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FunGene: 08-OCT-2024

Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000211
  • pan locus tag?: SAUPAN001156000
  • symbol: JSNZ_000211
  • pan gene symbol?: rbsU
  • synonym:
  • product: GRP family sugar transporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000211
  • symbol: JSNZ_000211
  • product: GRP family sugar transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 251138..252019
  • length: 882
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAGCATTGTTGCATTACTTATCGGGTTAGGCCCCTTAATTGGCTGGGGATTCTTCCCA
    ACAGTCGCTTCAAAGTTTGGTGGTAAACCTGTACATCAAATTATCGGTGCTACTGTAGGT
    ACGTTAATCTTCGCTATTATATTAGCCGTAGTCACATCAAGTGGCTTCCCTACTGGAACA
    AATTTACTATTCGCCTTATTATCAGGTGCAGGATGGGGATTCGGACAAATCATTACATTT
    AAAGCGTTCGAATTAGTCGGCTCATCTCGTGCCATGCCAGTCACAACAGCATTCCAATTA
    TTAGGCGCATCTTTATGGGGTGTCTTTGCATTAGGAAATTGGCCGGGCATTGGTCATAAA
    ATCATTGGCTTTACAGCTTTAGTCGTTATTTTAATTGGGGCGCGTATGACAGTTTGGAGT
    GAACGCAAAGAAGCAAGTAACGCCAAAAATTTACGTCGTGCAGTGGTACTTCTGTTAATT
    GGTGAATTTGGATACTGGTTATATTCAGCTGCACCGCAAGCAACTTCTATTGATGGCCTA
    ACTGCCTTTTTACCTCAAGCAATGGGTATGGTAATTGTTGCAGTCATTTATGGCTTTATG
    AATATGGAAGCAGAGAATCCATTCCGTAATAAAATTACGTGGTTACAAATTATTTCAGGT
    TTCTTCTTTGCATTTGGTGCCTTAACATATCTTATTTCAGCACAACCTAATATGAATGGT
    TTAGCAACTGGATTTATTCTTTCTCAAACATCCGTTGTGCTTGCTACATTAACTGGTATT
    TATTTCTTAAAACAACATAAAACGTCAAAAGAAATGGTTATTACAATCATCGGCTTAGTA
    CTCATTTTAGTAGCCGCTTCTGTTACAGTATTTATAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    882

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: JSNZ_000211
  • symbol: JSNZ_000211
  • description: GRP family sugar transporter
  • length: 293
  • theoretical pI: 10.5857
  • theoretical MW: 31244
  • GRAVY: 0.900341

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids RhaT L-rhamnose-proton symporter family protein (TIGR00776; HMM-score: 257.8)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    DMT (CL0184) Sugar_transport; Sugar transport protein (PF06800; HMM-score: 369.6)
    and 3 more
    EamA; EamA-like transporter family (PF00892; HMM-score: 22.2)
    TMEM144; Transmembrane family, TMEM144 of transporters (PF07857; HMM-score: 16.6)
    no clan defined DUF4181; Domain of unknown function (DUF4181) (PF13789; HMM-score: 8.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9998
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0002
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.047956
    • TAT(Tat/SPI): 0.003449
    • LIPO(Sec/SPII): 0.032106
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

Protein sequence[edit | edit source]

  • MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIFAIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLGASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLIGEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMEAENPFRNKITWLQIISGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITIIGLVLILVAASVTVFIK

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulators: RbsR* (repression) regulon, CcpA regulon
    RbsR*(TF)important in Ribose utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

Literature[edit | edit source]

References[edit | edit source]

  1. Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]