAATATTAAACATTTAATTGATCGAGGTATTGACGGATTAATT
  ATCGCACAATACATTTCATCCCCGGACGCCCTAAATAACTATCTAAAGAAACATCATGTA
  CCTTATGTCGTACTGGATCAAAATGACCATCAAGGCTATACAGATTTTGTTCGGACAAAT
  GAATATCAAGGCGGACAACTTGCAGCACAACATTTAATAGAACTCGGTCACAACAATATG
  ATGATTGTTGCACCATATGACATGATGGCGAATATGTCGACTCGTGTCGCTGGATTTGTT
  GATACTTTGCGCGCGAATCAATTGCCAGAACCACAAATCGTCCATACTGAATTATCTAAG
  CGCGGTGGGCTAACCATTGTTGATGACATCATGGTTCAATCTGCCACTGCAATCTTCGCT
  ATTAACGATGAACTCGCTATTGGCATTTTACGAGGACTAATTGAACATGGCATCAGTATT
  CCGAAAGATATCTCATTAATAGGTTATGACGACATTGATTATGCAGCGTACGTCTCGCCA
  CCTTTAACTACTGTGGCACAACCTATAACTGATATTGGCAAAACATCTTTAACCTTATTA
  CTTCAACGATTACAGCACTTAGATAAATCCATTGATATGATTGAATTACCAACGACTTTA
  AAAATTCGTGCAACAACTGGCTATCATCTTTCAAACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  999
  

⊟Protein

⊟General

• locus tag: JSNZ_000212
• symbol: JSNZ_000212
• description: LacI family DNA-binding transcriptional regulator
• length: 332
• theoretical pI: 6.76486
• theoretical MW: 36675.7
• GRAVY: -0.0728916

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 169.1)
  and 4 more
  Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 119.5)
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 71.6)
  Signal transduction Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 14)
  Genetic information processing Protein synthesis tRNA aminoacylation aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (TIGR00132; EC 6.3.5.-; HMM-score: 13)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 116.2)
  and 17 more
  Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 84.2)
  Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 59.4)
  HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 55.3)
  HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 20.1)
  HTH_3; Helix-turn-helix (PF01381; HMM-score: 18.6)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 15.9)
  HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 15.1)
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 13.7)
  HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 13.6)
  TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 13.5)
  Rv2175c_wHTH; DNA-binding protein Rv2175c, wHTH domain (PF21531; HMM-score: 12.8)
  HTH_10; HTH DNA binding domain (PF04967; HMM-score: 12.7)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 12.7)
  no clan defined PucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 12.5)
  HTH (CL0123) MerR; MerR family regulatory protein (PF00376; HMM-score: 11.9)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 11.7)
  no clan defined Pox_A8; VITF-3 subunit protein (PF04745; HMM-score: 10.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effector: Ribose
• genes regulated by RbsR*, TF important in Ribose utilization

  repression

  rbsK

  rbsD

  rbsU*

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9929
  • Cytoplasmic Membrane Score: 0.0023
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0047
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.025021
  • TAT(Tat/SPI): 0.005008
  • LIPO(Sec/SPII): 0.001402
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MKKVSIKDVAREAGVSVTTVSHILNHHDSRFSATTIKNVHAVSERLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTATDLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTNEYQGGQLAAQHLIELGHNNMMIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTELSKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAAYVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SigB (activation) regulon

  SigB

  (sigma factor)

  controlling a large regulon involved in stress/starvation response and adaptation; transcription unit predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)   other strains

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications