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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00241
  • pan locus tag?: SAUPAN001156000
  • symbol: SAOUHSC_00241
  • pan gene symbol?: rbsU
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00241
  • symbol: SAOUHSC_00241
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 261269..262150
  • length: 882
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAGCATTGTTGCATTACTTATCGGGTTAGGCCCCTTAATTGGCTGGGGCTTCTTCCCA
    ACAGTCGCTTCAAAGTTTGGTGGTAAACCTGTACATCAAATTATCGGTGCTACTGTAGGT
    ACGTTAATCTTCGCTATTTTATTAGCCGTAGTCACATCAAGTGGCTTCCCTACTGGAACC
    AATTTGCTATTCGCCTTATTATCAGGTGCAGGATGGGGATTCGGACAAATCATTACATTT
    AAAGCGTTCGAATTAGTCGGCTCATCTCGTGCCATGCCAGTCACAACAGCATTCCAATTA
    TTAGGCGCATCTTTATGGGGTGTCTTTGCATTAGGAAATTGGCCAGGCATTGGTCATAAA
    ATCATTGGATTTACAGCTTTAGTCGTTATTCTAATTGGAGCGCGTATGACAGTTTGGAGT
    GAACGCAAAGAAGCAAGTAACGCCAAAAATTTACGTCGTGCAGTGGTACTTCTGTTAATT
    GGTGAATTTGGATACTGGTTATATTCAGCTGCACCGCAAGCAACTTCTATTGATGGCCTA
    ACTGCCTTTTTACCTCAAGCAATGGGTATGGTAATTGTTGCAGTCATTTATGGCTTTATG
    AATATGAAAGCAGAGAATCCATTCCGTAATAAAATTACGTGGTTACAAATTATTTCAGGT
    TTCTTCTTTGCATTTGGTGCTTTAACATATCTTATTTCAGCACAACCTAATATGAATGGT
    TTAGCAACTGGATTTATTCTTTCTCAAACATCCGTTGTGCTTGCTACATTAACTGGTATT
    TATTTCTTAAAACAACATAAAACGTCAAAAGAAATGGTTATTACAATCATCGGCTTAGTA
    CTCATTTTAGTAGCCGCTTCTGTTACAGTATTTATAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    882

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00241
  • symbol: SAOUHSC_00241
  • description: hypothetical protein
  • length: 293
  • theoretical pI: 10.7683
  • theoretical MW: 31243.1
  • GRAVY: 0.896587

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids RhaT L-rhamnose-proton symporter family protein (TIGR00776; HMM-score: 257.4)
  • TheSEED  :
    • Putative ribose uptake protein RbsU, GRP transporter family (TC 2.A.7.5)
    Carbohydrates Monosaccharides D-ribose utilization  Putative ribose uptake protein RbsU, GRP transporter family (TC 2.A.7.5)
  • PFAM:
    DMT (CL0184) Sugar_transport; Sugar transport protein (PF06800; HMM-score: 363)
    and 3 more
    EamA; EamA-like transporter family (PF00892; HMM-score: 22.1)
    TMEM144; Transmembrane family, TMEM144 of transporters (PF07857; HMM-score: 16.3)
    no clan defined HIG_1_N; Hypoxia induced protein conserved region (PF04588; HMM-score: 9.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.044794
    • TAT(Tat/SPI): 0.004541
    • LIPO(Sec/SPII): 0.032193
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIFAILLAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLGASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLIGEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQIISGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITIIGLVLILVAASVTVFIK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: RbsR* (repression) regulon, CcpA* regulon
    RbsR*(TF)important in Ribose utilization; compare RegPrecise for N315  [1]
    CcpA*(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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