From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000171
  • pan locus tag?: SAUPAN001095000
  • symbol: JSNZ_000171
  • pan gene symbol?: fadE
  • synonym:
  • product: class I adenylate-forming enzyme family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000171
  • symbol: JSNZ_000171
  • product: class I adenylate-forming enzyme family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 208990..210495
  • length: 1506
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAATTTCGATTGGATAAAAACGCGTTCAGACTTCGATGATGACAAGCCTGCCGTTATT
    GATCACGCAAAACAAACATCTTGGACATACCAACAACTCAATGCACGTGCTGATAATATG
    GCACATTATTTAACATCTCAAGGTGTTAAAAAAGGCGATGTTATCGGTATTTTTGCGCCA
    AATGATATTGCAATATTAGATTTATTATTTGCTTGTTTTAAAACAGGTGCGGTTTTTTTA
    CCATTGAATTGGCGGCTTAACCCAAAAGAAATTGCAGCCATTGTGGAAGATGCACAATTA
    AAACTGCTCTTCTATGCTGAAAAACATTTAAGTTCACTCACCGATATTGACCAAAACTTA
    TTGCATATGGATATTGATGTAGCGCAATATGATGAAATCGTAAATCCAGATTATCACCAA
    CCTTTTCAAGCAACACCTGTTGAACCACAAGATCTTGCAGCATTAATTTATACAAGTGGT
    ACAACCGGATCACCTAAAGGTGTGATGTTCTCATATGAATCATTTGTTCATAACGGTGCA
    AACTTAGAGCTGACGTATAAGTTCAATTCAAACTATATTACGATTGTATCAACACCAATG
    TTCCATGTTTTAGGGTTTAACGATACTGTATTACCAGTATTAATGTCAGGTGGCACACTT
    ATTCTTCAACGTTACTTTAACGGCGAAGAATTGAATGACATGATTGCACAATATCACCCT
    ACATTTATTATCATGATTCCGACAATGTATTATAGTACGCTACGTGCTAGTAATTTTAAT
    CCTGAAAATTTTAAAGCTATGGATTATATCATCCAAGGTGGTTCACAACCATTACCAAGT
    ATCCAAGCAGCTTTTAAACAATATGGCATAAACATTATTAACGGCTATGGTTTAACTGAA
    GCACCTCTTGTACTTGTTAATACACCGGAAAATTCAAAACGTAAGCCAATGAGTATTGGT
    AAAGCAGTCATGTTCGTTGATGCACGTATCCTTGATGATAACGGTGAGGAAGTACCTACT
    GGTGAGATTGGCGAACTTGCAATTAAGGCTAAAAATGTCACGCCAGGATATTGGAATAAA
    CCAGCAGAGACTGCCAAAGCATTTCATGGTCGTTATTTATTAACTGGTGACTTAGCGAAG
    ATGGACGACGATGGCGATATATTTATTATTGACCGCAAAAAAGAATTAATCATAACTGGT
    GGCGAAAATGTCTTACCATCTGAAGTCGAAAATGCTTTAGCTGAGCATCCACTAGTAGAC
    CGGTGTGTGGTCGTTGGCTATGATCATCCAAAATATGGTGAATCAATTGCTGCAGCCATT
    ATACTTCGCGAAGATGAACCTCATTACGCTGAAATTTTAGATCAACATATGCGAAGTCGT
    TTAGCAGGTTATAAAGTCCCAAGAATGTATGTACCAGTGACACATATGCCGTTAAACAGT
    ACGCAGAAACCAGATAAACTTGCGATTCGACAAATGATGAATGACAAAGTCTCACAAACA
    CTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1506

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000171
  • symbol: JSNZ_000171
  • description: class I adenylate-forming enzyme family protein
  • length: 501
  • theoretical pI: 5.01311
  • theoretical MW: 56178.8
  • GRAVY: -0.197605

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 270.9)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 249.6)
    amino acid adenylation domain (TIGR01733; HMM-score: 227.5)
    and 11 more
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 193.6)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 180.9)
    benzoate-CoA ligase family (TIGR02262; HMM-score: 166)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 153)
    Metabolism Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 143.6)
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 123.1)
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 69.4)
    L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 53.2)
    Metabolism Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 34.7)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 27.9)
    Metabolism Energy metabolism Other phenylacetate-CoA ligase (TIGR02155; EC 6.2.1.30; HMM-score: 11.5)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 274.2)
    and 3 more
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 52.9)
    AMP-binding_C_3; AMP-binding enzyme C-terminal domain (PF23562; HMM-score: 21.2)
    no clan defined UDI; Uracil-DNA glycosylase inhibitor (PF18880; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9814
    • Cytoplasmic Membrane Score: 0.0155
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0031
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009649
    • TAT(Tat/SPI): 0.00044
    • LIPO(Sec/SPII): 0.001227
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYLTSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKLLFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSGTTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGGTLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFKAMDYIIQGGSQPLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDNGEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIDRKKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYAEILDQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_000171&oldid=105812"