PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0258 [new locus tag: SA_RS01520 ]
• pan locus tag^?: SAUPAN001154000
• symbol: rbsK
• pan gene symbol^?: rbsK
• synonym:
• product: ribokinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0258 [new locus tag: SA_RS01520 ]
• symbol: rbsK
• product: ribokinase
• replicon: chromosome
• strand: -
• coordinates: 312952..313866
• length: 915
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123037 NCBI
• RefSeq: NP_373504 NCBI
• BioCyc: see SA_RS01520
• MicrobesOnline: 102530 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGACCAACAAAGTTGTTATTTTAGGTTCAACGAATGTCGATCAATTTTTAACAGTTGAA
  AGATATGCACAACCAGGCGAAACATTACATGTTGAAGAAGCACAAAAAGCATTCGGCGGA
  GGTAAAGGTGCCAACCAGGCTATTGCCACTGCACGCATGCAAGCAGACACAACATTTATT
  ACTAAAATTGGCACTGATGGCGTTGCTGATTTCATCTTAGAAGATTTTAAAGCGGCTCAT
  ATTGATACATCATATATTATCAAAACAACTGAAGCAAAAACGGGCCAAGCCTTTATCACT
  GTGAATGCAGAAGGACAAAACACCATCTATGTTTATGGTGGTGCGAATATGACGATGACA
  CCTGAAGATGTTATTAACGCAAAAGACGCTATAATCAATGCAGACTTTGTCGTTGCACAA
  TTAGAAGTACCCATCCCGGCTATTATATCTGCATTTGAAATTGCCAAGGCACATGGTGTG
  ACGACAGTATTAAATCCTGCACCAGCGAAAGCATTACCTAATGAATTATTATCATTAATC
  GATATTATTGTGCCAAACGAAACAGAAGCCGAATTGTTATCTGGGATTAAAGTAACTAAT
  GAACAATCTATGAAAGACAATGCCAATTACTTTTTATCTTTAGGCATTAAGACTGTTTTG
  ATTACGCTAGGTAAGCAAGGTACATATTTTGCTACTAAAAATCAAAGCCAACACATCGAA
  GCTTATAAAGTAAATGCGATTGATACAACTGCTGCAGGCGACACATTTATTGGTGCATTT
  GTCAGTCGCTTAAACAAGTCGCAAGATAACTTAGCAGATGCTATTGATTTTGGTAATAAA
  GCGAGCTCACTCACTGTACAAAAACACGGCGCGCAAGCATCTATTCCTCTACTAGAAGAA
  GTAAATCAAGTTTAA
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⊟Protein

⊟General

• locus tag: SA0258 [new locus tag: SA_RS01520 ]
• symbol: RbsK
• description: ribokinase
• length: 304
• theoretical pI: 4.6011
• theoretical MW: 32450.5
• GRAVY: 0.0101974

⊟Function

• reaction:
  EC 2.7.1.15?  ExPASy
  Ribokinase ATP + D-ribose = ADP + D-ribose 5-phosphate
• TIGRFAM:
  Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 382.2)
  and 7 more
  Metabolism Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 116.5)
  hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 93.7)
  1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 86.1)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 83.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 52.2)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 26.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 25)
• TheSEED  :
  • Ribokinase (EC 2.7.1.15)
  Carbohydrates Monosaccharides D-ribose utilization  Ribokinase (EC 2.7.1.15)
  and 1 more
  Carbohydrates Monosaccharides Deoxyribose and Deoxynucleoside Catabolism  Ribokinase (EC 2.7.1.15)
• PFAM:
  Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 228.7)
  and 4 more
  Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 50.8)
  NADP_Rossmann (CL0063) Glyco_hydro_4; Family 4 glycosyl hydrolase (PF02056; HMM-score: 14.1)
  no clan defined DUF2341; Domain of unknown function (DUF2341) (PF10102; HMM-score: 12.9)
  Ig (CL0011) Adeno_E3_CR1; Adenovirus E3 region protein CR1 (PF02440; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.014499
  • TAT(Tat/SPI): 0.001126
  • LIPO(Sec/SPII): 0.001169
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15925971 NCBI
• RefSeq: NP_373504 NCBI
• UniProt: A0A0H3JNI8 UniProt

⊟Protein sequence

• MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGANQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTTEAKTGQAFITVNAEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGVTTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKTVLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAIDFGNKASSLTVQKHGAQASIPLLEEVNQV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: rbsK < rbsD < SA0260

⊟Regulation

• regulators: RbsR* (repression) regulon, CcpA regulon

  RbsR*	(TF)	important in Ribose utilization; RegPrecise
  CcpA	(TF)	important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications