From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000209
  • pan locus tag?: SAUPAN001154000
  • symbol: rbsK
  • pan gene symbol?: rbsK
  • synonym:
  • product: ribokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000209
  • symbol: rbsK
  • product: ribokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 249777..250691
  • length: 915
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACCAACAAAGTTGTTATTTTAGGTTCAACGAATGTCGATCAATTTTTAACAGTTGAA
    AGATATGCACAACCAGGCGAAACATTACATGTTGAAGAAGCACAAAAAGCATTCGGCGGA
    GGTAAAGGTGCCAACCAGGCTATTGCCACTGCACGCATGCAAGCAGACACAACATTTATT
    ACTAAAATTGGCACTGATGGCGTTGCTGATTTCATCTTAGAAGATTTTAAAGCGGCTCAT
    ATTGATACATCATATATTATCAAAACAACTGAAGCAAAAACGGGCCAAGCCTTTATCACT
    GTGAATGCAGAAGGACAAAACACCATCTATGTTTATGGTGGTGCGAATATGACGATGACA
    CCTGAAGATGTTATTAACGCAAAAGACGCTATAATCAATGCAGACTTTGTCGTTGCACAA
    TTAGAAGTACCCATCCCGGCTATTATATCTGCATTTGAAATTGCCAAGGCACATGGTGTG
    ACGACAGTATTAAATCCTGCACCAGCGAAAGCATTACCTAATGAATTATTATCATTAATC
    GATATTATTGTGCCAAACGAAACAGAAGCCGAATTGTTATCTGGGATTAAAGTAACTAAT
    GAACAATCTATGAAAGACAATGCTAATTACTTTTTATCTTTAGGCATTAAGACTGTTTTG
    ATTACGCTAGGTAAGCAAGGTACATATTTTGCTACTAAAAATCAAAGCCAACACATCGAA
    GCTTATAAAGTAAATGCGATTGATACAACTGCTGCAGGCGACACATTTATTGGTGCATTT
    GTCAGTCGCTTAAACAAGTCGCAAGATAACTTAGCAGATGCTATTGATTTTGGTAATAAA
    GCGAGCTCACTCACTGTACAAAAACACGGCGCGCAAGCATCTATTCCTCTACTAGAAGAA
    GTAAATCAAGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    915

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000209
  • symbol: RbsK
  • description: ribokinase
  • length: 304
  • theoretical pI: 4.6011
  • theoretical MW: 32450.5
  • GRAVY: 0.0101974

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.15?  ExPASy
    Ribokinase ATP + D-ribose = ADP + D-ribose 5-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 382.2)
    and 7 more
    Metabolism Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 116.5)
    hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 93.7)
    1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 86.1)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 83.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 52.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 26.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 25)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 230.5)
    and 3 more
    Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 52)
    NADP_Rossmann (CL0063) Glyco_hydro_4; Family 4 glycosyl hydrolase (PF02056; HMM-score: 14)
    E-set (CL0159) Adeno_E3_CR1; Adenovirus E3 region protein CR1 (PF02440; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9975
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.002
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014499
    • TAT(Tat/SPI): 0.001126
    • LIPO(Sec/SPII): 0.001169
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGANQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTTEAKTGQAFITVNAEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGVTTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKTVLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAIDFGNKASSLTVQKHGAQASIPLLEEVNQV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: RbsR* (repression) regulon, CcpA regulon
    RbsR*(TF)important in Ribose utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_000209&oldid=105850"