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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000172
  • pan locus tag?: SAUPAN001096000
  • symbol: JSNZ_000172
  • pan gene symbol?: fadX
  • synonym:
  • product: acyl CoA:acetate/3-ketoacid CoA transferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000172
  • symbol: JSNZ_000172
  • product: acyl CoA:acetate/3-ketoacid CoA transferase
  • replicon: chromosome
  • strand: -
  • coordinates: 210521..212098
  • length: 1578
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGAGGTGACTTGCTTGAAACAAATCACATGGCACGACTTACAACATATCATTAAAGAT
    GGTGATGTGATTGGTTTACCAGCATTAGCTGTAGCCAACTTACCCGCCGAAGTTCTACGT
    GCTGTGTTAGCGCAACATGACACATATCATACGCCCAATGATTTAACGTTTATATTAGCG
    AATGATATCCATAGTTTAGGTGCCGCACCGGATTTAGATGATTTTATAGAACGTCGCATG
    ATTAAACGTGTCATTATGAGCATTTTAACGGCTTCTTCCAAAACGGCACAAGCAATGAAA
    AATAATGACATTGAAGCTTATTTTTTACCACAAGGTATCATTGCAACTCATTATCGTCAG
    AGTAATCAATTATTACCTGGAGTTATTACTAAAATAGGATTAAACACAGCTGTTGATCCT
    AGATACGGTGGCGGTAAAGTAAATACACGAACAACTGATGATTTAGTTTCATTAGTAACC
    ATCAACGATGAAACATACTTACATTACACATTCCCTAGCGTTGATGTGGCACTACTGAGA
    GGAACATACGCAGATCAACAAGGTAACATTTATTTAACTCAAGAAGCGTACTTGAGCGAG
    TGTTATCATGTCGCATTAAACACGAAAGCCAATCATGGGAAAGTTATTGTACAAGTTAAA
    GCTTTAGTTGACGACTATCAACTAAAACCGAATGAAGTTGTTATCCCAGGAAATCTTGTC
    GATTATGTATACGTCACAGAAGATGAAAAGAATCACCGCCAAGTAATTCAGAGTCATTAT
    TTACCAGCCTTGTCTGGAGAAGAACGAATTGATGGAATACCTGAACCCGCATTACCTTTT
    AATAGTCGCAAATTGATTCTCCGACGTGCTGCTCAGTTTTTAACTTATGGCGATACAATT
    AGCATCGGTTATGGAATCAATAATGAACTCTCTAATTTATTGCATGAAGAATGTGTTGAA
    CATGATGTGCAACCGATTCTAGATGTTGGCATTTTCGGTGGATTCGTTGGGAGTCGTGAA
    CATTTTGGTATGAATTACAATGCAGATGTGCGCATGCCTCATGATCGAGCATGGGATTTT
    ATTTATAACAATGGTGTATCAGTTGCCTATCTTAGCTTTGCTGAGGTTGATCAATACGGC
    AATGTCAACGTGTCTTACTTCAATGACCGACTAAATGGATGTGGCGGCTTTATAGACATT
    ACGCAATCTGTAAATAAAATTATCTTTTCAGGTACTTTTGTAGCTGGCAGTCATGTCTCA
    TGCCATAATCAACGATTAAACATTGAAACTGAAGGACAAAACCAGAAATTTGTATCAGAT
    GTGAGCCATATCGACTTTAATGCACAATATTCACAATCACTCGAGCAAGAAGTCTATTTT
    GTTACTGATCGTGCAGTATTCGAACTCACCAATCAAGGCTTGAAACTAATTGAAATTGCA
    CCAGGTCTTGATTTGCATAAAGATATTTTGAATCAAATGGCTTTTAAACCAATTATTGCT
    GATCATTTAAAATTAATTGATACCAGCATTTACAAAGAAAAATGGGGACAACTTAAACAA
    TCAATTCATAAAGTATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1578

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000172
  • symbol: JSNZ_000172
  • description: acyl CoA:acetate/3-ketoacid CoA transferase
  • length: 525
  • theoretical pI: 5.49984
  • theoretical MW: 58892.2
  • GRAVY: -0.168571

⊟Function[edit | edit source]

  • TIGRFAM:
    3-oxoacid CoA-transferase, A subunit (TIGR02429; EC 2.8.3.-; HMM-score: 38.3)
    and 3 more
    3-oxoacid CoA-transferase, B subunit (TIGR02428; EC 2.8.3.-; HMM-score: 26.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds malonate decarboxylase, alpha subunit (TIGR01110; HMM-score: 24)
    Metabolism Energy metabolism Other malonate decarboxylase, alpha subunit (TIGR01110; HMM-score: 24)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) CoA_trans; Coenzyme A transferase (PF01144; HMM-score: 58.2)
    and 3 more
    Mal_decarbox_Al; Malonate decarboxylase, alpha subunit, transporter (PF16957; HMM-score: 21.7)
    AcetylCoA_hydro; Acetyl-CoA hydrolase/transferase N-terminal domain (PF02550; HMM-score: 17.1)
    no clan defined Curlin_rpt; Curlin associated repeat (PF07012; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.964
    • Cytoplasmic Membrane Score: 0.0191
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0166
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013776
    • TAT(Tat/SPI): 0.002257
    • LIPO(Sec/SPII): 0.000615
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLAQHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNNDIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVTINDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNTKANHGKVIVQVKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEPALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGGFVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLNGCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQYSQSLEQEVYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDTSIYKEKWGQLKQSIHKV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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