From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0229 [new locus tag: SAUSA300_RS01215 ]
  • pan locus tag?: SAUPAN001096000
  • symbol: SAUSA300_0229
  • pan gene symbol?: fadX
  • synonym:
  • product: putative acyl-CoA transferase FadX

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0229 [new locus tag: SAUSA300_RS01215 ]
  • symbol: SAUSA300_0229
  • product: putative acyl-CoA transferase FadX
  • replicon: chromosome
  • strand: -
  • coordinates: 275350..276927
  • length: 1578
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGAGGTGACTTGCTTGAAACAAATCACATGGCACGACTTACAACATATCATTAAAGAT
    GGTGATGTGATTGGTTTACCAGCATTAGCTGTAGCCAACTTACCCGCCGAAGTTCTACGT
    GCTGTGTTAGCGCAACATGACACATATCATACGCCCAATGATTTAACGTTTATATTAGCG
    AATGATATTCATAGTTTAGGTGCCGCACCGGATTTAGATGATTTTATAGAACGTCGCATG
    ATTAAACGTGTCATTATGAGTATTTTAACGGCTTCTTCCAAAACGGCACAAGCAATGAAA
    AATAATGACATTGAAGCTTATTTTTTACCACAAGGTATCATTGCAACTCATTATCGTCAG
    AGTAATCAATTATTACCTGGAGTTATTACTAAAATCGGATTAAACACAGCTGTTGATCCT
    AGATACGGTGGCGGTAAAGTAAATACACGAACAACGGATGATTTAGTTTCATTAGTAACC
    ATCAACGATGAAACATACTTACATTACACATTCCCTAGCGTTGATGTGGCACTACTGAGA
    GGAACATACGCAGATCAACAAGGTAACATTTATTTAACTCAAGAAGCGTACTTGAGCGAG
    TGTTATCATGTCGCATTAAACGCGAAAGCCAATCATGGGAAAGTTATTGTACAAGTTAAA
    GCTTTAGTTGACGACTATCAACTAAAACCGAATGAAGTTGTTATCCCAGGAAATCTTGTC
    GATTATGTATACGTCACAGAAGATGAAAAGAATCACCGCCAAGTAATTCAAAGTCATTAT
    TTACCAGCCTTGTCTGGAGAAGAACGAATTGATGGAATACCTGAACCCGCATTACCTTTT
    AATAGTCGCAAATTGATTCTCCGACGTGCTGCTCAGTTTTTAACTTATGGCGATACAATT
    AGCATCGGTTATGGCATCAATAATGAACTCTCTAATTTATTGCATGAAGAATGTGTTGAA
    CATGATGTGCAACCGATTTTAGATGTTGGCATTTTCGGTGGATTCGTTGGGAGTCGTGAA
    CATTTTGGTATGAATTACAATGCAGATGTGCGCATGCCTCATGATCGAGCATGGGATTTT
    ATTTATAACAATGGTGTATCAGTTGCCTATCTTAGCTTTGCTGAGGTTGATCAATACGGC
    AATGTCAACGTGTCTTACTTCAATGACCGACTAAATGGATGTGGTGGCTTTATAGACATT
    ACGCAATCTGTAAATAAAATTATCTTTTCAGGTACTTTTGTAGCTGGCAGTCATGTCTCA
    TGCCATAATCAACGATTAAACATTGAAACTGAAGGACAAAACCAGAAATTTGTATCAGAT
    GTGAGCCATATCGACTTTAATGCACAATATTCACAATCACTCGAGCAAGAAATCTATTTT
    GTTACTGATCGTGCAGTATTCGAACTCACCAATCAAGGCTTGAAACTAATTGAAATTGCA
    CCAGGTCTTGATTTGCATAAAGATATTTTGAATCAAATGGCTTTTAAACCAATTATTGCT
    GATCATTTAAAATTAATTGATACCAGCATTTACAAAGAAAAATGGGGACAACTTAAACAA
    TCAATTCATAAAGTATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1578

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0229 [new locus tag: SAUSA300_RS01215 ]
  • symbol: SAUSA300_0229
  • description: putative acyl-CoA transferase FadX
  • length: 525
  • theoretical pI: 5.49984
  • theoretical MW: 58876.2
  • GRAVY: -0.163238

⊟Function[edit | edit source]

  • TIGRFAM:
    3-oxoacid CoA-transferase, A subunit (TIGR02429; EC 2.8.3.-; HMM-score: 39.3)
    and 3 more
    3-oxoacid CoA-transferase, B subunit (TIGR02428; EC 2.8.3.-; HMM-score: 26.5)
    Metabolism Transport and binding proteins Cations and iron carrying compounds malonate decarboxylase, alpha subunit (TIGR01110; HMM-score: 23.7)
    Metabolism Energy metabolism Other malonate decarboxylase, alpha subunit (TIGR01110; HMM-score: 23.7)
  • TheSEED  :
    • FIG01108629: hypothetical protein
  • PFAM:
    ISOCOT_Fold (CL0246) CoA_trans; Coenzyme A transferase (PF01144; HMM-score: 71.4)
    and 3 more
    Mal_decarbox_Al; Malonate decarboxylase, alpha subunit, transporter (PF16957; HMM-score: 22.5)
    AcetylCoA_hydro; Acetyl-CoA hydrolase/transferase N-terminal domain (PF02550; HMM-score: 17.7)
    no clan defined Curlin_rpt; Curlin associated repeat (PF07012; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013776
    • TAT(Tat/SPI): 0.002257
    • LIPO(Sec/SPII): 0.000615
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLAQHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNNDIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVTINDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQVKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEPALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGGFVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLNGCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQYSQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDTSIYKEKWGQLKQSIHKV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_0229&oldid=98777"